Safety and Protective Efficacy of a Candidate Vector-Based Vaccine for Bovine Tuberculosis

This study presents the results of a survey of the safety and protective efficacy of a candidate vector-based vaccine for bovine tuberculosis, using an influenza vector with the NS1 mutation and expressing M. bovis protective antigens ESAT-6 and TB10.4. We vaccinated Balb/c outbred mice two times at...

Descripción completa

Detalles Bibliográficos
Autores principales: Abay, Zhandos, Nurpeisova, Ainur, Shorayeva, Kamshat, Sadikaliyeva, Sandugash, Yespembetov, Bolat, Syrym, Nazym, Sarmykova, Makhpal, Jekebekov, Kuanysh, Abitayev, Ruslan, Tokkarina, Gaukhar, Kalimolda, Elina, Absatova, Zharkinay, Moldagulova, Sabina, Yoo, Han Sang, Kassenov, Markhabat, Zakarya, Kunsulu, Abduraimov, Yergali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385609/
https://www.ncbi.nlm.nih.gov/pubmed/37515015
http://dx.doi.org/10.3390/vaccines11071199
Descripción
Sumario:This study presents the results of a survey of the safety and protective efficacy of a candidate vector-based vaccine for bovine tuberculosis, using an influenza vector with the NS1 mutation and expressing M. bovis protective antigens ESAT-6 and TB10.4. We vaccinated Balb/c outbred mice two times at 21 days apart. Our experimental design includes mice immunised with the candidate vaccine with or without adjuvant 15% Montanide Gel. The candidate vaccine’s safety was determined by biometric analysis, and protective efficacy was assessed by bacteriological and histological experiments following a virulent M. bovis-8 strain challenge. Our data indicated that the adjuvant-free version of the vaccine ensured complete protection from the M. bovis-8 infection in mice.

Ejemplares similares