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Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions

Adaptation ex vitro is strongly stressful for microplants. Plant-growth-promoting rhizobacteria (PGPR) help to increase the adaptation potential of microplants transplanted from test tubes into the natural environment. We investigated the mechanisms of antioxidant protection of PGPR-inoculated potat...

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Autores principales: Tkachenko, Oksana V., Evseeva, Nina V., Kargapolova, Kristina Y., Denisova, Alena Y., Pozdnyakova, Natalia N., Kulikov, Artem A., Burygin, Gennady L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385146/
https://www.ncbi.nlm.nih.gov/pubmed/37513038
http://dx.doi.org/10.3390/microorganisms11071866
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author Tkachenko, Oksana V.
Evseeva, Nina V.
Kargapolova, Kristina Y.
Denisova, Alena Y.
Pozdnyakova, Natalia N.
Kulikov, Artem A.
Burygin, Gennady L.
author_facet Tkachenko, Oksana V.
Evseeva, Nina V.
Kargapolova, Kristina Y.
Denisova, Alena Y.
Pozdnyakova, Natalia N.
Kulikov, Artem A.
Burygin, Gennady L.
author_sort Tkachenko, Oksana V.
collection PubMed
description Adaptation ex vitro is strongly stressful for microplants. Plant-growth-promoting rhizobacteria (PGPR) help to increase the adaptation potential of microplants transplanted from test tubes into the natural environment. We investigated the mechanisms of antioxidant protection of PGPR-inoculated potato microclones adapting to ex vitro growth in an aeroponic system. Potato (Solanum tuberosum L. cv. Nevsky) microplants were inoculated in vitro with the bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2. On days 1 and 7 of plant growth ex vitro, catalase and peroxidase activities in the leaves of inoculated plants were 1.5-fold higher than they were in non-inoculated plants. The activity of ascorbate peroxidase was reduced in both in vitro and ex vitro treatments, and this reduction was accompanied by a decrease in the leaf content of hydrogen peroxide and malondialdehyde. As a result, inoculation contributed to the regulation of the plant pro/antioxidant system, lowering the oxidative stress and leading to better plant survival ex vitro. This was evidenced by the higher values of measured morphological and physiological variables of the inoculated plants, as compared with the values in the control treatment. Thus, we have shown some PGPR-mediated mechanisms of potato plant protection from adverse environmental factors under aeroponic conditions.
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spelling pubmed-103851462023-07-30 Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions Tkachenko, Oksana V. Evseeva, Nina V. Kargapolova, Kristina Y. Denisova, Alena Y. Pozdnyakova, Natalia N. Kulikov, Artem A. Burygin, Gennady L. Microorganisms Article Adaptation ex vitro is strongly stressful for microplants. Plant-growth-promoting rhizobacteria (PGPR) help to increase the adaptation potential of microplants transplanted from test tubes into the natural environment. We investigated the mechanisms of antioxidant protection of PGPR-inoculated potato microclones adapting to ex vitro growth in an aeroponic system. Potato (Solanum tuberosum L. cv. Nevsky) microplants were inoculated in vitro with the bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2. On days 1 and 7 of plant growth ex vitro, catalase and peroxidase activities in the leaves of inoculated plants were 1.5-fold higher than they were in non-inoculated plants. The activity of ascorbate peroxidase was reduced in both in vitro and ex vitro treatments, and this reduction was accompanied by a decrease in the leaf content of hydrogen peroxide and malondialdehyde. As a result, inoculation contributed to the regulation of the plant pro/antioxidant system, lowering the oxidative stress and leading to better plant survival ex vitro. This was evidenced by the higher values of measured morphological and physiological variables of the inoculated plants, as compared with the values in the control treatment. Thus, we have shown some PGPR-mediated mechanisms of potato plant protection from adverse environmental factors under aeroponic conditions. MDPI 2023-07-24 /pmc/articles/PMC10385146/ /pubmed/37513038 http://dx.doi.org/10.3390/microorganisms11071866 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tkachenko, Oksana V.
Evseeva, Nina V.
Kargapolova, Kristina Y.
Denisova, Alena Y.
Pozdnyakova, Natalia N.
Kulikov, Artem A.
Burygin, Gennady L.
Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions
title Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions
title_full Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions
title_fullStr Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions
title_full_unstemmed Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions
title_short Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions
title_sort rhizobacteria increase the adaptation potential of potato microclones under aeroponic conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385146/
https://www.ncbi.nlm.nih.gov/pubmed/37513038
http://dx.doi.org/10.3390/microorganisms11071866
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