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Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts

Over the past decades, Colombia has suffered complex social problems related to illicit crops, including forced displacement, violence, and environmental damage, among other consequences for vulnerable populations. Considerable effort has been made in the regulation of illicit crops, predominantly C...

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Autores principales: Camargo, Fidias D. González, Santamaria-Torres, Mary, Cala, Mónica P., Guevara-Suarez, Marcela, Restrepo, Silvia Restrepo, Sánchez-Camargo, Andrea, Fernández-Niño, Miguel, Corujo, María, Gallo Molina, Ada Carolina, Cifuentes, Javier, Serna, Julian A., Cruz, Juan C., Muñoz-Camargo, Carolina, Gonzalez Barrios, Andrés F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385671/
https://www.ncbi.nlm.nih.gov/pubmed/37512495
http://dx.doi.org/10.3390/metabo13070788
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author Camargo, Fidias D. González
Santamaria-Torres, Mary
Cala, Mónica P.
Guevara-Suarez, Marcela
Restrepo, Silvia Restrepo
Sánchez-Camargo, Andrea
Fernández-Niño, Miguel
Corujo, María
Gallo Molina, Ada Carolina
Cifuentes, Javier
Serna, Julian A.
Cruz, Juan C.
Muñoz-Camargo, Carolina
Gonzalez Barrios, Andrés F.
author_facet Camargo, Fidias D. González
Santamaria-Torres, Mary
Cala, Mónica P.
Guevara-Suarez, Marcela
Restrepo, Silvia Restrepo
Sánchez-Camargo, Andrea
Fernández-Niño, Miguel
Corujo, María
Gallo Molina, Ada Carolina
Cifuentes, Javier
Serna, Julian A.
Cruz, Juan C.
Muñoz-Camargo, Carolina
Gonzalez Barrios, Andrés F.
author_sort Camargo, Fidias D. González
collection PubMed
description Over the past decades, Colombia has suffered complex social problems related to illicit crops, including forced displacement, violence, and environmental damage, among other consequences for vulnerable populations. Considerable effort has been made in the regulation of illicit crops, predominantly Cannabis sativa, leading to advances such as the legalization of medical cannabis and its derivatives, the improvement of crops, and leaving an open window to the development of scientific knowledge to explore alternative uses. It is estimated that C. sativa can produce approximately 750 specialized secondary metabolites. Some of the most relevant due to their anticancer properties, besides cannabinoids, are monoterpenes, sesquiterpenoids, triterpenoids, essential oils, flavonoids, and phenolic compounds. However, despite the increase in scientific research on the subject, it is necessary to study the primary and secondary metabolism of the plant and to identify key pathways that explore its great metabolic potential. For this purpose, a genome-scale metabolic reconstruction of C. sativa is described and contextualized using LC-QTOF-MS metabolic data obtained from the leaf extract from plants grown in the region of Pesca-Boyaca, Colombia under greenhouse conditions at the Clever Leaves facility. A compartmentalized model with 2101 reactions and 1314 metabolites highlights pathways associated with fatty acid biosynthesis, steroids, and amino acids, along with the metabolism of purine, pyrimidine, glucose, starch, and sucrose. Key metabolites were identified through metabolomic data, such as neurine, cannabisativine, cannflavin A, palmitoleic acid, cannabinoids, geranylhydroquinone, and steroids. They were analyzed and integrated into the reconstruction, and their potential applications are discussed. Cytotoxicity assays revealed high anticancer activity against gastric adenocarcinoma (AGS), melanoma cells (A375), and lung carcinoma cells (A549), combined with negligible impact against healthy human skin cells.
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spelling pubmed-103856712023-07-30 Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts Camargo, Fidias D. González Santamaria-Torres, Mary Cala, Mónica P. Guevara-Suarez, Marcela Restrepo, Silvia Restrepo Sánchez-Camargo, Andrea Fernández-Niño, Miguel Corujo, María Gallo Molina, Ada Carolina Cifuentes, Javier Serna, Julian A. Cruz, Juan C. Muñoz-Camargo, Carolina Gonzalez Barrios, Andrés F. Metabolites Article Over the past decades, Colombia has suffered complex social problems related to illicit crops, including forced displacement, violence, and environmental damage, among other consequences for vulnerable populations. Considerable effort has been made in the regulation of illicit crops, predominantly Cannabis sativa, leading to advances such as the legalization of medical cannabis and its derivatives, the improvement of crops, and leaving an open window to the development of scientific knowledge to explore alternative uses. It is estimated that C. sativa can produce approximately 750 specialized secondary metabolites. Some of the most relevant due to their anticancer properties, besides cannabinoids, are monoterpenes, sesquiterpenoids, triterpenoids, essential oils, flavonoids, and phenolic compounds. However, despite the increase in scientific research on the subject, it is necessary to study the primary and secondary metabolism of the plant and to identify key pathways that explore its great metabolic potential. For this purpose, a genome-scale metabolic reconstruction of C. sativa is described and contextualized using LC-QTOF-MS metabolic data obtained from the leaf extract from plants grown in the region of Pesca-Boyaca, Colombia under greenhouse conditions at the Clever Leaves facility. A compartmentalized model with 2101 reactions and 1314 metabolites highlights pathways associated with fatty acid biosynthesis, steroids, and amino acids, along with the metabolism of purine, pyrimidine, glucose, starch, and sucrose. Key metabolites were identified through metabolomic data, such as neurine, cannabisativine, cannflavin A, palmitoleic acid, cannabinoids, geranylhydroquinone, and steroids. They were analyzed and integrated into the reconstruction, and their potential applications are discussed. Cytotoxicity assays revealed high anticancer activity against gastric adenocarcinoma (AGS), melanoma cells (A375), and lung carcinoma cells (A549), combined with negligible impact against healthy human skin cells. MDPI 2023-06-24 /pmc/articles/PMC10385671/ /pubmed/37512495 http://dx.doi.org/10.3390/metabo13070788 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Camargo, Fidias D. González
Santamaria-Torres, Mary
Cala, Mónica P.
Guevara-Suarez, Marcela
Restrepo, Silvia Restrepo
Sánchez-Camargo, Andrea
Fernández-Niño, Miguel
Corujo, María
Gallo Molina, Ada Carolina
Cifuentes, Javier
Serna, Julian A.
Cruz, Juan C.
Muñoz-Camargo, Carolina
Gonzalez Barrios, Andrés F.
Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts
title Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts
title_full Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts
title_fullStr Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts
title_full_unstemmed Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts
title_short Genome-Scale Metabolic Reconstruction, Non-Targeted LC-QTOF-MS Based Metabolomics Data, and Evaluation of Anticancer Activity of Cannabis sativa Leaf Extracts
title_sort genome-scale metabolic reconstruction, non-targeted lc-qtof-ms based metabolomics data, and evaluation of anticancer activity of cannabis sativa leaf extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10385671/
https://www.ncbi.nlm.nih.gov/pubmed/37512495
http://dx.doi.org/10.3390/metabo13070788
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