Cargando…

Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)

IL-10 and IL-6 play important roles in protecting against inflammation and clearing pathogens from the body. In this study, homologous compounds of IL-10 and IL-6 were identified in channel catfish, and their immune responses were analyzed. The CDS sequences of IL-10 and IL-6 were 549 bp and 642 bp,...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Xia, Liu, Yongtao, Xu, Ning, Ai, Xiaohui, Yang, Yibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384647/
https://www.ncbi.nlm.nih.gov/pubmed/37513733
http://dx.doi.org/10.3390/pathogens12070886
_version_ 1785081208678383616
author Zhu, Xia
Liu, Yongtao
Xu, Ning
Ai, Xiaohui
Yang, Yibin
author_facet Zhu, Xia
Liu, Yongtao
Xu, Ning
Ai, Xiaohui
Yang, Yibin
author_sort Zhu, Xia
collection PubMed
description IL-10 and IL-6 play important roles in protecting against inflammation and clearing pathogens from the body. In this study, homologous compounds of IL-10 and IL-6 were identified in channel catfish, and their immune responses were analyzed. The CDS sequences of IL-10 and IL-6 were 549 bp and 642 bp, respectively, and showed the highest homology with Ameiurus melas. In addition, the expression of the IL-10 and IL-6 genes was ubiquitous in 10 tissues examined. IL-10 is highly expressed in the liver and slightly expressed in the gill. The high expression of the IL-6 gene was observed in the spleen, heart, and gonad, with the lowest levels in the liver. LPS, Poly(I:C), PHA, and PMA showed a highly significant increase in IL-10 and IL-6 expression 48 h after CCK stimulation (p < 0.01). Otherwise, Yersinia ruckeri, Streptococcus iniae, channel catfish virus, and deltamethrin induced IL-10 and IL-6 expression, varying in intensity between different organs. Our results suggest that IL-10 and IL-6 are involved in the immune response of the host against the pathogen.
format Online
Article
Text
id pubmed-10384647
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103846472023-07-30 Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus) Zhu, Xia Liu, Yongtao Xu, Ning Ai, Xiaohui Yang, Yibin Pathogens Article IL-10 and IL-6 play important roles in protecting against inflammation and clearing pathogens from the body. In this study, homologous compounds of IL-10 and IL-6 were identified in channel catfish, and their immune responses were analyzed. The CDS sequences of IL-10 and IL-6 were 549 bp and 642 bp, respectively, and showed the highest homology with Ameiurus melas. In addition, the expression of the IL-10 and IL-6 genes was ubiquitous in 10 tissues examined. IL-10 is highly expressed in the liver and slightly expressed in the gill. The high expression of the IL-6 gene was observed in the spleen, heart, and gonad, with the lowest levels in the liver. LPS, Poly(I:C), PHA, and PMA showed a highly significant increase in IL-10 and IL-6 expression 48 h after CCK stimulation (p < 0.01). Otherwise, Yersinia ruckeri, Streptococcus iniae, channel catfish virus, and deltamethrin induced IL-10 and IL-6 expression, varying in intensity between different organs. Our results suggest that IL-10 and IL-6 are involved in the immune response of the host against the pathogen. MDPI 2023-06-29 /pmc/articles/PMC10384647/ /pubmed/37513733 http://dx.doi.org/10.3390/pathogens12070886 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhu, Xia
Liu, Yongtao
Xu, Ning
Ai, Xiaohui
Yang, Yibin
Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)
title Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)
title_full Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)
title_fullStr Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)
title_full_unstemmed Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)
title_short Molecular Characterization and Expression Analysis of IL-10 and IL-6 in Channel Catfish (Ictalurus punctatus)
title_sort molecular characterization and expression analysis of il-10 and il-6 in channel catfish (ictalurus punctatus)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384647/
https://www.ncbi.nlm.nih.gov/pubmed/37513733
http://dx.doi.org/10.3390/pathogens12070886
work_keys_str_mv AT zhuxia molecularcharacterizationandexpressionanalysisofil10andil6inchannelcatfishictaluruspunctatus
AT liuyongtao molecularcharacterizationandexpressionanalysisofil10andil6inchannelcatfishictaluruspunctatus
AT xuning molecularcharacterizationandexpressionanalysisofil10andil6inchannelcatfishictaluruspunctatus
AT aixiaohui molecularcharacterizationandexpressionanalysisofil10andil6inchannelcatfishictaluruspunctatus
AT yangyibin molecularcharacterizationandexpressionanalysisofil10andil6inchannelcatfishictaluruspunctatus