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The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.)
The agronomic potential of glutamate dehydrogenase 2 (GDH2) in maize kernel production was investigated by examining the impact of a mutation on the corresponding gene. Mu-insertion homozygous and heterozygous mutant lines lacking GDH2 activity were isolated and characterized at the biochemical, phy...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385319/ https://www.ncbi.nlm.nih.gov/pubmed/37514227 http://dx.doi.org/10.3390/plants12142612 |
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author | Tercé-Laforgue, Thérèse Lothier, Jérémy Limami, Anis M. Rouster, Jacques Lea, Peter J. Hirel, Bertrand |
author_facet | Tercé-Laforgue, Thérèse Lothier, Jérémy Limami, Anis M. Rouster, Jacques Lea, Peter J. Hirel, Bertrand |
author_sort | Tercé-Laforgue, Thérèse |
collection | PubMed |
description | The agronomic potential of glutamate dehydrogenase 2 (GDH2) in maize kernel production was investigated by examining the impact of a mutation on the corresponding gene. Mu-insertion homozygous and heterozygous mutant lines lacking GDH2 activity were isolated and characterized at the biochemical, physiological and agronomic levels. In comparison to the wild type and to the homozygous ghd2 mutants, the heterozygous gdh2 mutant plants were characterized by a decrease in the root amino acid content, whereas in the leaves an increase of a number of phenolic compounds was observed. On average, a 30 to 40% increase in kernel yield was obtained only in the heterozygous gdh2 mutant lines when plants were grown in the field over two years. The importance of GDH2 in the control of plant productivity is discussed in relation to the physiological impact of the mutation on amino acid content, with primary carbon metabolism mostly occurring in the roots and secondary metabolism occurring in the leaves. |
format | Online Article Text |
id | pubmed-10385319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103853192023-07-30 The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.) Tercé-Laforgue, Thérèse Lothier, Jérémy Limami, Anis M. Rouster, Jacques Lea, Peter J. Hirel, Bertrand Plants (Basel) Article The agronomic potential of glutamate dehydrogenase 2 (GDH2) in maize kernel production was investigated by examining the impact of a mutation on the corresponding gene. Mu-insertion homozygous and heterozygous mutant lines lacking GDH2 activity were isolated and characterized at the biochemical, physiological and agronomic levels. In comparison to the wild type and to the homozygous ghd2 mutants, the heterozygous gdh2 mutant plants were characterized by a decrease in the root amino acid content, whereas in the leaves an increase of a number of phenolic compounds was observed. On average, a 30 to 40% increase in kernel yield was obtained only in the heterozygous gdh2 mutant lines when plants were grown in the field over two years. The importance of GDH2 in the control of plant productivity is discussed in relation to the physiological impact of the mutation on amino acid content, with primary carbon metabolism mostly occurring in the roots and secondary metabolism occurring in the leaves. MDPI 2023-07-11 /pmc/articles/PMC10385319/ /pubmed/37514227 http://dx.doi.org/10.3390/plants12142612 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tercé-Laforgue, Thérèse Lothier, Jérémy Limami, Anis M. Rouster, Jacques Lea, Peter J. Hirel, Bertrand The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.) |
title | The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.) |
title_full | The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.) |
title_fullStr | The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.) |
title_full_unstemmed | The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.) |
title_short | The Key Role of Glutamate Dehydrogenase 2 (GDH2) in the Control of Kernel Production in Maize (Zea mays L.) |
title_sort | key role of glutamate dehydrogenase 2 (gdh2) in the control of kernel production in maize (zea mays l.) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385319/ https://www.ncbi.nlm.nih.gov/pubmed/37514227 http://dx.doi.org/10.3390/plants12142612 |
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