Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials

The effect of primary amino acid sequence in recombinant spidroins on their spatial organization is crucial for the fabrication of artificial fibers and fibrous materials. This study focuses on the rheological properties of aqueous and alcoholic solutions of recombinant analogs of natural spidroins...

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Autores principales: Tenchurin, Timur Kh., Sharikov, Roman V., Belousov, Sergei I., Streltsov, Dmitry R., Malakhov, Sergey N., Yastremsky, Evgeny V., Chesnokov, Yuri M., Davydova, Lyubov I., Bogush, Vladimir G., Chvalun, Sergei N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384844/
https://www.ncbi.nlm.nih.gov/pubmed/37514391
http://dx.doi.org/10.3390/polym15143001
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author Tenchurin, Timur Kh.
Sharikov, Roman V.
Belousov, Sergei I.
Streltsov, Dmitry R.
Malakhov, Sergey N.
Yastremsky, Evgeny V.
Chesnokov, Yuri M.
Davydova, Lyubov I.
Bogush, Vladimir G.
Chvalun, Sergei N.
author_facet Tenchurin, Timur Kh.
Sharikov, Roman V.
Belousov, Sergei I.
Streltsov, Dmitry R.
Malakhov, Sergey N.
Yastremsky, Evgeny V.
Chesnokov, Yuri M.
Davydova, Lyubov I.
Bogush, Vladimir G.
Chvalun, Sergei N.
author_sort Tenchurin, Timur Kh.
collection PubMed
description The effect of primary amino acid sequence in recombinant spidroins on their spatial organization is crucial for the fabrication of artificial fibers and fibrous materials. This study focuses on the rheological properties of aqueous and alcoholic solutions of recombinant analogs of natural spidroins (rS1/9 and rS2/12), as well as the structure of their films and nanofibrous materials. Non-Newtonian flow behavior of aqueous solutions of these proteins was observed at certain concentrations in contrast to their solutions in hexafluoroisopropanol. The secondary structure of recombinant spidroins was addressed by IR spectroscopy, whereas their self-organization in various solvents was studied by AFM and cryo-TEM. The influence of the solvent on the structure and properties of the films and nanofibrous materials produced by electrospinning has been established.
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spelling pubmed-103848442023-07-30 Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials Tenchurin, Timur Kh. Sharikov, Roman V. Belousov, Sergei I. Streltsov, Dmitry R. Malakhov, Sergey N. Yastremsky, Evgeny V. Chesnokov, Yuri M. Davydova, Lyubov I. Bogush, Vladimir G. Chvalun, Sergei N. Polymers (Basel) Article The effect of primary amino acid sequence in recombinant spidroins on their spatial organization is crucial for the fabrication of artificial fibers and fibrous materials. This study focuses on the rheological properties of aqueous and alcoholic solutions of recombinant analogs of natural spidroins (rS1/9 and rS2/12), as well as the structure of their films and nanofibrous materials. Non-Newtonian flow behavior of aqueous solutions of these proteins was observed at certain concentrations in contrast to their solutions in hexafluoroisopropanol. The secondary structure of recombinant spidroins was addressed by IR spectroscopy, whereas their self-organization in various solvents was studied by AFM and cryo-TEM. The influence of the solvent on the structure and properties of the films and nanofibrous materials produced by electrospinning has been established. MDPI 2023-07-10 /pmc/articles/PMC10384844/ /pubmed/37514391 http://dx.doi.org/10.3390/polym15143001 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tenchurin, Timur Kh.
Sharikov, Roman V.
Belousov, Sergei I.
Streltsov, Dmitry R.
Malakhov, Sergey N.
Yastremsky, Evgeny V.
Chesnokov, Yuri M.
Davydova, Lyubov I.
Bogush, Vladimir G.
Chvalun, Sergei N.
Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials
title Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials
title_full Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials
title_fullStr Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials
title_full_unstemmed Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials
title_short Effect of Recombinant Spidroins Self-Assembly on Rheological Behavior of Their Dispersions and Structure of Electrospun Nanofibrous Materials
title_sort effect of recombinant spidroins self-assembly on rheological behavior of their dispersions and structure of electrospun nanofibrous materials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384844/
https://www.ncbi.nlm.nih.gov/pubmed/37514391
http://dx.doi.org/10.3390/polym15143001
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