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Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6
INTRODUCTION: Extremely salt-tolerant microorganisms play an important role in the development of functional metabolites or drug molecules. METHODS: In this work, the salt stress perception and metabolic regulation network of a marine probiotic Meyerozyma guilliermondii GXDK6 were investigated using...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387536/ https://www.ncbi.nlm.nih.gov/pubmed/37529325 http://dx.doi.org/10.3389/fmicb.2023.1193352 |
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author | Cai, Xinghua Sun, Huijie Yan, Bing Bai, Huashan Zhou, Xing Shen, Peihong Jiang, Chengjian |
author_facet | Cai, Xinghua Sun, Huijie Yan, Bing Bai, Huashan Zhou, Xing Shen, Peihong Jiang, Chengjian |
author_sort | Cai, Xinghua |
collection | PubMed |
description | INTRODUCTION: Extremely salt-tolerant microorganisms play an important role in the development of functional metabolites or drug molecules. METHODS: In this work, the salt stress perception and metabolic regulation network of a marine probiotic Meyerozyma guilliermondii GXDK6 were investigated using integrative omics technology. RESULTS: Results indicated that GXDK6 could accept the salt stress signals from signal transduction proteins (e.g., phosphorelay intermediate protein YPD1), thereby contributing to regulating the differential expression of its relevant genes (e.g., CTT1, SOD) and proteins (e.g., catalase, superoxide dismutase) in response to salt stress, and increasing the salt-tolerant viability of GXDK6. Omics data also suggested that the transcription (e.g., SMD2), translation (e.g., MRPL1), and protein synthesis and processing (e.g., inner membrane protein OXA1) of upregulated RNAs may contribute to increasing the salt-tolerant survivability of GXDK6 by improving protein transport activity (e.g., Small nuclear ribonucleoprotein Sm D2), anti-apoptotic ability (e.g., 54S ribosomal protein L1), and antioxidant activity (e.g., superoxide dismutase). Moreover, up to 65.9% of the differentially expressed genes/proteins could stimulate GXDK6 to biosynthesize many salt tolerant-related metabolites (e.g., β-alanine, D-mannose) and drug molecules (e.g., deoxyspergualin, calcitriol), and were involved in the metabolic regulation of GXDK6 under high NaCl stress. DISCUSSION: This study provided new insights into the exploration of novel functional products and/or drugs from extremely salt-tolerant microorganisms. |
format | Online Article Text |
id | pubmed-10387536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103875362023-08-01 Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6 Cai, Xinghua Sun, Huijie Yan, Bing Bai, Huashan Zhou, Xing Shen, Peihong Jiang, Chengjian Front Microbiol Microbiology INTRODUCTION: Extremely salt-tolerant microorganisms play an important role in the development of functional metabolites or drug molecules. METHODS: In this work, the salt stress perception and metabolic regulation network of a marine probiotic Meyerozyma guilliermondii GXDK6 were investigated using integrative omics technology. RESULTS: Results indicated that GXDK6 could accept the salt stress signals from signal transduction proteins (e.g., phosphorelay intermediate protein YPD1), thereby contributing to regulating the differential expression of its relevant genes (e.g., CTT1, SOD) and proteins (e.g., catalase, superoxide dismutase) in response to salt stress, and increasing the salt-tolerant viability of GXDK6. Omics data also suggested that the transcription (e.g., SMD2), translation (e.g., MRPL1), and protein synthesis and processing (e.g., inner membrane protein OXA1) of upregulated RNAs may contribute to increasing the salt-tolerant survivability of GXDK6 by improving protein transport activity (e.g., Small nuclear ribonucleoprotein Sm D2), anti-apoptotic ability (e.g., 54S ribosomal protein L1), and antioxidant activity (e.g., superoxide dismutase). Moreover, up to 65.9% of the differentially expressed genes/proteins could stimulate GXDK6 to biosynthesize many salt tolerant-related metabolites (e.g., β-alanine, D-mannose) and drug molecules (e.g., deoxyspergualin, calcitriol), and were involved in the metabolic regulation of GXDK6 under high NaCl stress. DISCUSSION: This study provided new insights into the exploration of novel functional products and/or drugs from extremely salt-tolerant microorganisms. Frontiers Media S.A. 2023-07-17 /pmc/articles/PMC10387536/ /pubmed/37529325 http://dx.doi.org/10.3389/fmicb.2023.1193352 Text en Copyright © 2023 Cai, Sun, Yan, Bai, Zhou, Shen and Jiang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Cai, Xinghua Sun, Huijie Yan, Bing Bai, Huashan Zhou, Xing Shen, Peihong Jiang, Chengjian Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6 |
title | Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6 |
title_full | Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6 |
title_fullStr | Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6 |
title_full_unstemmed | Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6 |
title_short | Salt stress perception and metabolic regulation network analysis of a marine probiotic Meyerozyma guilliermondii GXDK6 |
title_sort | salt stress perception and metabolic regulation network analysis of a marine probiotic meyerozyma guilliermondii gxdk6 |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387536/ https://www.ncbi.nlm.nih.gov/pubmed/37529325 http://dx.doi.org/10.3389/fmicb.2023.1193352 |
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