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Detection of Mycobacterial DNA in Human Bone Marrow

Bone marrow is a cell-rich tissue of the reticuloendothelial system essential in the homeostasis and accurate functioning of hematopoiesis and of the immune system; moreover, it is also rich in lipids because it contains marrow adipocytes. This work aimed to evaluate the detection of mycobacterial D...

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Autores principales: González-Escalada, Alba, Rebollo, María José, Barrios Payan, Jorge, Hernández-Pando, Rogelio, García, María Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384717/
https://www.ncbi.nlm.nih.gov/pubmed/37512960
http://dx.doi.org/10.3390/microorganisms11071788
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author González-Escalada, Alba
Rebollo, María José
Barrios Payan, Jorge
Hernández-Pando, Rogelio
García, María Jesús
author_facet González-Escalada, Alba
Rebollo, María José
Barrios Payan, Jorge
Hernández-Pando, Rogelio
García, María Jesús
author_sort González-Escalada, Alba
collection PubMed
description Bone marrow is a cell-rich tissue of the reticuloendothelial system essential in the homeostasis and accurate functioning of hematopoiesis and of the immune system; moreover, it is also rich in lipids because it contains marrow adipocytes. This work aimed to evaluate the detection of mycobacterial DNA in human bone marrow as a tool to understand the complex pathology caused by the main pathogen Mycobacterium tuberculosis (Mtb). Formalin-fixed paraffin-embedded human bone marrow samples were studied using both conventional PCR + hybridization and in situ PCR to figure out the cell distribution of the targeted DNA. Samples were retrospectively collected from HIV+ patients with microbiologically proved mycobacterial infection and from subjects without evidence of infection. Mycobacterium avium (Mav) as well as Mtb DNA was detected in both settings, including tissues with and without granulomas. We detected DNA from both mycobacterial species, using in situ PCR, inside bone marrow macrophages. Other cell types, including adipocytes, showed positive signals only for Mtb DNA. This result suggested, for the first time, that marrow adipocytes could constitute an ideal reservoir for the persistence of Mtb, allowing the bacilli to establish long-lasting latent infection within a suitable lipid environment. This fact might differentiate pathogenic behavior of non-specialized pathogens such as Mav from that of specialized pathogens such as Mtb.
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spelling pubmed-103847172023-07-30 Detection of Mycobacterial DNA in Human Bone Marrow González-Escalada, Alba Rebollo, María José Barrios Payan, Jorge Hernández-Pando, Rogelio García, María Jesús Microorganisms Article Bone marrow is a cell-rich tissue of the reticuloendothelial system essential in the homeostasis and accurate functioning of hematopoiesis and of the immune system; moreover, it is also rich in lipids because it contains marrow adipocytes. This work aimed to evaluate the detection of mycobacterial DNA in human bone marrow as a tool to understand the complex pathology caused by the main pathogen Mycobacterium tuberculosis (Mtb). Formalin-fixed paraffin-embedded human bone marrow samples were studied using both conventional PCR + hybridization and in situ PCR to figure out the cell distribution of the targeted DNA. Samples were retrospectively collected from HIV+ patients with microbiologically proved mycobacterial infection and from subjects without evidence of infection. Mycobacterium avium (Mav) as well as Mtb DNA was detected in both settings, including tissues with and without granulomas. We detected DNA from both mycobacterial species, using in situ PCR, inside bone marrow macrophages. Other cell types, including adipocytes, showed positive signals only for Mtb DNA. This result suggested, for the first time, that marrow adipocytes could constitute an ideal reservoir for the persistence of Mtb, allowing the bacilli to establish long-lasting latent infection within a suitable lipid environment. This fact might differentiate pathogenic behavior of non-specialized pathogens such as Mav from that of specialized pathogens such as Mtb. MDPI 2023-07-11 /pmc/articles/PMC10384717/ /pubmed/37512960 http://dx.doi.org/10.3390/microorganisms11071788 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
González-Escalada, Alba
Rebollo, María José
Barrios Payan, Jorge
Hernández-Pando, Rogelio
García, María Jesús
Detection of Mycobacterial DNA in Human Bone Marrow
title Detection of Mycobacterial DNA in Human Bone Marrow
title_full Detection of Mycobacterial DNA in Human Bone Marrow
title_fullStr Detection of Mycobacterial DNA in Human Bone Marrow
title_full_unstemmed Detection of Mycobacterial DNA in Human Bone Marrow
title_short Detection of Mycobacterial DNA in Human Bone Marrow
title_sort detection of mycobacterial dna in human bone marrow
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384717/
https://www.ncbi.nlm.nih.gov/pubmed/37512960
http://dx.doi.org/10.3390/microorganisms11071788
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