Cargando…

The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)

The agronomic potential of glutamate dehydrogenase 2 (GDH2) in maize kernel production was investigated by examining the impact of a mutation on the corresponding gene. Mu-insertion homozygous and heterozygous mutant lines lacking GDH2 activity were isolated and characterized at the biochemical, phy...

Descripción completa

Detalles Bibliográficos
Autores principales: Tercé-Laforgue, Thérèse, Lothier, Jérémy, Limami, Anis M., Rouster, Jacques, Lea, Peter J., Hirel, Bertrand
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385319/
https://www.ncbi.nlm.nih.gov/pubmed/37514227
http://dx.doi.org/10.3390/plants12142612
_version_ 1785081376894091264
author Tercé-Laforgue, Thérèse
Lothier, Jérémy
Limami, Anis M.
Rouster, Jacques
Lea, Peter J.
Hirel, Bertrand
author_facet Tercé-Laforgue, Thérèse
Lothier, Jérémy
Limami, Anis M.
Rouster, Jacques
Lea, Peter J.
Hirel, Bertrand
author_sort Tercé-Laforgue, Thérèse
collection PubMed
description The agronomic potential of glutamate dehydrogenase 2 (GDH2) in maize kernel production was investigated by examining the impact of a mutation on the corresponding gene. Mu-insertion homozygous and heterozygous mutant lines lacking GDH2 activity were isolated and characterized at the biochemical, physiological and agronomic levels. In comparison to the wild type and to the homozygous ghd2 mutants, the heterozygous gdh2 mutant plants were characterized by a decrease in the root amino acid content, whereas in the leaves an increase of a number of phenolic compounds was observed. On average, a 30 to 40% increase in kernel yield was obtained only in the heterozygous gdh2 mutant lines when plants were grown in the field over two years. The importance of GDH2 in the control of plant productivity is discussed in relation to the physiological impact of the mutation on amino acid content, with primary carbon metabolism mostly occurring in the roots and secondary metabolism occurring in the leaves.
format Online
Article
Text
id pubmed-10385319
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103853192023-07-30 The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.) Tercé-Laforgue, Thérèse Lothier, Jérémy Limami, Anis M. Rouster, Jacques Lea, Peter J. Hirel, Bertrand Plants (Basel) Article The agronomic potential of glutamate dehydrogenase 2 (GDH2) in maize kernel production was investigated by examining the impact of a mutation on the corresponding gene. Mu-insertion homozygous and heterozygous mutant lines lacking GDH2 activity were isolated and characterized at the biochemical, physiological and agronomic levels. In comparison to the wild type and to the homozygous ghd2 mutants, the heterozygous gdh2 mutant plants were characterized by a decrease in the root amino acid content, whereas in the leaves an increase of a number of phenolic compounds was observed. On average, a 30 to 40% increase in kernel yield was obtained only in the heterozygous gdh2 mutant lines when plants were grown in the field over two years. The importance of GDH2 in the control of plant productivity is discussed in relation to the physiological impact of the mutation on amino acid content, with primary carbon metabolism mostly occurring in the roots and secondary metabolism occurring in the leaves. MDPI 2023-07-11 /pmc/articles/PMC10385319/ /pubmed/37514227 http://dx.doi.org/10.3390/plants12142612 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tercé-Laforgue, Thérèse
Lothier, Jérémy
Limami, Anis M.
Rouster, Jacques
Lea, Peter J.
Hirel, Bertrand
The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)
title The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)
title_full The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)
title_fullStr The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)
title_full_unstemmed The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)
title_short The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)
title_sort key role of glutamate dehydrogenase 2 (gdh2) in the control of kernel production in maize (zea mays l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385319/
https://www.ncbi.nlm.nih.gov/pubmed/37514227
http://dx.doi.org/10.3390/plants12142612
work_keys_str_mv AT tercelaforguetherese thekeyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT lothierjeremy thekeyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT limamianism thekeyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT rousterjacques thekeyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT leapeterj thekeyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT hirelbertrand thekeyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT tercelaforguetherese keyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT lothierjeremy keyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT limamianism keyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT rousterjacques keyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT leapeterj keyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl
AT hirelbertrand keyroleofglutamatedehydrogenase2gdh2inthecontrolofkernelproductioninmaizezeamaysl