Cargando…

Combining Polymer and Cyclodextrin Strategy for Drug Release of Sulfadiazine from Electrospun Fibers

This study reports the fabrication of polymeric matrices through electrospinning using polymethyl methacrylate (PMMA) and poly(lactic-co-glycolic acid) (PLGA), biocompatible polymers commonly used in medical systems. These polymers were combined with an antibacterial drug, sulfadiazine sodium salt (...

Descripción completa

Detalles Bibliográficos
Autores principales: Morais, Diego C., Fontes, Marina L., Oliveira, Analú B., Gabbai-Armelin, Paulo R., Ferrisse, Túlio M., De Oliveira, Luiz F. C., Brighenti, Fernanda Lourenção, Barud, Hernane S., De Sousa, Frederico B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386385/
https://www.ncbi.nlm.nih.gov/pubmed/37514076
http://dx.doi.org/10.3390/pharmaceutics15071890
_version_ 1785081651582205952
author Morais, Diego C.
Fontes, Marina L.
Oliveira, Analú B.
Gabbai-Armelin, Paulo R.
Ferrisse, Túlio M.
De Oliveira, Luiz F. C.
Brighenti, Fernanda Lourenção
Barud, Hernane S.
De Sousa, Frederico B.
author_facet Morais, Diego C.
Fontes, Marina L.
Oliveira, Analú B.
Gabbai-Armelin, Paulo R.
Ferrisse, Túlio M.
De Oliveira, Luiz F. C.
Brighenti, Fernanda Lourenção
Barud, Hernane S.
De Sousa, Frederico B.
author_sort Morais, Diego C.
collection PubMed
description This study reports the fabrication of polymeric matrices through electrospinning using polymethyl methacrylate (PMMA) and poly(lactic-co-glycolic acid) (PLGA), biocompatible polymers commonly used in medical systems. These polymers were combined with an antibacterial drug, sulfadiazine sodium salt (SDS) or its supramolecular system formed with hydroxypropyl-β-cyclodextrin (HPβ/CD) at 1:1 molar ratio, aiming to assemble a transdermal drug delivery system. The formation of fibers was confirmed by scanning electron microscopy (SEM), and the fibers’ surface properties were analyzed using contact angle and water vapor permeability techniques. Drug release tests and cell viability assays were performed to evaluate the potential toxicity of the material. SEM images demonstrated that the obtained fibers had nanoscale- and micrometer-scale diameters in PLGA and PMMA systems, respectively. The contact angle analyses indicated that, even in the presence of hydrophilic molecules (SDS and HPβCD), PMMA fibers exhibited hydrophobic characteristics, while PLGA fibers exhibited hydrophilic surface properties. These data were also confirmed by water vapor permeability analysis. The drug release profiles demonstrated a greater release of SDS in the PLGA system. Moreover, the presence of HPβCD improved the drug release in both polymeric systems and the cell viability in the PMMA SDS/HPβCD system. In terms of antibacterial activity, all membranes yielded positive outcomes; nevertheless, the PLGA SDS/HPβCD membrane exhibited the most remarkable results, with the lowest microbial load values. Additionally, the pseudo wound healing analysis demonstrated that the PLGA SDS/HPβCD fiber exhibited results similar to the control group. Consequently, these findings exemplify the substantial potential of the obtained materials for use in wound healing applications.
format Online
Article
Text
id pubmed-10386385
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-103863852023-07-30 Combining Polymer and Cyclodextrin Strategy for Drug Release of Sulfadiazine from Electrospun Fibers Morais, Diego C. Fontes, Marina L. Oliveira, Analú B. Gabbai-Armelin, Paulo R. Ferrisse, Túlio M. De Oliveira, Luiz F. C. Brighenti, Fernanda Lourenção Barud, Hernane S. De Sousa, Frederico B. Pharmaceutics Article This study reports the fabrication of polymeric matrices through electrospinning using polymethyl methacrylate (PMMA) and poly(lactic-co-glycolic acid) (PLGA), biocompatible polymers commonly used in medical systems. These polymers were combined with an antibacterial drug, sulfadiazine sodium salt (SDS) or its supramolecular system formed with hydroxypropyl-β-cyclodextrin (HPβ/CD) at 1:1 molar ratio, aiming to assemble a transdermal drug delivery system. The formation of fibers was confirmed by scanning electron microscopy (SEM), and the fibers’ surface properties were analyzed using contact angle and water vapor permeability techniques. Drug release tests and cell viability assays were performed to evaluate the potential toxicity of the material. SEM images demonstrated that the obtained fibers had nanoscale- and micrometer-scale diameters in PLGA and PMMA systems, respectively. The contact angle analyses indicated that, even in the presence of hydrophilic molecules (SDS and HPβCD), PMMA fibers exhibited hydrophobic characteristics, while PLGA fibers exhibited hydrophilic surface properties. These data were also confirmed by water vapor permeability analysis. The drug release profiles demonstrated a greater release of SDS in the PLGA system. Moreover, the presence of HPβCD improved the drug release in both polymeric systems and the cell viability in the PMMA SDS/HPβCD system. In terms of antibacterial activity, all membranes yielded positive outcomes; nevertheless, the PLGA SDS/HPβCD membrane exhibited the most remarkable results, with the lowest microbial load values. Additionally, the pseudo wound healing analysis demonstrated that the PLGA SDS/HPβCD fiber exhibited results similar to the control group. Consequently, these findings exemplify the substantial potential of the obtained materials for use in wound healing applications. MDPI 2023-07-05 /pmc/articles/PMC10386385/ /pubmed/37514076 http://dx.doi.org/10.3390/pharmaceutics15071890 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morais, Diego C.
Fontes, Marina L.
Oliveira, Analú B.
Gabbai-Armelin, Paulo R.
Ferrisse, Túlio M.
De Oliveira, Luiz F. C.
Brighenti, Fernanda Lourenção
Barud, Hernane S.
De Sousa, Frederico B.
Combining Polymer and Cyclodextrin Strategy for Drug Release of Sulfadiazine from Electrospun Fibers
title Combining Polymer and Cyclodextrin Strategy for Drug Release of Sulfadiazine from Electrospun Fibers
title_full Combining Polymer and Cyclodextrin Strategy for Drug Release of Sulfadiazine from Electrospun Fibers
title_fullStr Combining Polymer and Cyclodextrin Strategy for Drug Release of Sulfadiazine from Electrospun Fibers
title_full_unstemmed Combining Polymer and Cyclodextrin Strategy for Drug Release of Sulfadiazine from Electrospun Fibers
title_short Combining Polymer and Cyclodextrin Strategy for Drug Release of Sulfadiazine from Electrospun Fibers
title_sort combining polymer and cyclodextrin strategy for drug release of sulfadiazine from electrospun fibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386385/
https://www.ncbi.nlm.nih.gov/pubmed/37514076
http://dx.doi.org/10.3390/pharmaceutics15071890
work_keys_str_mv AT moraisdiegoc combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers
AT fontesmarinal combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers
AT oliveiraanalub combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers
AT gabbaiarmelinpaulor combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers
AT ferrissetuliom combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers
AT deoliveiraluizfc combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers
AT brighentifernandalourencao combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers
AT barudhernanes combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers
AT desousafredericob combiningpolymerandcyclodextrinstrategyfordrugreleaseofsulfadiazinefromelectrospunfibers