A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds

Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the A. baumannii-specific bacteriophages (B...

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Autores principales: Dehari, Deepa, Chaudhuri, Aiswarya, Kumar, Dulla Naveen, Patil, Rohit, Gangwar, Mayank, Rastogi, Sonam, Kumar, Dinesh, Nath, Gopal, Agrawal, Ashish Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385199/
https://www.ncbi.nlm.nih.gov/pubmed/37513854
http://dx.doi.org/10.3390/ph16070942
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author Dehari, Deepa
Chaudhuri, Aiswarya
Kumar, Dulla Naveen
Patil, Rohit
Gangwar, Mayank
Rastogi, Sonam
Kumar, Dinesh
Nath, Gopal
Agrawal, Ashish Kumar
author_facet Dehari, Deepa
Chaudhuri, Aiswarya
Kumar, Dulla Naveen
Patil, Rohit
Gangwar, Mayank
Rastogi, Sonam
Kumar, Dinesh
Nath, Gopal
Agrawal, Ashish Kumar
author_sort Dehari, Deepa
collection PubMed
description Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the A. baumannii-specific bacteriophages (BPABΦ1), loaded into the chitosan microparticles followed by dispersion in gel, and evaluated therapeutic efficacy against MDR A. baumannii clinical strains. Isolated BPABΦ1 were found to belong to the Corticoviridae family, with burst size 102.12 ± 2.65 PFUs per infected host cell. The BPABΦ1 loaded chitosan microparticles were evaluated for quality attributes viz. size, PDI, surface morphology, in vitro release, etc. The developed formulation exhibited excellent antibiofilm eradication potential in vitro and effective wound healing after topical application.
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spelling pubmed-103851992023-07-30 A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds Dehari, Deepa Chaudhuri, Aiswarya Kumar, Dulla Naveen Patil, Rohit Gangwar, Mayank Rastogi, Sonam Kumar, Dinesh Nath, Gopal Agrawal, Ashish Kumar Pharmaceuticals (Basel) Article Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is one of the major pathogens present in burn wound infections. Biofilm formation makes it further challenging to treat with clinically available antibiotics. In the current work, we isolated the A. baumannii-specific bacteriophages (BPABΦ1), loaded into the chitosan microparticles followed by dispersion in gel, and evaluated therapeutic efficacy against MDR A. baumannii clinical strains. Isolated BPABΦ1 were found to belong to the Corticoviridae family, with burst size 102.12 ± 2.65 PFUs per infected host cell. The BPABΦ1 loaded chitosan microparticles were evaluated for quality attributes viz. size, PDI, surface morphology, in vitro release, etc. The developed formulation exhibited excellent antibiofilm eradication potential in vitro and effective wound healing after topical application. MDPI 2023-06-29 /pmc/articles/PMC10385199/ /pubmed/37513854 http://dx.doi.org/10.3390/ph16070942 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dehari, Deepa
Chaudhuri, Aiswarya
Kumar, Dulla Naveen
Patil, Rohit
Gangwar, Mayank
Rastogi, Sonam
Kumar, Dinesh
Nath, Gopal
Agrawal, Ashish Kumar
A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds
title A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds
title_full A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds
title_fullStr A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds
title_full_unstemmed A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds
title_short A Bacteriophage Microgel Effectively Treats the Multidrug-Resistant Acinetobacter baumannii Bacterial Infections in Burn Wounds
title_sort bacteriophage microgel effectively treats the multidrug-resistant acinetobacter baumannii bacterial infections in burn wounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385199/
https://www.ncbi.nlm.nih.gov/pubmed/37513854
http://dx.doi.org/10.3390/ph16070942
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