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Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum

Successfully developed in 1976, the continuous in vitro culture of Plasmodium falciparum has many applications in the field of malaria research. It has become an important experimental model that directly uses a human pathogen responsible for a high prevalence of morbidity and mortality in many part...

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Autor principal: Basco, Leonardo K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384318/
https://www.ncbi.nlm.nih.gov/pubmed/37513747
http://dx.doi.org/10.3390/pathogens12070900
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author Basco, Leonardo K.
author_facet Basco, Leonardo K.
author_sort Basco, Leonardo K.
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description Successfully developed in 1976, the continuous in vitro culture of Plasmodium falciparum has many applications in the field of malaria research. It has become an important experimental model that directly uses a human pathogen responsible for a high prevalence of morbidity and mortality in many parts of the world and is a major source of biological material for immunological, biochemical, molecular, and pharmacological studies. Until present, the basic techniques described by Trager and Jensen and Haynes et al. remain unchanged in many malaria research laboratories. Nonetheless, different factors, including culture media, buffers, serum substitutes and supplements, sources of erythrocytes, and conditions of incubation (especially oxygen concentration), have been modified by different investigators to adapt the original technique in their laboratories or enhance the in vitro growth of the parasites. The possible effects and benefits of these modifications for the continuous cultivation of asexual intraerythrocytic stages of P. falciparum, as well as future challenges in developing a serum-free cultivation system and axenic cultures, are discussed.
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spelling pubmed-103843182023-07-30 Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum Basco, Leonardo K. Pathogens Review Successfully developed in 1976, the continuous in vitro culture of Plasmodium falciparum has many applications in the field of malaria research. It has become an important experimental model that directly uses a human pathogen responsible for a high prevalence of morbidity and mortality in many parts of the world and is a major source of biological material for immunological, biochemical, molecular, and pharmacological studies. Until present, the basic techniques described by Trager and Jensen and Haynes et al. remain unchanged in many malaria research laboratories. Nonetheless, different factors, including culture media, buffers, serum substitutes and supplements, sources of erythrocytes, and conditions of incubation (especially oxygen concentration), have been modified by different investigators to adapt the original technique in their laboratories or enhance the in vitro growth of the parasites. The possible effects and benefits of these modifications for the continuous cultivation of asexual intraerythrocytic stages of P. falciparum, as well as future challenges in developing a serum-free cultivation system and axenic cultures, are discussed. MDPI 2023-07-01 /pmc/articles/PMC10384318/ /pubmed/37513747 http://dx.doi.org/10.3390/pathogens12070900 Text en © 2023 by the author. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Basco, Leonardo K.
Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum
title Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum
title_full Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum
title_fullStr Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum
title_full_unstemmed Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum
title_short Cultivation of Asexual Intraerythrocytic Stages of Plasmodium falciparum
title_sort cultivation of asexual intraerythrocytic stages of plasmodium falciparum
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384318/
https://www.ncbi.nlm.nih.gov/pubmed/37513747
http://dx.doi.org/10.3390/pathogens12070900
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