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A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS)
Sperm motility analysis of aquatic model species is important yet challenging due to the small sample volume, the necessity to activate with water, and the short duration of motility. To achieve standardization of sperm activation, microfluidic mixers have shown improved reproducibility over activat...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386169/ https://www.ncbi.nlm.nih.gov/pubmed/37512621 http://dx.doi.org/10.3390/mi14071310 |
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author | Belgodere, Jorge A. Alam, Mustafa Browning, Valentino E. Eades, Jason North, Jack Armand, Julie A. Liu, Yue Tiersch, Terrence R. Monroe, W. Todd |
author_facet | Belgodere, Jorge A. Alam, Mustafa Browning, Valentino E. Eades, Jason North, Jack Armand, Julie A. Liu, Yue Tiersch, Terrence R. Monroe, W. Todd |
author_sort | Belgodere, Jorge A. |
collection | PubMed |
description | Sperm motility analysis of aquatic model species is important yet challenging due to the small sample volume, the necessity to activate with water, and the short duration of motility. To achieve standardization of sperm activation, microfluidic mixers have shown improved reproducibility over activation by hand, but challenges remain in optimizing and simplifying the use of these microdevices for greater adoption. The device described herein incorporates a novel micromixer geometry that aligns two sperm inlet streams with modified herringbone structures that split and recombine the sample at a 1:6 dilution with water to achieve rapid and consistent initiation of motility. The polydimethylsiloxane (PDMS) chip can be operated in a positive or negative pressure configuration, allowing a simple micropipettor to draw samples into the chip and rapidly stop the flow. The device was optimized to not only activate zebrafish sperm but also enables practical use with standard computer-assisted sperm analysis (CASA) systems. The micromixer geometry could be modified for other aquatic species with differing cell sizes and adopted for an open hardware approach using 3D resin printing where users could revise, fabricate, and share designs to improve standardization and reproducibility across laboratories and repositories. |
format | Online Article Text |
id | pubmed-10386169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103861692023-07-30 A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS) Belgodere, Jorge A. Alam, Mustafa Browning, Valentino E. Eades, Jason North, Jack Armand, Julie A. Liu, Yue Tiersch, Terrence R. Monroe, W. Todd Micromachines (Basel) Article Sperm motility analysis of aquatic model species is important yet challenging due to the small sample volume, the necessity to activate with water, and the short duration of motility. To achieve standardization of sperm activation, microfluidic mixers have shown improved reproducibility over activation by hand, but challenges remain in optimizing and simplifying the use of these microdevices for greater adoption. The device described herein incorporates a novel micromixer geometry that aligns two sperm inlet streams with modified herringbone structures that split and recombine the sample at a 1:6 dilution with water to achieve rapid and consistent initiation of motility. The polydimethylsiloxane (PDMS) chip can be operated in a positive or negative pressure configuration, allowing a simple micropipettor to draw samples into the chip and rapidly stop the flow. The device was optimized to not only activate zebrafish sperm but also enables practical use with standard computer-assisted sperm analysis (CASA) systems. The micromixer geometry could be modified for other aquatic species with differing cell sizes and adopted for an open hardware approach using 3D resin printing where users could revise, fabricate, and share designs to improve standardization and reproducibility across laboratories and repositories. MDPI 2023-06-26 /pmc/articles/PMC10386169/ /pubmed/37512621 http://dx.doi.org/10.3390/mi14071310 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Belgodere, Jorge A. Alam, Mustafa Browning, Valentino E. Eades, Jason North, Jack Armand, Julie A. Liu, Yue Tiersch, Terrence R. Monroe, W. Todd A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS) |
title | A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS) |
title_full | A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS) |
title_fullStr | A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS) |
title_full_unstemmed | A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS) |
title_short | A Modified-Herringbone Micromixer for Assessing Zebrafish Sperm (MAGS) |
title_sort | modified-herringbone micromixer for assessing zebrafish sperm (mags) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386169/ https://www.ncbi.nlm.nih.gov/pubmed/37512621 http://dx.doi.org/10.3390/mi14071310 |
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