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Blood metabolomics reveals the therapeutic effect of Pueraria polysaccharide on calf diarrhea

BACKGROUND: Neonatal calf diarrhea (NCD) is typically treated with antibiotics, while long-term application of antibiotics induces drug resistance and antibiotic residues, ultimately decreasing feed efficiency. Pueraria polysaccharide (PPL) is a versatile antimicrobial, immunomodulatory, and antioxi...

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Detalles Bibliográficos
Autores principales: Shen, Liuhong, Shen, Yu, You, Liuchao, Zhang, Yue, Su, Zhetong, Peng, Guangneng, Deng, Jun-liang, Zhong, Zhijun, Yu, Shumin, Zong, Xiaolan, Wu, Xiaofeng, Zhu, Yingkun, Cao, Suizhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386334/
https://www.ncbi.nlm.nih.gov/pubmed/37516856
http://dx.doi.org/10.1186/s12917-023-03662-9
Descripción
Sumario:BACKGROUND: Neonatal calf diarrhea (NCD) is typically treated with antibiotics, while long-term application of antibiotics induces drug resistance and antibiotic residues, ultimately decreasing feed efficiency. Pueraria polysaccharide (PPL) is a versatile antimicrobial, immunomodulatory, and antioxidative compound. This study aimed to compare the therapeutic efficacy of different doses of PPL (0.2, 0.4, 0.8 g/kg body weight (BW)) and explore the effect of plasma metabolites in diarrheal calves by the best dose of PPL. RESULTS: PPL could effectively improve the daily weight gain, fecal score, and dehydration score, and the dosage of 0.4 g/kg BW could reach curative efficacy against calf diarrhea (with effective rates 100.00%). Metabolomic analysis suggested that diarrhea mainly affect the levels of taurocholate, DL-lactate, LysoPCs, and intestinal flora-related metabolites, trimethylamine N-oxide; however, PPL improved liver function and intestinal barrier integrity by modulating the levels of DL-lactate, LysoPC (18:0/0:0) and bilirubin, which eventually attenuated neonatal calf diarrhea. It also suggested that the therapeutic effect of PPL is related to those differential metabolites in diarrheal calves. CONCLUSIONS: The results showed that 0.4 g/kg BW PPL could restore the clinical score of diarrhea calves by improving the blood indexes, biochemical indexes, and blood metabolites. And it is a potential medicine for the treatment of calf diarrhea. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-023-03662-9.