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Comparison of Xpert MTB/RIF Ultra Results of Stool and Sputum in Children with Presumptive Tuberculosis in Southern Ethiopia
The introduction of stool as a readily obtainable sample and the recently developed simple one-step (SOS) stool processing method on Xpert MTB/RIF Ultra (Xpert Ultra) offer an opportunity for TB diagnosis in children. We conducted this study in secondary health facilities in Ethiopia, which are the...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384425/ https://www.ncbi.nlm.nih.gov/pubmed/37505646 http://dx.doi.org/10.3390/tropicalmed8070350 |
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author | Babo, Yohannes Seremolo, Bihil Bogale, Mamush Bedru, Ahmed Wabe, Yasin Churako, Haba Bilat, Alemu Degaga, Tamiru de Haas, Petra Tiemersma, Edine Jerene, Degu |
author_facet | Babo, Yohannes Seremolo, Bihil Bogale, Mamush Bedru, Ahmed Wabe, Yasin Churako, Haba Bilat, Alemu Degaga, Tamiru de Haas, Petra Tiemersma, Edine Jerene, Degu |
author_sort | Babo, Yohannes |
collection | PubMed |
description | The introduction of stool as a readily obtainable sample and the recently developed simple one-step (SOS) stool processing method on Xpert MTB/RIF Ultra (Xpert Ultra) offer an opportunity for TB diagnosis in children. We conducted this study in secondary health facilities in Ethiopia, which are the first-level referral facilities for childhood TB diagnosis and treatment, with the aim to determine if stool-based TB diagnosis can be performed with a reasonable level of concordance with sputum tests using Xpert MTB/RIF Ultra. Eligible children 0–14 years old with presumptive pulmonary TB were asked to provide stools in addition to routinely requested sputum samples. We determined the level of agreement between the stool and sputum test results. Of the 373 children included in the study, 61% were <5 years of age and 56% were male. Thirty-six children (9.7%) were diagnosed with TB, and all started treatment. The rate of concordance between stool and sputum was high, with a kappa value of 0.83 (p < 0.001). There were more Xpert Ultra positive results on stool (n = 27 (7.2%)) than on sputum/NGA (n = 23 (6.2%)). Laboratories in secondary hospitals can perform stool-based TB diagnosis in children, with high concordance between stool and sputum test results reaffirming the applicability of the SOS stool method. |
format | Online Article Text |
id | pubmed-10384425 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103844252023-07-30 Comparison of Xpert MTB/RIF Ultra Results of Stool and Sputum in Children with Presumptive Tuberculosis in Southern Ethiopia Babo, Yohannes Seremolo, Bihil Bogale, Mamush Bedru, Ahmed Wabe, Yasin Churako, Haba Bilat, Alemu Degaga, Tamiru de Haas, Petra Tiemersma, Edine Jerene, Degu Trop Med Infect Dis Article The introduction of stool as a readily obtainable sample and the recently developed simple one-step (SOS) stool processing method on Xpert MTB/RIF Ultra (Xpert Ultra) offer an opportunity for TB diagnosis in children. We conducted this study in secondary health facilities in Ethiopia, which are the first-level referral facilities for childhood TB diagnosis and treatment, with the aim to determine if stool-based TB diagnosis can be performed with a reasonable level of concordance with sputum tests using Xpert MTB/RIF Ultra. Eligible children 0–14 years old with presumptive pulmonary TB were asked to provide stools in addition to routinely requested sputum samples. We determined the level of agreement between the stool and sputum test results. Of the 373 children included in the study, 61% were <5 years of age and 56% were male. Thirty-six children (9.7%) were diagnosed with TB, and all started treatment. The rate of concordance between stool and sputum was high, with a kappa value of 0.83 (p < 0.001). There were more Xpert Ultra positive results on stool (n = 27 (7.2%)) than on sputum/NGA (n = 23 (6.2%)). Laboratories in secondary hospitals can perform stool-based TB diagnosis in children, with high concordance between stool and sputum test results reaffirming the applicability of the SOS stool method. MDPI 2023-06-30 /pmc/articles/PMC10384425/ /pubmed/37505646 http://dx.doi.org/10.3390/tropicalmed8070350 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Babo, Yohannes Seremolo, Bihil Bogale, Mamush Bedru, Ahmed Wabe, Yasin Churako, Haba Bilat, Alemu Degaga, Tamiru de Haas, Petra Tiemersma, Edine Jerene, Degu Comparison of Xpert MTB/RIF Ultra Results of Stool and Sputum in Children with Presumptive Tuberculosis in Southern Ethiopia |
title | Comparison of Xpert MTB/RIF Ultra Results of Stool and Sputum in Children with Presumptive Tuberculosis in Southern Ethiopia |
title_full | Comparison of Xpert MTB/RIF Ultra Results of Stool and Sputum in Children with Presumptive Tuberculosis in Southern Ethiopia |
title_fullStr | Comparison of Xpert MTB/RIF Ultra Results of Stool and Sputum in Children with Presumptive Tuberculosis in Southern Ethiopia |
title_full_unstemmed | Comparison of Xpert MTB/RIF Ultra Results of Stool and Sputum in Children with Presumptive Tuberculosis in Southern Ethiopia |
title_short | Comparison of Xpert MTB/RIF Ultra Results of Stool and Sputum in Children with Presumptive Tuberculosis in Southern Ethiopia |
title_sort | comparison of xpert mtb/rif ultra results of stool and sputum in children with presumptive tuberculosis in southern ethiopia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384425/ https://www.ncbi.nlm.nih.gov/pubmed/37505646 http://dx.doi.org/10.3390/tropicalmed8070350 |
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