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Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress

With the help of laser ablation, a technology for obtaining nanosized crystalline selenium particles (SeNPs) has been created. The SeNPs do not exhibit significant toxic properties, in contrast to molecular selenium compounds. The administration of SeNPs can significantly increase the viabilities of...

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Autores principales: Gudkov, Sergey V., Gao, Meng, Simakin, Alexander V., Baryshev, Alexey S., Pobedonostsev, Roman V., Baimler, Ilya V., Rebezov, Maksim B., Sarimov, Ruslan M., Astashev, Maxim E., Dikovskaya, Anastasia O., Molkova, Elena A., Kozlov, Valery A., Bunkin, Nikolay F., Sevostyanov, Mikhail A., Kolmakov, Alexey G., Kaplan, Mikhail A., Sharapov, Mars G., Ivanov, Vladimir E., Bruskov, Vadim I., Kalinichenko, Valery P., Aiyyzhy, Kuder O., Voronov, Valery V., Pimpha, Nuttaporn, Li, Ruibin, Shafeev, Georgy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386620/
https://www.ncbi.nlm.nih.gov/pubmed/37512437
http://dx.doi.org/10.3390/ma16145164
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author Gudkov, Sergey V.
Gao, Meng
Simakin, Alexander V.
Baryshev, Alexey S.
Pobedonostsev, Roman V.
Baimler, Ilya V.
Rebezov, Maksim B.
Sarimov, Ruslan M.
Astashev, Maxim E.
Dikovskaya, Anastasia O.
Molkova, Elena A.
Kozlov, Valery A.
Bunkin, Nikolay F.
Sevostyanov, Mikhail A.
Kolmakov, Alexey G.
Kaplan, Mikhail A.
Sharapov, Mars G.
Ivanov, Vladimir E.
Bruskov, Vadim I.
Kalinichenko, Valery P.
Aiyyzhy, Kuder O.
Voronov, Valery V.
Pimpha, Nuttaporn
Li, Ruibin
Shafeev, Georgy A.
author_facet Gudkov, Sergey V.
Gao, Meng
Simakin, Alexander V.
Baryshev, Alexey S.
Pobedonostsev, Roman V.
Baimler, Ilya V.
Rebezov, Maksim B.
Sarimov, Ruslan M.
Astashev, Maxim E.
Dikovskaya, Anastasia O.
Molkova, Elena A.
Kozlov, Valery A.
Bunkin, Nikolay F.
Sevostyanov, Mikhail A.
Kolmakov, Alexey G.
Kaplan, Mikhail A.
Sharapov, Mars G.
Ivanov, Vladimir E.
Bruskov, Vadim I.
Kalinichenko, Valery P.
Aiyyzhy, Kuder O.
Voronov, Valery V.
Pimpha, Nuttaporn
Li, Ruibin
Shafeev, Georgy A.
author_sort Gudkov, Sergey V.
collection PubMed
description With the help of laser ablation, a technology for obtaining nanosized crystalline selenium particles (SeNPs) has been created. The SeNPs do not exhibit significant toxic properties, in contrast to molecular selenium compounds. The administration of SeNPs can significantly increase the viabilities of SH-SY5Y and PCMF cells after radiation exposure. The introduction of such nanoparticles into the animal body protects proteins and DNA from radiation-induced damage. The number of chromosomal breaks and oxidized proteins decreases in irradiated mice treated with SeNPs. Using hematological tests, it was found that a decrease in radiation-induced leukopenia and thrombocytopenia is observed when selenium nanoparticles are injected into mice before exposure to ionizing radiation. The administration of SeNPs to animals 5 h before radiation exposure in sublethal and lethal doses significantly increases their survival rate. The modification dose factor for animal survival was 1.2. It has been shown that the introduction of selenium nanoparticles significantly normalizes gene expression in the cells of the red bone marrow of mice after exposure to ionizing radiation. Thus, it has been demonstrated that SeNPs are a new gene-protective and radioprotective agent that can significantly reduce the harmful effects of ionizing radiation.
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spelling pubmed-103866202023-07-30 Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress Gudkov, Sergey V. Gao, Meng Simakin, Alexander V. Baryshev, Alexey S. Pobedonostsev, Roman V. Baimler, Ilya V. Rebezov, Maksim B. Sarimov, Ruslan M. Astashev, Maxim E. Dikovskaya, Anastasia O. Molkova, Elena A. Kozlov, Valery A. Bunkin, Nikolay F. Sevostyanov, Mikhail A. Kolmakov, Alexey G. Kaplan, Mikhail A. Sharapov, Mars G. Ivanov, Vladimir E. Bruskov, Vadim I. Kalinichenko, Valery P. Aiyyzhy, Kuder O. Voronov, Valery V. Pimpha, Nuttaporn Li, Ruibin Shafeev, Georgy A. Materials (Basel) Article With the help of laser ablation, a technology for obtaining nanosized crystalline selenium particles (SeNPs) has been created. The SeNPs do not exhibit significant toxic properties, in contrast to molecular selenium compounds. The administration of SeNPs can significantly increase the viabilities of SH-SY5Y and PCMF cells after radiation exposure. The introduction of such nanoparticles into the animal body protects proteins and DNA from radiation-induced damage. The number of chromosomal breaks and oxidized proteins decreases in irradiated mice treated with SeNPs. Using hematological tests, it was found that a decrease in radiation-induced leukopenia and thrombocytopenia is observed when selenium nanoparticles are injected into mice before exposure to ionizing radiation. The administration of SeNPs to animals 5 h before radiation exposure in sublethal and lethal doses significantly increases their survival rate. The modification dose factor for animal survival was 1.2. It has been shown that the introduction of selenium nanoparticles significantly normalizes gene expression in the cells of the red bone marrow of mice after exposure to ionizing radiation. Thus, it has been demonstrated that SeNPs are a new gene-protective and radioprotective agent that can significantly reduce the harmful effects of ionizing radiation. MDPI 2023-07-22 /pmc/articles/PMC10386620/ /pubmed/37512437 http://dx.doi.org/10.3390/ma16145164 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gudkov, Sergey V.
Gao, Meng
Simakin, Alexander V.
Baryshev, Alexey S.
Pobedonostsev, Roman V.
Baimler, Ilya V.
Rebezov, Maksim B.
Sarimov, Ruslan M.
Astashev, Maxim E.
Dikovskaya, Anastasia O.
Molkova, Elena A.
Kozlov, Valery A.
Bunkin, Nikolay F.
Sevostyanov, Mikhail A.
Kolmakov, Alexey G.
Kaplan, Mikhail A.
Sharapov, Mars G.
Ivanov, Vladimir E.
Bruskov, Vadim I.
Kalinichenko, Valery P.
Aiyyzhy, Kuder O.
Voronov, Valery V.
Pimpha, Nuttaporn
Li, Ruibin
Shafeev, Georgy A.
Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress
title Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress
title_full Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress
title_fullStr Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress
title_full_unstemmed Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress
title_short Laser Ablation-Generated Crystalline Selenium Nanoparticles Prevent Damage of DNA and Proteins Induced by Reactive Oxygen Species and Protect Mice against Injuries Caused by Radiation-Induced Oxidative Stress
title_sort laser ablation-generated crystalline selenium nanoparticles prevent damage of dna and proteins induced by reactive oxygen species and protect mice against injuries caused by radiation-induced oxidative stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386620/
https://www.ncbi.nlm.nih.gov/pubmed/37512437
http://dx.doi.org/10.3390/ma16145164
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