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Comparative Analysis of the Exo-Erythrocytic Development of Five Lineages of Haemoproteus majoris, a Common Haemosporidian Parasite of European Passeriform Birds

Haemoproteus parasites (Apicomplexa, Haemosporida) are widespread pathogens of birds, with a rich genetic (about 1900 lineages) and morphospecies (178 species) diversity. Nonetheless, their life cycles are poorly understood. The exo-erythrocytic stages of three Haemoproteus majoris (widespread gener...

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Autores principales: Duc, Mélanie, Himmel, Tanja, Harl, Josef, Iezhova, Tatjana, Nedorost, Nora, Matt, Julia, Ilgūnas, Mikas, Weissenböck, Herbert, Valkiūnas, Gediminas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386383/
https://www.ncbi.nlm.nih.gov/pubmed/37513745
http://dx.doi.org/10.3390/pathogens12070898
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author Duc, Mélanie
Himmel, Tanja
Harl, Josef
Iezhova, Tatjana
Nedorost, Nora
Matt, Julia
Ilgūnas, Mikas
Weissenböck, Herbert
Valkiūnas, Gediminas
author_facet Duc, Mélanie
Himmel, Tanja
Harl, Josef
Iezhova, Tatjana
Nedorost, Nora
Matt, Julia
Ilgūnas, Mikas
Weissenböck, Herbert
Valkiūnas, Gediminas
author_sort Duc, Mélanie
collection PubMed
description Haemoproteus parasites (Apicomplexa, Haemosporida) are widespread pathogens of birds, with a rich genetic (about 1900 lineages) and morphospecies (178 species) diversity. Nonetheless, their life cycles are poorly understood. The exo-erythrocytic stages of three Haemoproteus majoris (widespread generalist parasite) lineages have been previously reported, each in a different bird species. We aimed to further study and compare the development of five H. majoris lineages—hCCF5, hCWT4, hPARUS1, hPHSIB1, and hWW2—in a wider selection of natural avian hosts. A total of 42 individuals belonging to 14 bird species were sampled. Morphospecies and parasitemia were determined by microscopy of blood films, lineages by DNA-barcoding a 478 bp section of the cytochrome b gene, and exo-erythrocytic stages by histology and chromogenic in situ hybridization. The lineage hCWT4 was morphologically characterized as H. majoris for the first time. All lineage infections exclusively featured megalomeronts. The exo-erythrocytic stages found in all examined bird species were similar, particularly for the lineages hCCF5, hPARUS1, and hPHSIB1. Megalomeronts of the lineages hWW2 and hCWT4 were more similar to each other than to the former three lineages. The kidneys and gizzard were most often affected, followed by lungs and intestines; the site of development showed variation depending on the lineage.
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spelling pubmed-103863832023-07-30 Comparative Analysis of the Exo-Erythrocytic Development of Five Lineages of Haemoproteus majoris, a Common Haemosporidian Parasite of European Passeriform Birds Duc, Mélanie Himmel, Tanja Harl, Josef Iezhova, Tatjana Nedorost, Nora Matt, Julia Ilgūnas, Mikas Weissenböck, Herbert Valkiūnas, Gediminas Pathogens Article Haemoproteus parasites (Apicomplexa, Haemosporida) are widespread pathogens of birds, with a rich genetic (about 1900 lineages) and morphospecies (178 species) diversity. Nonetheless, their life cycles are poorly understood. The exo-erythrocytic stages of three Haemoproteus majoris (widespread generalist parasite) lineages have been previously reported, each in a different bird species. We aimed to further study and compare the development of five H. majoris lineages—hCCF5, hCWT4, hPARUS1, hPHSIB1, and hWW2—in a wider selection of natural avian hosts. A total of 42 individuals belonging to 14 bird species were sampled. Morphospecies and parasitemia were determined by microscopy of blood films, lineages by DNA-barcoding a 478 bp section of the cytochrome b gene, and exo-erythrocytic stages by histology and chromogenic in situ hybridization. The lineage hCWT4 was morphologically characterized as H. majoris for the first time. All lineage infections exclusively featured megalomeronts. The exo-erythrocytic stages found in all examined bird species were similar, particularly for the lineages hCCF5, hPARUS1, and hPHSIB1. Megalomeronts of the lineages hWW2 and hCWT4 were more similar to each other than to the former three lineages. The kidneys and gizzard were most often affected, followed by lungs and intestines; the site of development showed variation depending on the lineage. MDPI 2023-06-30 /pmc/articles/PMC10386383/ /pubmed/37513745 http://dx.doi.org/10.3390/pathogens12070898 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Duc, Mélanie
Himmel, Tanja
Harl, Josef
Iezhova, Tatjana
Nedorost, Nora
Matt, Julia
Ilgūnas, Mikas
Weissenböck, Herbert
Valkiūnas, Gediminas
Comparative Analysis of the Exo-Erythrocytic Development of Five Lineages of Haemoproteus majoris, a Common Haemosporidian Parasite of European Passeriform Birds
title Comparative Analysis of the Exo-Erythrocytic Development of Five Lineages of Haemoproteus majoris, a Common Haemosporidian Parasite of European Passeriform Birds
title_full Comparative Analysis of the Exo-Erythrocytic Development of Five Lineages of Haemoproteus majoris, a Common Haemosporidian Parasite of European Passeriform Birds
title_fullStr Comparative Analysis of the Exo-Erythrocytic Development of Five Lineages of Haemoproteus majoris, a Common Haemosporidian Parasite of European Passeriform Birds
title_full_unstemmed Comparative Analysis of the Exo-Erythrocytic Development of Five Lineages of Haemoproteus majoris, a Common Haemosporidian Parasite of European Passeriform Birds
title_short Comparative Analysis of the Exo-Erythrocytic Development of Five Lineages of Haemoproteus majoris, a Common Haemosporidian Parasite of European Passeriform Birds
title_sort comparative analysis of the exo-erythrocytic development of five lineages of haemoproteus majoris, a common haemosporidian parasite of european passeriform birds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386383/
https://www.ncbi.nlm.nih.gov/pubmed/37513745
http://dx.doi.org/10.3390/pathogens12070898
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