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Effect of the Electric Field on the Biomineralization of Collagen
Collagen/hydroxyapatite hybrids are promising biomimetic materials that can replace or temporarily substitute bone tissues. The process of biomineralization was carried out through a double diffusion system. The methodological principle consisted in applying an electric field on the incubation mediu...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384922/ https://www.ncbi.nlm.nih.gov/pubmed/37514510 http://dx.doi.org/10.3390/polym15143121 |
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author | Ortiz, Fiorella Díaz-Barrios, Antonio Lopez-Cabaña, Zoraya E. González, Gema |
author_facet | Ortiz, Fiorella Díaz-Barrios, Antonio Lopez-Cabaña, Zoraya E. González, Gema |
author_sort | Ortiz, Fiorella |
collection | PubMed |
description | Collagen/hydroxyapatite hybrids are promising biomimetic materials that can replace or temporarily substitute bone tissues. The process of biomineralization was carried out through a double diffusion system. The methodological principle consisted in applying an electric field on the incubation medium to promote the opposite migration of ions into collagen membranes to form hydroxyapatite (HA) on the collagen membrane. Two physically separated solutions were used for the incubation medium, one rich in phosphate ions and the other in calcium ions, and their effects were evaluated against the traditional mineralization in Simulated Body Fluid (SBF). Pre-polarization of the organic membranes and the effect of incubation time on the biomineralization process were also assessed by FTIR and Raman spectroscopies.Our results demonstrated that the membrane pre-polarization significantly accelerated the mineralization process on collagen. On the other side, it was found that the application of the electric field influenced the collagen structure and its interactions with the mineral phase. The increment of the mineralization degree enhanced the photoluminescence properties of the collagen/HA materials, while the conductivity and the dielectric constant were reduced. These results might provide a useful approach for future applications in manufacturing biomimetic bone-like materials. |
format | Online Article Text |
id | pubmed-10384922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103849222023-07-30 Effect of the Electric Field on the Biomineralization of Collagen Ortiz, Fiorella Díaz-Barrios, Antonio Lopez-Cabaña, Zoraya E. González, Gema Polymers (Basel) Article Collagen/hydroxyapatite hybrids are promising biomimetic materials that can replace or temporarily substitute bone tissues. The process of biomineralization was carried out through a double diffusion system. The methodological principle consisted in applying an electric field on the incubation medium to promote the opposite migration of ions into collagen membranes to form hydroxyapatite (HA) on the collagen membrane. Two physically separated solutions were used for the incubation medium, one rich in phosphate ions and the other in calcium ions, and their effects were evaluated against the traditional mineralization in Simulated Body Fluid (SBF). Pre-polarization of the organic membranes and the effect of incubation time on the biomineralization process were also assessed by FTIR and Raman spectroscopies.Our results demonstrated that the membrane pre-polarization significantly accelerated the mineralization process on collagen. On the other side, it was found that the application of the electric field influenced the collagen structure and its interactions with the mineral phase. The increment of the mineralization degree enhanced the photoluminescence properties of the collagen/HA materials, while the conductivity and the dielectric constant were reduced. These results might provide a useful approach for future applications in manufacturing biomimetic bone-like materials. MDPI 2023-07-22 /pmc/articles/PMC10384922/ /pubmed/37514510 http://dx.doi.org/10.3390/polym15143121 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ortiz, Fiorella Díaz-Barrios, Antonio Lopez-Cabaña, Zoraya E. González, Gema Effect of the Electric Field on the Biomineralization of Collagen |
title | Effect of the Electric Field on the Biomineralization of Collagen |
title_full | Effect of the Electric Field on the Biomineralization of Collagen |
title_fullStr | Effect of the Electric Field on the Biomineralization of Collagen |
title_full_unstemmed | Effect of the Electric Field on the Biomineralization of Collagen |
title_short | Effect of the Electric Field on the Biomineralization of Collagen |
title_sort | effect of the electric field on the biomineralization of collagen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384922/ https://www.ncbi.nlm.nih.gov/pubmed/37514510 http://dx.doi.org/10.3390/polym15143121 |
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