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Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties

We report the ability of the crude biosurfactant (BS B3-15), produced by the marine, thermotolerant Bacillus licheniformis B3-15, to hinder the adhesion and biofilm formation of Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 to polystyrene and human cells. First, we attempted...

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Autores principales: Zammuto, Vincenzo, Rizzo, Maria Giovanna, De Pasquale, Claudia, Ferlazzo, Guido, Caccamo, Maria Teresa, Magazù, Salvatore, Guglielmino, Salvatore Pietro Paolo, Gugliandolo, Concetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384595/
https://www.ncbi.nlm.nih.gov/pubmed/37513014
http://dx.doi.org/10.3390/microorganisms11071842
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author Zammuto, Vincenzo
Rizzo, Maria Giovanna
De Pasquale, Claudia
Ferlazzo, Guido
Caccamo, Maria Teresa
Magazù, Salvatore
Guglielmino, Salvatore Pietro Paolo
Gugliandolo, Concetta
author_facet Zammuto, Vincenzo
Rizzo, Maria Giovanna
De Pasquale, Claudia
Ferlazzo, Guido
Caccamo, Maria Teresa
Magazù, Salvatore
Guglielmino, Salvatore Pietro Paolo
Gugliandolo, Concetta
author_sort Zammuto, Vincenzo
collection PubMed
description We report the ability of the crude biosurfactant (BS B3-15), produced by the marine, thermotolerant Bacillus licheniformis B3-15, to hinder the adhesion and biofilm formation of Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 to polystyrene and human cells. First, we attempted to increase the BS yield, optimizing the culture conditions, and evaluated the surface-active properties of cell-free supernatants. Under phosphate deprivation (0.06 mM) and 5% saccharose, the yield of BS (1.5 g/L) increased by 37%, which could be explained by the earlier (12 h) increase in lchAA expression compared to the non-optimized condition (48 h). Without exerting any anti-bacterial activity, BS (300 µg/mL) prevented the adhesion of P. aeruginosa and S. aureus to polystyrene (47% and 36%, respectively) and disrupted the preformed biofilms, being more efficient against S. aureus (47%) than P. aeruginosa (26%). When added to human cells, the BS reduced the adhesion of P. aeruginosa and S. aureus (10× and 100,000× CFU/mL, respectively) without altering the epithelial cells’ viability. As it is not cytotoxic, BS B3-15 could be useful to prevent or remove bacterial biofilms in several medical and non-medical applications.
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spelling pubmed-103845952023-07-30 Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties Zammuto, Vincenzo Rizzo, Maria Giovanna De Pasquale, Claudia Ferlazzo, Guido Caccamo, Maria Teresa Magazù, Salvatore Guglielmino, Salvatore Pietro Paolo Gugliandolo, Concetta Microorganisms Article We report the ability of the crude biosurfactant (BS B3-15), produced by the marine, thermotolerant Bacillus licheniformis B3-15, to hinder the adhesion and biofilm formation of Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 29213 to polystyrene and human cells. First, we attempted to increase the BS yield, optimizing the culture conditions, and evaluated the surface-active properties of cell-free supernatants. Under phosphate deprivation (0.06 mM) and 5% saccharose, the yield of BS (1.5 g/L) increased by 37%, which could be explained by the earlier (12 h) increase in lchAA expression compared to the non-optimized condition (48 h). Without exerting any anti-bacterial activity, BS (300 µg/mL) prevented the adhesion of P. aeruginosa and S. aureus to polystyrene (47% and 36%, respectively) and disrupted the preformed biofilms, being more efficient against S. aureus (47%) than P. aeruginosa (26%). When added to human cells, the BS reduced the adhesion of P. aeruginosa and S. aureus (10× and 100,000× CFU/mL, respectively) without altering the epithelial cells’ viability. As it is not cytotoxic, BS B3-15 could be useful to prevent or remove bacterial biofilms in several medical and non-medical applications. MDPI 2023-07-20 /pmc/articles/PMC10384595/ /pubmed/37513014 http://dx.doi.org/10.3390/microorganisms11071842 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zammuto, Vincenzo
Rizzo, Maria Giovanna
De Pasquale, Claudia
Ferlazzo, Guido
Caccamo, Maria Teresa
Magazù, Salvatore
Guglielmino, Salvatore Pietro Paolo
Gugliandolo, Concetta
Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties
title Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties
title_full Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties
title_fullStr Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties
title_full_unstemmed Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties
title_short Lichenysin-like Polypeptide Production by Bacillus licheniformis B3-15 and Its Antiadhesive and Antibiofilm Properties
title_sort lichenysin-like polypeptide production by bacillus licheniformis b3-15 and its antiadhesive and antibiofilm properties
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384595/
https://www.ncbi.nlm.nih.gov/pubmed/37513014
http://dx.doi.org/10.3390/microorganisms11071842
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