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Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models

Since obesity causes at least 2.8 million death each year and is a major risk factor for many diseases, it is critical to evaluate alternative treatment approaches. In this context, studies on the research of natural product-based therapeutics in the fight against obesity are increasing. In this stu...

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Autores principales: GÜLNAR, Birgül, CANATAR, İpek, ÖZDAŞ, Sibel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Scientific and Technological Research Council of Turkey (TUBITAK) 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388082/
https://www.ncbi.nlm.nih.gov/pubmed/37529167
http://dx.doi.org/10.55730/1300-0152.2649
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author GÜLNAR, Birgül
CANATAR, İpek
ÖZDAŞ, Sibel
author_facet GÜLNAR, Birgül
CANATAR, İpek
ÖZDAŞ, Sibel
author_sort GÜLNAR, Birgül
collection PubMed
description Since obesity causes at least 2.8 million death each year and is a major risk factor for many diseases, it is critical to evaluate alternative treatment approaches. In this context, studies on the research of natural product-based therapeutics in the fight against obesity are increasing. In this study, it was aimed to evaluate the antiadipogenic and antiobesogenic efficacy of pterostilbene a natural phenolic compound in 3T3-L1 cells. The mature 3T3-L1 adipocytes were exposed to pterostilbene at different concentrations and half-maximum inhibitory concentrations (IC(50)) were determined by MTT assay. Oil-Red-O staining was applied to determine lipid accumulation. Phase contrast microscopy, Giemsa, F-actin and DAPI staining were applied to examine the efficacy of pterostilbene on the morphology of 3T3-L1 adipocyte cells. Moreover, expressions of adinopectin and glucose transporter-4 (Glut-4) in relation to insulin resistance were evaluated using immunofluorescent staining and qRT-PCR. Pterostilbene caused no significant cytotoxicity towards preadipocytes at concentrations ≤7.5 −M and the percentage of viable cells remained above about 86% for 24 h, 48 h and 72 h (p > 0.05). Therefore, pterostilbene treatment at 5 and 7.5 −M was used in the subsequent experiments as safe dosages. In addition, it was observed that pterostilbene treatment reduced lipid accumulation in adipocyte differentiation. Adipocytes treated with a dose of 7.5 −M for 14 days showed less intense lipid deposition and a more spindle-like morphology compared to the adipocytes treated with a dose of 5 −M. Especially on the 14th day, actin filaments were filamentous in adipocytes treated with pterostilbene 7.5 −M compared to the adipocytes treated with a dose of 5 −M; the filaments were similarly oriented as in preadipocytes, and chromatin condensation was observed to be quite high. Our data suggests that the pterostilbene supplementation may help weight control and the antiadipogenic and that antiobesogenic activity is mediated in part by reduction of lipid accumulation and induction of Glut-4 and Adiponectin levels.
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spelling pubmed-103880822023-08-01 Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models GÜLNAR, Birgül CANATAR, İpek ÖZDAŞ, Sibel Turk J Biol Research Article Since obesity causes at least 2.8 million death each year and is a major risk factor for many diseases, it is critical to evaluate alternative treatment approaches. In this context, studies on the research of natural product-based therapeutics in the fight against obesity are increasing. In this study, it was aimed to evaluate the antiadipogenic and antiobesogenic efficacy of pterostilbene a natural phenolic compound in 3T3-L1 cells. The mature 3T3-L1 adipocytes were exposed to pterostilbene at different concentrations and half-maximum inhibitory concentrations (IC(50)) were determined by MTT assay. Oil-Red-O staining was applied to determine lipid accumulation. Phase contrast microscopy, Giemsa, F-actin and DAPI staining were applied to examine the efficacy of pterostilbene on the morphology of 3T3-L1 adipocyte cells. Moreover, expressions of adinopectin and glucose transporter-4 (Glut-4) in relation to insulin resistance were evaluated using immunofluorescent staining and qRT-PCR. Pterostilbene caused no significant cytotoxicity towards preadipocytes at concentrations ≤7.5 −M and the percentage of viable cells remained above about 86% for 24 h, 48 h and 72 h (p > 0.05). Therefore, pterostilbene treatment at 5 and 7.5 −M was used in the subsequent experiments as safe dosages. In addition, it was observed that pterostilbene treatment reduced lipid accumulation in adipocyte differentiation. Adipocytes treated with a dose of 7.5 −M for 14 days showed less intense lipid deposition and a more spindle-like morphology compared to the adipocytes treated with a dose of 5 −M. Especially on the 14th day, actin filaments were filamentous in adipocytes treated with pterostilbene 7.5 −M compared to the adipocytes treated with a dose of 5 −M; the filaments were similarly oriented as in preadipocytes, and chromatin condensation was observed to be quite high. Our data suggests that the pterostilbene supplementation may help weight control and the antiadipogenic and that antiobesogenic activity is mediated in part by reduction of lipid accumulation and induction of Glut-4 and Adiponectin levels. Scientific and Technological Research Council of Turkey (TUBITAK) 2023-02-21 /pmc/articles/PMC10388082/ /pubmed/37529167 http://dx.doi.org/10.55730/1300-0152.2649 Text en © TÜBİTAK https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
GÜLNAR, Birgül
CANATAR, İpek
ÖZDAŞ, Sibel
Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models
title Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models
title_full Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models
title_fullStr Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models
title_full_unstemmed Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models
title_short Antiadipogenic and antiobesogenic effects of pterostilbene in 3T3-L1 preadipocyte models
title_sort antiadipogenic and antiobesogenic effects of pterostilbene in 3t3-l1 preadipocyte models
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388082/
https://www.ncbi.nlm.nih.gov/pubmed/37529167
http://dx.doi.org/10.55730/1300-0152.2649
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