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Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing

We selected a novel biotin-binding peptide for sensing biotin, biotinylated proteins, and nucleotides. From a 15-mer library displayed on the RNA coliphage Qβ, a 15-amino acid long peptide (HGHGWQIPVWPWGQG) hereby referred to as a nanotag was identified to selectively bind biotin. The target selecti...

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Autores principales: Ntemafack, Augustin, Dzelamonyuy, Aristide, Nchinda, Godwin, Bopda Waffo, Alain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386108/
https://www.ncbi.nlm.nih.gov/pubmed/37515102
http://dx.doi.org/10.3390/v15071414
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author Ntemafack, Augustin
Dzelamonyuy, Aristide
Nchinda, Godwin
Bopda Waffo, Alain
author_facet Ntemafack, Augustin
Dzelamonyuy, Aristide
Nchinda, Godwin
Bopda Waffo, Alain
author_sort Ntemafack, Augustin
collection PubMed
description We selected a novel biotin-binding peptide for sensing biotin, biotinylated proteins, and nucleotides. From a 15-mer library displayed on the RNA coliphage Qβ, a 15-amino acid long peptide (HGHGWQIPVWPWGQG) hereby referred to as a nanotag was identified to selectively bind biotin. The target selection was achieved through panning with elution by infection. The selected peptide was tested as a transducer for an immunogenic epitope of the foot-and-mouth disease virus (FMDV) on Qβ phage platform separated by a linker. The biotin-tag showed no significant influence on the affinity of the epitope to its cognate antibody (SD6). The nanotag-bound biotin selectively fused either to the C- or N-terminus of the epitope. The epitope would not bind or recognize SD6 while positioned at the N-terminus of the nanotag. Additionally, the biotin competed linearly with the SD6 antibody in a competitive ELISA. Competition assays using the selected recombinant phage itself as a probe or transducer enable the operationalization of this technology as a biosensor toolkit to sense and quantify SD6 analyte. Herein, the published Strep II nanotag (DVEWLDERVPLVET) was used as a control and has similar functionalities to our proposed novel biotin-tag thereby providing a new platform for developing devices for diagnostic purposes.
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spelling pubmed-103861082023-07-30 Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing Ntemafack, Augustin Dzelamonyuy, Aristide Nchinda, Godwin Bopda Waffo, Alain Viruses Article We selected a novel biotin-binding peptide for sensing biotin, biotinylated proteins, and nucleotides. From a 15-mer library displayed on the RNA coliphage Qβ, a 15-amino acid long peptide (HGHGWQIPVWPWGQG) hereby referred to as a nanotag was identified to selectively bind biotin. The target selection was achieved through panning with elution by infection. The selected peptide was tested as a transducer for an immunogenic epitope of the foot-and-mouth disease virus (FMDV) on Qβ phage platform separated by a linker. The biotin-tag showed no significant influence on the affinity of the epitope to its cognate antibody (SD6). The nanotag-bound biotin selectively fused either to the C- or N-terminus of the epitope. The epitope would not bind or recognize SD6 while positioned at the N-terminus of the nanotag. Additionally, the biotin competed linearly with the SD6 antibody in a competitive ELISA. Competition assays using the selected recombinant phage itself as a probe or transducer enable the operationalization of this technology as a biosensor toolkit to sense and quantify SD6 analyte. Herein, the published Strep II nanotag (DVEWLDERVPLVET) was used as a control and has similar functionalities to our proposed novel biotin-tag thereby providing a new platform for developing devices for diagnostic purposes. MDPI 2023-06-22 /pmc/articles/PMC10386108/ /pubmed/37515102 http://dx.doi.org/10.3390/v15071414 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ntemafack, Augustin
Dzelamonyuy, Aristide
Nchinda, Godwin
Bopda Waffo, Alain
Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing
title Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing
title_full Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing
title_fullStr Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing
title_full_unstemmed Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing
title_short Evolutionary Qβ Phage Displayed Nanotag Library and Peptides for Biosensing
title_sort evolutionary qβ phage displayed nanotag library and peptides for biosensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386108/
https://www.ncbi.nlm.nih.gov/pubmed/37515102
http://dx.doi.org/10.3390/v15071414
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