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Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster
Temperature influences male fertility across organisms; however, how suboptimal temperatures affect adult spermatogenesis remains understudied. In a recent study on Drosophila melanogaster oogenesis, we observed a drastic reduction in the fertility of adult males exposed to warm temperature (29 °C)....
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387475/ https://www.ncbi.nlm.nih.gov/pubmed/37518578 http://dx.doi.org/10.1038/s41598-023-39360-7 |
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author | Gandara, Ana Caroline P. Drummond-Barbosa, Daniela |
author_facet | Gandara, Ana Caroline P. Drummond-Barbosa, Daniela |
author_sort | Gandara, Ana Caroline P. |
collection | PubMed |
description | Temperature influences male fertility across organisms; however, how suboptimal temperatures affect adult spermatogenesis remains understudied. In a recent study on Drosophila melanogaster oogenesis, we observed a drastic reduction in the fertility of adult males exposed to warm temperature (29 °C). Here, we show that males become infertile at 29 °C because of low sperm abundance and quality. The low sperm abundance at 29 °C does not stem from reduced germline stem cell or spermatid numbers, as those numbers remain comparable between 29 °C and control 25 °C. Notably, males at cold 18 °C and 29 °C had similarly increased frequencies of spermatid elongation and individualization defects which, considering the high sperm abundance and male fertility measured at 18 °C, indicate that spermatogenesis has a high tolerance for elongation and individualization defects. Interestingly, the abundance of sperm at 29 °C decreases abruptly and with no evidence of apoptosis as they transition into the seminal vesicle near the end of spermatogenesis, pointing to sperm elimination through an unknown mechanism. Finally, sperm from males at 29 °C fertilize eggs less efficiently and do not support embryos past the first stage of embryogenesis, indicating that poor sperm quality is an additional cause of male infertility at 29 °C. |
format | Online Article Text |
id | pubmed-10387475 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103874752023-08-01 Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster Gandara, Ana Caroline P. Drummond-Barbosa, Daniela Sci Rep Article Temperature influences male fertility across organisms; however, how suboptimal temperatures affect adult spermatogenesis remains understudied. In a recent study on Drosophila melanogaster oogenesis, we observed a drastic reduction in the fertility of adult males exposed to warm temperature (29 °C). Here, we show that males become infertile at 29 °C because of low sperm abundance and quality. The low sperm abundance at 29 °C does not stem from reduced germline stem cell or spermatid numbers, as those numbers remain comparable between 29 °C and control 25 °C. Notably, males at cold 18 °C and 29 °C had similarly increased frequencies of spermatid elongation and individualization defects which, considering the high sperm abundance and male fertility measured at 18 °C, indicate that spermatogenesis has a high tolerance for elongation and individualization defects. Interestingly, the abundance of sperm at 29 °C decreases abruptly and with no evidence of apoptosis as they transition into the seminal vesicle near the end of spermatogenesis, pointing to sperm elimination through an unknown mechanism. Finally, sperm from males at 29 °C fertilize eggs less efficiently and do not support embryos past the first stage of embryogenesis, indicating that poor sperm quality is an additional cause of male infertility at 29 °C. Nature Publishing Group UK 2023-07-30 /pmc/articles/PMC10387475/ /pubmed/37518578 http://dx.doi.org/10.1038/s41598-023-39360-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Gandara, Ana Caroline P. Drummond-Barbosa, Daniela Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster |
title | Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster |
title_full | Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster |
title_fullStr | Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster |
title_full_unstemmed | Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster |
title_short | Chronic exposure to warm temperature causes low sperm abundance and quality in Drosophila melanogaster |
title_sort | chronic exposure to warm temperature causes low sperm abundance and quality in drosophila melanogaster |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387475/ https://www.ncbi.nlm.nih.gov/pubmed/37518578 http://dx.doi.org/10.1038/s41598-023-39360-7 |
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