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Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19

Brazil has the second-highest COVID-19 death rate worldwide, and Rio de Janeiro is among the states with the highest rate in the country. Although vaccine coverage has been achieved, it is anticipated that COVID-19 will transition into an endemic disease. It is concerning that the molecular mechanis...

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Autores principales: Gama-Almeida, Marcos C., Pinto, Gabriela D. A., Teixeira, Lívia, Hottz, Eugenio D., Ivens, Paula, Ribeiro, Hygor, Garrett, Rafael, Torres, Alexandre G., Carneiro, Talita I. A., Barbalho, Bianca de O., Ludwig, Christian, Struchiner, Claudio J., Assunção-Miranda, Iranaia, Valente, Ana Paula C., Bozza, Fernando A., Bozza, Patrícia T., dos Santos, Gilson C., El-Bacha, Tatiana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384698/
https://www.ncbi.nlm.nih.gov/pubmed/37512587
http://dx.doi.org/10.3390/metabo13070879
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author Gama-Almeida, Marcos C.
Pinto, Gabriela D. A.
Teixeira, Lívia
Hottz, Eugenio D.
Ivens, Paula
Ribeiro, Hygor
Garrett, Rafael
Torres, Alexandre G.
Carneiro, Talita I. A.
Barbalho, Bianca de O.
Ludwig, Christian
Struchiner, Claudio J.
Assunção-Miranda, Iranaia
Valente, Ana Paula C.
Bozza, Fernando A.
Bozza, Patrícia T.
dos Santos, Gilson C.
El-Bacha, Tatiana
author_facet Gama-Almeida, Marcos C.
Pinto, Gabriela D. A.
Teixeira, Lívia
Hottz, Eugenio D.
Ivens, Paula
Ribeiro, Hygor
Garrett, Rafael
Torres, Alexandre G.
Carneiro, Talita I. A.
Barbalho, Bianca de O.
Ludwig, Christian
Struchiner, Claudio J.
Assunção-Miranda, Iranaia
Valente, Ana Paula C.
Bozza, Fernando A.
Bozza, Patrícia T.
dos Santos, Gilson C.
El-Bacha, Tatiana
author_sort Gama-Almeida, Marcos C.
collection PubMed
description Brazil has the second-highest COVID-19 death rate worldwide, and Rio de Janeiro is among the states with the highest rate in the country. Although vaccine coverage has been achieved, it is anticipated that COVID-19 will transition into an endemic disease. It is concerning that the molecular mechanisms underlying clinical evolution from mild to severe disease, as well as the mechanisms leading to long COVID-19, are not yet fully understood. NMR and MS-based metabolomics were used to identify metabolites associated with COVID-19 pathophysiology and disease outcome. Severe COVID-19 cases (n = 35) were enrolled in two reference centers in Rio de Janeiro within 72 h of ICU admission, alongside 12 non-infected control subjects. COVID-19 patients were grouped into survivors (n = 18) and non-survivors (n = 17). Choline-related metabolites, serine, glycine, and betaine, were reduced in severe COVID-19, indicating dysregulation in methyl donors. Non-survivors had higher levels of creatine/creatinine, 4-hydroxyproline, gluconic acid, and N-acetylserine, indicating liver and kidney dysfunction. Several changes were greater in women; thus, patients’ sex should be considered in pandemic surveillance to achieve better disease stratification and improve outcomes. These metabolic alterations may be useful to monitor organ (dys) function and to understand the pathophysiology of acute and possibly post-acute COVID-19 syndromes.
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spelling pubmed-103846982023-07-30 Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19 Gama-Almeida, Marcos C. Pinto, Gabriela D. A. Teixeira, Lívia Hottz, Eugenio D. Ivens, Paula Ribeiro, Hygor Garrett, Rafael Torres, Alexandre G. Carneiro, Talita I. A. Barbalho, Bianca de O. Ludwig, Christian Struchiner, Claudio J. Assunção-Miranda, Iranaia Valente, Ana Paula C. Bozza, Fernando A. Bozza, Patrícia T. dos Santos, Gilson C. El-Bacha, Tatiana Metabolites Article Brazil has the second-highest COVID-19 death rate worldwide, and Rio de Janeiro is among the states with the highest rate in the country. Although vaccine coverage has been achieved, it is anticipated that COVID-19 will transition into an endemic disease. It is concerning that the molecular mechanisms underlying clinical evolution from mild to severe disease, as well as the mechanisms leading to long COVID-19, are not yet fully understood. NMR and MS-based metabolomics were used to identify metabolites associated with COVID-19 pathophysiology and disease outcome. Severe COVID-19 cases (n = 35) were enrolled in two reference centers in Rio de Janeiro within 72 h of ICU admission, alongside 12 non-infected control subjects. COVID-19 patients were grouped into survivors (n = 18) and non-survivors (n = 17). Choline-related metabolites, serine, glycine, and betaine, were reduced in severe COVID-19, indicating dysregulation in methyl donors. Non-survivors had higher levels of creatine/creatinine, 4-hydroxyproline, gluconic acid, and N-acetylserine, indicating liver and kidney dysfunction. Several changes were greater in women; thus, patients’ sex should be considered in pandemic surveillance to achieve better disease stratification and improve outcomes. These metabolic alterations may be useful to monitor organ (dys) function and to understand the pathophysiology of acute and possibly post-acute COVID-19 syndromes. MDPI 2023-07-24 /pmc/articles/PMC10384698/ /pubmed/37512587 http://dx.doi.org/10.3390/metabo13070879 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gama-Almeida, Marcos C.
Pinto, Gabriela D. A.
Teixeira, Lívia
Hottz, Eugenio D.
Ivens, Paula
Ribeiro, Hygor
Garrett, Rafael
Torres, Alexandre G.
Carneiro, Talita I. A.
Barbalho, Bianca de O.
Ludwig, Christian
Struchiner, Claudio J.
Assunção-Miranda, Iranaia
Valente, Ana Paula C.
Bozza, Fernando A.
Bozza, Patrícia T.
dos Santos, Gilson C.
El-Bacha, Tatiana
Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19
title Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19
title_full Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19
title_fullStr Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19
title_full_unstemmed Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19
title_short Integrated NMR and MS Analysis of the Plasma Metabolome Reveals Major Changes in One-Carbon, Lipid, and Amino Acid Metabolism in Severe and Fatal Cases of COVID-19
title_sort integrated nmr and ms analysis of the plasma metabolome reveals major changes in one-carbon, lipid, and amino acid metabolism in severe and fatal cases of covid-19
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384698/
https://www.ncbi.nlm.nih.gov/pubmed/37512587
http://dx.doi.org/10.3390/metabo13070879
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