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Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas

Brassica species show varying levels of resistance to salt stress. To understand the genetics underlying these differential stress tolerance patterns in Brassicas, we exposed two widely cultivated amphidiploid Brassica species having different genomes, Brassica juncea (AABB, n = 18) and Brassica nap...

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Autores principales: Farooq, Nisma, Khan, Muhammad Omar, Ahmed, Muhammad Zaheer, Fatima, Samia, Nawaz, Muhammad Asif, Abideen, Zainul, Nielsen, Brent L., Ahmad, Niaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386101/
https://www.ncbi.nlm.nih.gov/pubmed/37514204
http://dx.doi.org/10.3390/plants12142590
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author Farooq, Nisma
Khan, Muhammad Omar
Ahmed, Muhammad Zaheer
Fatima, Samia
Nawaz, Muhammad Asif
Abideen, Zainul
Nielsen, Brent L.
Ahmad, Niaz
author_facet Farooq, Nisma
Khan, Muhammad Omar
Ahmed, Muhammad Zaheer
Fatima, Samia
Nawaz, Muhammad Asif
Abideen, Zainul
Nielsen, Brent L.
Ahmad, Niaz
author_sort Farooq, Nisma
collection PubMed
description Brassica species show varying levels of resistance to salt stress. To understand the genetics underlying these differential stress tolerance patterns in Brassicas, we exposed two widely cultivated amphidiploid Brassica species having different genomes, Brassica juncea (AABB, n = 18) and Brassica napus (AACC, n = 19), to elevated levels of NaCl concentration (300 mM, half the salinity of seawater). B. juncea produced more biomass, an increased chlorophyll content, and fewer accumulated sodium (Na(+)) and chloride (Cl(−)) ions in its photosynthesizing tissues. Chlorophyll fluorescence assays revealed that the reaction centers of PSII of B. juncea were more photoprotected and hence more active than those of B. napus under NaCl stress, which, in turn, resulted in a better PSII quantum efficiency, better utilization of photochemical energy with significantly reduced energy loss, and higher electron transport rates, even under stressful conditions. The expression of key genes responsible for salt tolerance (NHX1 and AVP1, which are nuclear-encoded) and photosynthesis (psbA, psaA, petB, and rbcL, which are chloroplast-encoded) were monitored for their genetic differences underlying stress tolerance. Under NaCl stress, the expression of NHX1, D1, and Rubisco increased several folds in B. juncea plants compared to B. napus, highlighting differences in genetics between these two Brassicas. The higher photosynthetic potential under stress suggests that B. juncea is a promising candidate for genetic modifications and its cultivation on marginal lands.
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spelling pubmed-103861012023-07-30 Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas Farooq, Nisma Khan, Muhammad Omar Ahmed, Muhammad Zaheer Fatima, Samia Nawaz, Muhammad Asif Abideen, Zainul Nielsen, Brent L. Ahmad, Niaz Plants (Basel) Article Brassica species show varying levels of resistance to salt stress. To understand the genetics underlying these differential stress tolerance patterns in Brassicas, we exposed two widely cultivated amphidiploid Brassica species having different genomes, Brassica juncea (AABB, n = 18) and Brassica napus (AACC, n = 19), to elevated levels of NaCl concentration (300 mM, half the salinity of seawater). B. juncea produced more biomass, an increased chlorophyll content, and fewer accumulated sodium (Na(+)) and chloride (Cl(−)) ions in its photosynthesizing tissues. Chlorophyll fluorescence assays revealed that the reaction centers of PSII of B. juncea were more photoprotected and hence more active than those of B. napus under NaCl stress, which, in turn, resulted in a better PSII quantum efficiency, better utilization of photochemical energy with significantly reduced energy loss, and higher electron transport rates, even under stressful conditions. The expression of key genes responsible for salt tolerance (NHX1 and AVP1, which are nuclear-encoded) and photosynthesis (psbA, psaA, petB, and rbcL, which are chloroplast-encoded) were monitored for their genetic differences underlying stress tolerance. Under NaCl stress, the expression of NHX1, D1, and Rubisco increased several folds in B. juncea plants compared to B. napus, highlighting differences in genetics between these two Brassicas. The higher photosynthetic potential under stress suggests that B. juncea is a promising candidate for genetic modifications and its cultivation on marginal lands. MDPI 2023-07-08 /pmc/articles/PMC10386101/ /pubmed/37514204 http://dx.doi.org/10.3390/plants12142590 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Farooq, Nisma
Khan, Muhammad Omar
Ahmed, Muhammad Zaheer
Fatima, Samia
Nawaz, Muhammad Asif
Abideen, Zainul
Nielsen, Brent L.
Ahmad, Niaz
Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas
title Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas
title_full Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas
title_fullStr Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas
title_full_unstemmed Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas
title_short Salt-Induced Modulation of Ion Transport and PSII Photoprotection Determine the Salinity Tolerance of Amphidiploid Brassicas
title_sort salt-induced modulation of ion transport and psii photoprotection determine the salinity tolerance of amphidiploid brassicas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386101/
https://www.ncbi.nlm.nih.gov/pubmed/37514204
http://dx.doi.org/10.3390/plants12142590
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