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Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells

BACKGROUND/AIM: The study aims to profile the dual-specificity phosphatases (DUSP) expression in response to Transforming growth factor β1 (TGFβ1)-induced epithelial-mesenchymal transition (EMT) in ovarian adenocarcinoma cells. MATERIALS AND METHODS: The ovarian adenocarcinoma cell line SKOV3 was us...

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Autores principales: GÜLER, Sabire, YALÇIN, Abdullah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Scientific and Technological Research Council of Turkey (TUBITAK) 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387886/
https://www.ncbi.nlm.nih.gov/pubmed/37476896
http://dx.doi.org/10.55730/1300-0144.5626
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author GÜLER, Sabire
YALÇIN, Abdullah
author_facet GÜLER, Sabire
YALÇIN, Abdullah
author_sort GÜLER, Sabire
collection PubMed
description BACKGROUND/AIM: The study aims to profile the dual-specificity phosphatases (DUSP) expression in response to Transforming growth factor β1 (TGFβ1)-induced epithelial-mesenchymal transition (EMT) in ovarian adenocarcinoma cells. MATERIALS AND METHODS: The ovarian adenocarcinoma cell line SKOV3 was used as a TGFβ1-induced EMT model. Cells were incubated with 5 ng/mL TGFβ1 to induce EMT. EMT was confirmed with real-time qPCR, western blot, and immunofluorescence analyses of various EMT markers. Western blot was used to analyze phospho- and total MAPK protein levels. Typical and atypical DUSPs mRNA expression profile was determined by real-time qPCR. RESULTS: The epithelial marker E-cadherin expressions were decreased and mesenchymal EMT markers Snail and Slug expression levels were increased after TGFβ1 induction. Phosphorylation of ERK1/2 and p38 MAPK were enhanced in response to TGFβ1 treatment. The expression of DUSP2, DUSP6, DUSP8, DUSP10, and DUSP13 were decreased while DUSP7, DUSP16, DUSP18, DUSP21, and DUSP27 were increased by TGFβ1. CONCLUSION: TGFβ1 induced EMT which was accompanied by increased activity of MAPKs, and led to marked changes in expressions of several DUSPs in SKOV3 cells.
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spelling pubmed-103878862023-08-01 Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells GÜLER, Sabire YALÇIN, Abdullah Turk J Med Sci Research Article BACKGROUND/AIM: The study aims to profile the dual-specificity phosphatases (DUSP) expression in response to Transforming growth factor β1 (TGFβ1)-induced epithelial-mesenchymal transition (EMT) in ovarian adenocarcinoma cells. MATERIALS AND METHODS: The ovarian adenocarcinoma cell line SKOV3 was used as a TGFβ1-induced EMT model. Cells were incubated with 5 ng/mL TGFβ1 to induce EMT. EMT was confirmed with real-time qPCR, western blot, and immunofluorescence analyses of various EMT markers. Western blot was used to analyze phospho- and total MAPK protein levels. Typical and atypical DUSPs mRNA expression profile was determined by real-time qPCR. RESULTS: The epithelial marker E-cadherin expressions were decreased and mesenchymal EMT markers Snail and Slug expression levels were increased after TGFβ1 induction. Phosphorylation of ERK1/2 and p38 MAPK were enhanced in response to TGFβ1 treatment. The expression of DUSP2, DUSP6, DUSP8, DUSP10, and DUSP13 were decreased while DUSP7, DUSP16, DUSP18, DUSP21, and DUSP27 were increased by TGFβ1. CONCLUSION: TGFβ1 induced EMT which was accompanied by increased activity of MAPKs, and led to marked changes in expressions of several DUSPs in SKOV3 cells. Scientific and Technological Research Council of Turkey (TUBITAK) 2023-02-21 /pmc/articles/PMC10387886/ /pubmed/37476896 http://dx.doi.org/10.55730/1300-0144.5626 Text en © TÜBİTAK https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
GÜLER, Sabire
YALÇIN, Abdullah
Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells
title Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells
title_full Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells
title_fullStr Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells
title_full_unstemmed Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells
title_short Expression of dual-specificity phosphatases in TGFβ1-induced EMT in SKOV3 cells
title_sort expression of dual-specificity phosphatases in tgfβ1-induced emt in skov3 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10387886/
https://www.ncbi.nlm.nih.gov/pubmed/37476896
http://dx.doi.org/10.55730/1300-0144.5626
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