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Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability

Titanium (Ti) is widely recognized for its exceptional properties and compatibility with medical applications. In our study, we successfully formed laser-induced periodic surface structures (LIPSS) on Ti plates with a periodicity of 520–740 nm and a height range of 150–250 nm. To investigate the mor...

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Autores principales: Grase, Liga, Onufrijevs, Pavels, Rezevska, Dace, Racenis, Karlis, Skadins, Ingus, Karosas, Jonas, Gecys, Paulius, Iesalnieks, Mairis, Pludons, Arturs, Kroica, Juta, Raciukaitis, Gediminas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384951/
https://www.ncbi.nlm.nih.gov/pubmed/37513043
http://dx.doi.org/10.3390/nano13142032
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author Grase, Liga
Onufrijevs, Pavels
Rezevska, Dace
Racenis, Karlis
Skadins, Ingus
Karosas, Jonas
Gecys, Paulius
Iesalnieks, Mairis
Pludons, Arturs
Kroica, Juta
Raciukaitis, Gediminas
author_facet Grase, Liga
Onufrijevs, Pavels
Rezevska, Dace
Racenis, Karlis
Skadins, Ingus
Karosas, Jonas
Gecys, Paulius
Iesalnieks, Mairis
Pludons, Arturs
Kroica, Juta
Raciukaitis, Gediminas
author_sort Grase, Liga
collection PubMed
description Titanium (Ti) is widely recognized for its exceptional properties and compatibility with medical applications. In our study, we successfully formed laser-induced periodic surface structures (LIPSS) on Ti plates with a periodicity of 520–740 nm and a height range of 150–250 nm. To investigate the morphology and chemical composition of these surfaces, we employed various techniques, including field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. Additionally, we utilized a drop-shape analyzer to determine the wetting properties of the surfaces. To evaluate the antibacterial activity, we followed the ISO 22196:2011 standard, utilizing reference bacterial cultures of Gram-positive Staphylococcus aureus (ATCC 25923) and Gram-negative Escherichia coli (ATCC 25922). The results revealed enhanced antibacterial properties against Staphylococcus aureus by more than 99% and Escherichia coli by more than 80% in comparison with non-irradiated Ti. Furthermore, we conducted experiments using the Escherichia coli bacteriophage T4 (ATCC 11303-B4) and the bacterial host Escherichia coli (ATCC 11303) to investigate the impact of Ti plates on the stability of the bacteriophage. Overall, our findings highlight the potential of LIPSS on Ti plates for achieving enhanced antibacterial activity against common bacterial strains while maintaining the stability of bacteriophages.
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spelling pubmed-103849512023-07-30 Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability Grase, Liga Onufrijevs, Pavels Rezevska, Dace Racenis, Karlis Skadins, Ingus Karosas, Jonas Gecys, Paulius Iesalnieks, Mairis Pludons, Arturs Kroica, Juta Raciukaitis, Gediminas Nanomaterials (Basel) Article Titanium (Ti) is widely recognized for its exceptional properties and compatibility with medical applications. In our study, we successfully formed laser-induced periodic surface structures (LIPSS) on Ti plates with a periodicity of 520–740 nm and a height range of 150–250 nm. To investigate the morphology and chemical composition of these surfaces, we employed various techniques, including field emission scanning electron microscopy, energy dispersive X-ray spectroscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy. Additionally, we utilized a drop-shape analyzer to determine the wetting properties of the surfaces. To evaluate the antibacterial activity, we followed the ISO 22196:2011 standard, utilizing reference bacterial cultures of Gram-positive Staphylococcus aureus (ATCC 25923) and Gram-negative Escherichia coli (ATCC 25922). The results revealed enhanced antibacterial properties against Staphylococcus aureus by more than 99% and Escherichia coli by more than 80% in comparison with non-irradiated Ti. Furthermore, we conducted experiments using the Escherichia coli bacteriophage T4 (ATCC 11303-B4) and the bacterial host Escherichia coli (ATCC 11303) to investigate the impact of Ti plates on the stability of the bacteriophage. Overall, our findings highlight the potential of LIPSS on Ti plates for achieving enhanced antibacterial activity against common bacterial strains while maintaining the stability of bacteriophages. MDPI 2023-07-09 /pmc/articles/PMC10384951/ /pubmed/37513043 http://dx.doi.org/10.3390/nano13142032 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grase, Liga
Onufrijevs, Pavels
Rezevska, Dace
Racenis, Karlis
Skadins, Ingus
Karosas, Jonas
Gecys, Paulius
Iesalnieks, Mairis
Pludons, Arturs
Kroica, Juta
Raciukaitis, Gediminas
Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability
title Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability
title_full Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability
title_fullStr Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability
title_full_unstemmed Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability
title_short Effect of Femtosecond Laser-Irradiated Titanium Plates on Enhanced Antibacterial Activity and Preservation of Bacteriophage Stability
title_sort effect of femtosecond laser-irradiated titanium plates on enhanced antibacterial activity and preservation of bacteriophage stability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10384951/
https://www.ncbi.nlm.nih.gov/pubmed/37513043
http://dx.doi.org/10.3390/nano13142032
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