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Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach

Trapezoidal cross-sectional spiral microfluidic channels showed high resolution and throughput in cell separation in bio-applications. The main challenges are the complexity and high cost of the fabrication process of trapezoidal cross-sectional channels on the micro-scale. In this work, we present...

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Autores principales: Adel, Mohamed, Allam, Ahmed, Sayour, Ashraf E., Ragai, Hani F., Umezu, Shinjiro, Fath El-Bab, Ahmed M. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384897/
https://www.ncbi.nlm.nih.gov/pubmed/37512651
http://dx.doi.org/10.3390/mi14071340
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author Adel, Mohamed
Allam, Ahmed
Sayour, Ashraf E.
Ragai, Hani F.
Umezu, Shinjiro
Fath El-Bab, Ahmed M. R.
author_facet Adel, Mohamed
Allam, Ahmed
Sayour, Ashraf E.
Ragai, Hani F.
Umezu, Shinjiro
Fath El-Bab, Ahmed M. R.
author_sort Adel, Mohamed
collection PubMed
description Trapezoidal cross-sectional spiral microfluidic channels showed high resolution and throughput in cell separation in bio-applications. The main challenges are the complexity and high cost of the fabrication process of trapezoidal cross-sectional channels on the micro-scale. In this work, we present the application of grayscale in microfluidic channel design to overcome the complexity of the fabrication process. We also use direct engraving with a CO(2) laser beam on polymethyl methacrylate (PMMA) material to drastically reduce the microfluidic chip’s cost (to <30 cents) and fabrication time (to 20 min). The capability of the present fabrication methodology for cell sorting applications is demonstrated through experimental tests for the separation of white blood cells (WBCs) from whole blood at different dilution factors. The experimental results indicated that an 800 µL/min flow rate provided the optimal separation efficiency using the fabricated chip. A 90.14% separation efficiency at 1% hematocrit diluted blood sample was reported.
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spelling pubmed-103848972023-07-30 Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach Adel, Mohamed Allam, Ahmed Sayour, Ashraf E. Ragai, Hani F. Umezu, Shinjiro Fath El-Bab, Ahmed M. R. Micromachines (Basel) Article Trapezoidal cross-sectional spiral microfluidic channels showed high resolution and throughput in cell separation in bio-applications. The main challenges are the complexity and high cost of the fabrication process of trapezoidal cross-sectional channels on the micro-scale. In this work, we present the application of grayscale in microfluidic channel design to overcome the complexity of the fabrication process. We also use direct engraving with a CO(2) laser beam on polymethyl methacrylate (PMMA) material to drastically reduce the microfluidic chip’s cost (to <30 cents) and fabrication time (to 20 min). The capability of the present fabrication methodology for cell sorting applications is demonstrated through experimental tests for the separation of white blood cells (WBCs) from whole blood at different dilution factors. The experimental results indicated that an 800 µL/min flow rate provided the optimal separation efficiency using the fabricated chip. A 90.14% separation efficiency at 1% hematocrit diluted blood sample was reported. MDPI 2023-06-30 /pmc/articles/PMC10384897/ /pubmed/37512651 http://dx.doi.org/10.3390/mi14071340 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Adel, Mohamed
Allam, Ahmed
Sayour, Ashraf E.
Ragai, Hani F.
Umezu, Shinjiro
Fath El-Bab, Ahmed M. R.
Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach
title Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach
title_full Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach
title_fullStr Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach
title_full_unstemmed Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach
title_short Fabrication of Spiral Low-Cost Microchannel with Trapezoidal Cross Section for Cell Separation Using a Grayscale Approach
title_sort fabrication of spiral low-cost microchannel with trapezoidal cross section for cell separation using a grayscale approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384897/
https://www.ncbi.nlm.nih.gov/pubmed/37512651
http://dx.doi.org/10.3390/mi14071340
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