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Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis

In several low-income countries, the transport of sputa could take up to one week to reach the laboratories, resulting in increased contamination rates and a loss of growth. The aim of this study was to evaluate the effect of the OMNIgene-SPUTUM in preserving Mycobacterium tuberculosis on sputum sam...

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Autores principales: Mambuque, Edson, Saavedra, Belén, Molina-Moya, Barbara, Nguenha, Dinis, García-García, Esther, Blanco, Silvia, Gomes, Neide, Ehrlich, Joanna, Bulo, Helder, Munguambe, Shilzia, Chiconela, Helio, Acacio, Sozinho, Domínguez, José, García-Basteiro, Alberto L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386065/
https://www.ncbi.nlm.nih.gov/pubmed/37505663
http://dx.doi.org/10.3390/tropicalmed8070367
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author Mambuque, Edson
Saavedra, Belén
Molina-Moya, Barbara
Nguenha, Dinis
García-García, Esther
Blanco, Silvia
Gomes, Neide
Ehrlich, Joanna
Bulo, Helder
Munguambe, Shilzia
Chiconela, Helio
Acacio, Sozinho
Domínguez, José
García-Basteiro, Alberto L.
author_facet Mambuque, Edson
Saavedra, Belén
Molina-Moya, Barbara
Nguenha, Dinis
García-García, Esther
Blanco, Silvia
Gomes, Neide
Ehrlich, Joanna
Bulo, Helder
Munguambe, Shilzia
Chiconela, Helio
Acacio, Sozinho
Domínguez, José
García-Basteiro, Alberto L.
author_sort Mambuque, Edson
collection PubMed
description In several low-income countries, the transport of sputa could take up to one week to reach the laboratories, resulting in increased contamination rates and a loss of growth. The aim of this study was to evaluate the effect of the OMNIgene-SPUTUM in preserving Mycobacterium tuberculosis on sputum samples simulating three hypothetical scenarios for conservation and/or decontamination: (1) sputum was mixed with OMN and conserved at room temperature for five days and then processed for culture (OMN); (2) sputum cultures followed the routine standing operating procedure at day 0 (STD); and (3) sputum samples were kept at room temperature for five days and mixed with the standard decontamination reagent (SDT5) and then processed for culture. The positivity rate based on smear microscopy was 36.4%, 29.1%, and 27.3% for STD, STD5, and OMN, respectively. The proportion of positive results by liquid culture (MGIT) was 39.1% (43/110) for STD, 26.4% (29/110) for STD5, and 20.0% for OMN (22/110). The overall concordance of liquid culture results was 51.8% (57/110): 37.3% (41/110) for negative results, 11.8% (13/110) for MTBC growth, and 2.7% (3/110) for contaminated results. The OMN arm showed better performance in solid culture than in liquid culture, with a notable reduction in contaminated results.
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spelling pubmed-103860652023-07-30 Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis Mambuque, Edson Saavedra, Belén Molina-Moya, Barbara Nguenha, Dinis García-García, Esther Blanco, Silvia Gomes, Neide Ehrlich, Joanna Bulo, Helder Munguambe, Shilzia Chiconela, Helio Acacio, Sozinho Domínguez, José García-Basteiro, Alberto L. Trop Med Infect Dis Article In several low-income countries, the transport of sputa could take up to one week to reach the laboratories, resulting in increased contamination rates and a loss of growth. The aim of this study was to evaluate the effect of the OMNIgene-SPUTUM in preserving Mycobacterium tuberculosis on sputum samples simulating three hypothetical scenarios for conservation and/or decontamination: (1) sputum was mixed with OMN and conserved at room temperature for five days and then processed for culture (OMN); (2) sputum cultures followed the routine standing operating procedure at day 0 (STD); and (3) sputum samples were kept at room temperature for five days and mixed with the standard decontamination reagent (SDT5) and then processed for culture. The positivity rate based on smear microscopy was 36.4%, 29.1%, and 27.3% for STD, STD5, and OMN, respectively. The proportion of positive results by liquid culture (MGIT) was 39.1% (43/110) for STD, 26.4% (29/110) for STD5, and 20.0% for OMN (22/110). The overall concordance of liquid culture results was 51.8% (57/110): 37.3% (41/110) for negative results, 11.8% (13/110) for MTBC growth, and 2.7% (3/110) for contaminated results. The OMN arm showed better performance in solid culture than in liquid culture, with a notable reduction in contaminated results. MDPI 2023-07-17 /pmc/articles/PMC10386065/ /pubmed/37505663 http://dx.doi.org/10.3390/tropicalmed8070367 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mambuque, Edson
Saavedra, Belén
Molina-Moya, Barbara
Nguenha, Dinis
García-García, Esther
Blanco, Silvia
Gomes, Neide
Ehrlich, Joanna
Bulo, Helder
Munguambe, Shilzia
Chiconela, Helio
Acacio, Sozinho
Domínguez, José
García-Basteiro, Alberto L.
Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis
title Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis
title_full Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis
title_fullStr Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis
title_full_unstemmed Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis
title_short Evaluation of Omnigene-Sputum for Preservation of Sputum Samples for Diagnosis of Mycobacterium tuberculosis
title_sort evaluation of omnigene-sputum for preservation of sputum samples for diagnosis of mycobacterium tuberculosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386065/
https://www.ncbi.nlm.nih.gov/pubmed/37505663
http://dx.doi.org/10.3390/tropicalmed8070367
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