Cargando…
Occurrence and Patterns of Enterotoxin Genes, spa Types and Antimicrobial Resistance Patterns in Staphylococcus aureus in Food and Food Contact Surfaces in Singapore
Staphylococcus aureus contamination of food and food contact surfaces is a public health concern given its virulent and antimicrobial-resistant properties worldwide. In this study, a total of 181 MSSA isolates were analyzed for SE genes, antimicrobial resistance patterns, and spa types. Overall, 24....
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386305/ https://www.ncbi.nlm.nih.gov/pubmed/37512957 http://dx.doi.org/10.3390/microorganisms11071785 |
_version_ | 1785081631442206720 |
---|---|
author | Lim, Ker Li Khor, Wei Ching Ong, Kar Hui Timothy, Lois Aung, Kyaw Thu |
author_facet | Lim, Ker Li Khor, Wei Ching Ong, Kar Hui Timothy, Lois Aung, Kyaw Thu |
author_sort | Lim, Ker Li |
collection | PubMed |
description | Staphylococcus aureus contamination of food and food contact surfaces is a public health concern given its virulent and antimicrobial-resistant properties worldwide. In this study, a total of 181 MSSA isolates were analyzed for SE genes, antimicrobial resistance patterns, and spa types. Overall, 24.9% of isolates were positive for SE gene detection, with sea being the most prevalent classical SE (18.8%). The most predominant sample sources for SE gene contamination were hand swabs for sea (6/48), meat dishes for seb (3/14) and seafood dishes for sec (2/24). Antimicrobial resistance was also observed at relatively high frequencies for the clinically important antibiotics penicillin G and ampicillin (both 54.7%), followed by tetracycline (14.9%) and azithromycin (8.8%). In addition, characterization of spa types revealed spa type t5078 to be the most predominant (40.3%), with significant associations between spa types t127 and t5521 and the sea gene. This study offers insights into the enterotoxin gene and antimicrobial resistance profiles of S. aureus in cooked or ready-to-eat food to inform future surveillance and epidemiological studies. |
format | Online Article Text |
id | pubmed-10386305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-103863052023-07-30 Occurrence and Patterns of Enterotoxin Genes, spa Types and Antimicrobial Resistance Patterns in Staphylococcus aureus in Food and Food Contact Surfaces in Singapore Lim, Ker Li Khor, Wei Ching Ong, Kar Hui Timothy, Lois Aung, Kyaw Thu Microorganisms Article Staphylococcus aureus contamination of food and food contact surfaces is a public health concern given its virulent and antimicrobial-resistant properties worldwide. In this study, a total of 181 MSSA isolates were analyzed for SE genes, antimicrobial resistance patterns, and spa types. Overall, 24.9% of isolates were positive for SE gene detection, with sea being the most prevalent classical SE (18.8%). The most predominant sample sources for SE gene contamination were hand swabs for sea (6/48), meat dishes for seb (3/14) and seafood dishes for sec (2/24). Antimicrobial resistance was also observed at relatively high frequencies for the clinically important antibiotics penicillin G and ampicillin (both 54.7%), followed by tetracycline (14.9%) and azithromycin (8.8%). In addition, characterization of spa types revealed spa type t5078 to be the most predominant (40.3%), with significant associations between spa types t127 and t5521 and the sea gene. This study offers insights into the enterotoxin gene and antimicrobial resistance profiles of S. aureus in cooked or ready-to-eat food to inform future surveillance and epidemiological studies. MDPI 2023-07-10 /pmc/articles/PMC10386305/ /pubmed/37512957 http://dx.doi.org/10.3390/microorganisms11071785 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lim, Ker Li Khor, Wei Ching Ong, Kar Hui Timothy, Lois Aung, Kyaw Thu Occurrence and Patterns of Enterotoxin Genes, spa Types and Antimicrobial Resistance Patterns in Staphylococcus aureus in Food and Food Contact Surfaces in Singapore |
title | Occurrence and Patterns of Enterotoxin Genes, spa Types and Antimicrobial Resistance Patterns in Staphylococcus aureus in Food and Food Contact Surfaces in Singapore |
title_full | Occurrence and Patterns of Enterotoxin Genes, spa Types and Antimicrobial Resistance Patterns in Staphylococcus aureus in Food and Food Contact Surfaces in Singapore |
title_fullStr | Occurrence and Patterns of Enterotoxin Genes, spa Types and Antimicrobial Resistance Patterns in Staphylococcus aureus in Food and Food Contact Surfaces in Singapore |
title_full_unstemmed | Occurrence and Patterns of Enterotoxin Genes, spa Types and Antimicrobial Resistance Patterns in Staphylococcus aureus in Food and Food Contact Surfaces in Singapore |
title_short | Occurrence and Patterns of Enterotoxin Genes, spa Types and Antimicrobial Resistance Patterns in Staphylococcus aureus in Food and Food Contact Surfaces in Singapore |
title_sort | occurrence and patterns of enterotoxin genes, spa types and antimicrobial resistance patterns in staphylococcus aureus in food and food contact surfaces in singapore |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386305/ https://www.ncbi.nlm.nih.gov/pubmed/37512957 http://dx.doi.org/10.3390/microorganisms11071785 |
work_keys_str_mv | AT limkerli occurrenceandpatternsofenterotoxingenesspatypesandantimicrobialresistancepatternsinstaphylococcusaureusinfoodandfoodcontactsurfacesinsingapore AT khorweiching occurrenceandpatternsofenterotoxingenesspatypesandantimicrobialresistancepatternsinstaphylococcusaureusinfoodandfoodcontactsurfacesinsingapore AT ongkarhui occurrenceandpatternsofenterotoxingenesspatypesandantimicrobialresistancepatternsinstaphylococcusaureusinfoodandfoodcontactsurfacesinsingapore AT timothylois occurrenceandpatternsofenterotoxingenesspatypesandantimicrobialresistancepatternsinstaphylococcusaureusinfoodandfoodcontactsurfacesinsingapore AT aungkyawthu occurrenceandpatternsofenterotoxingenesspatypesandantimicrobialresistancepatternsinstaphylococcusaureusinfoodandfoodcontactsurfacesinsingapore |