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Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice

BACKGROUND: Early dietary introduction of peanut has shown efficacy in clinical trials and driven pediatric recommendations for early introduction of peanut to children with heightened allergy risk worldwide. Unfortunately, tolerance is not induced in every case, and a subset of patients are allergi...

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Autores principales: Immormino, Robert M., Smeekens, Johanna M., Mathai, Priscilla I., Kesselring, Janelle R., Turner, Andrew V., Kulis, Michael D., Moran, Timothy P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387557/
https://www.ncbi.nlm.nih.gov/pubmed/37528863
http://dx.doi.org/10.3389/falgy.2023.1219268
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author Immormino, Robert M.
Smeekens, Johanna M.
Mathai, Priscilla I.
Kesselring, Janelle R.
Turner, Andrew V.
Kulis, Michael D.
Moran, Timothy P.
author_facet Immormino, Robert M.
Smeekens, Johanna M.
Mathai, Priscilla I.
Kesselring, Janelle R.
Turner, Andrew V.
Kulis, Michael D.
Moran, Timothy P.
author_sort Immormino, Robert M.
collection PubMed
description BACKGROUND: Early dietary introduction of peanut has shown efficacy in clinical trials and driven pediatric recommendations for early introduction of peanut to children with heightened allergy risk worldwide. Unfortunately, tolerance is not induced in every case, and a subset of patients are allergic prior to introduction. Here we assess peanut allergic sensitization and oral tolerance in genetically diverse mouse strains. OBJECTIVE: We aimed to determine whether environmental adjuvant-driven airway sensitization and oral tolerance to peanut could be induced in various genetically diverse mouse strains. METHODS: C57BL/6J and 12 Collaborative Cross (CC) mouse strains were fed regular chow or ad libitum peanut butter to induce tolerance. Tolerance was tested by attempting to sensitize mice via intratracheal exposure to peanut and lipopolysaccharide (LPS), followed by intraperitoneal peanut challenge. Peanut-specific immunoglobulins and peanut-induced anaphylaxis were assessed. RESULTS: Without oral peanut feeding, most CC strains (11/12) and C57BL/6J induced peanut-specific IgE and IgG1 following airway exposure to peanut and LPS. With oral peanut feeding none of the CC strains nor C57BL/6J mice became sensitized to peanut or experienced anaphylaxis following peanut challenge. CONCLUSION: Allergic sensitization and oral tolerance to peanut can be achieved across a range of genetically diverse mice. Notably, the same strains that became allergic via airway sensitization were tolerized by feeding high doses of peanut butter before sensitization, suggesting that the order and route of peanut exposure are critical for determining the allergic fate.
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spelling pubmed-103875572023-08-01 Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice Immormino, Robert M. Smeekens, Johanna M. Mathai, Priscilla I. Kesselring, Janelle R. Turner, Andrew V. Kulis, Michael D. Moran, Timothy P. Front Allergy Allergy BACKGROUND: Early dietary introduction of peanut has shown efficacy in clinical trials and driven pediatric recommendations for early introduction of peanut to children with heightened allergy risk worldwide. Unfortunately, tolerance is not induced in every case, and a subset of patients are allergic prior to introduction. Here we assess peanut allergic sensitization and oral tolerance in genetically diverse mouse strains. OBJECTIVE: We aimed to determine whether environmental adjuvant-driven airway sensitization and oral tolerance to peanut could be induced in various genetically diverse mouse strains. METHODS: C57BL/6J and 12 Collaborative Cross (CC) mouse strains were fed regular chow or ad libitum peanut butter to induce tolerance. Tolerance was tested by attempting to sensitize mice via intratracheal exposure to peanut and lipopolysaccharide (LPS), followed by intraperitoneal peanut challenge. Peanut-specific immunoglobulins and peanut-induced anaphylaxis were assessed. RESULTS: Without oral peanut feeding, most CC strains (11/12) and C57BL/6J induced peanut-specific IgE and IgG1 following airway exposure to peanut and LPS. With oral peanut feeding none of the CC strains nor C57BL/6J mice became sensitized to peanut or experienced anaphylaxis following peanut challenge. CONCLUSION: Allergic sensitization and oral tolerance to peanut can be achieved across a range of genetically diverse mice. Notably, the same strains that became allergic via airway sensitization were tolerized by feeding high doses of peanut butter before sensitization, suggesting that the order and route of peanut exposure are critical for determining the allergic fate. Frontiers Media S.A. 2023-07-17 /pmc/articles/PMC10387557/ /pubmed/37528863 http://dx.doi.org/10.3389/falgy.2023.1219268 Text en © 2023 Immormino, Smeekens, Mathai, Kesselring, Turner, Kulis and Moran. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Allergy
Immormino, Robert M.
Smeekens, Johanna M.
Mathai, Priscilla I.
Kesselring, Janelle R.
Turner, Andrew V.
Kulis, Michael D.
Moran, Timothy P.
Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice
title Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice
title_full Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice
title_fullStr Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice
title_full_unstemmed Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice
title_short Peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice
title_sort peanut butter feeding induces oral tolerance in genetically diverse collaborative cross mice
topic Allergy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387557/
https://www.ncbi.nlm.nih.gov/pubmed/37528863
http://dx.doi.org/10.3389/falgy.2023.1219268
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