Cancer testis antigen PASD1 expression and immunogenicity in human colorectal cancer and polyps

Colorectal cancer (CRC) is a malignant tumor arising from a human inner colon lining that may spread to other organs such as the liver and lungs. Per ARNT Sim domain containing 1 (PASD1) is a cancer-testis antigen expressed in cancers including CRC but not in normal tissues except for normal testes....

Descripción completa

Detalles Bibliográficos
Autores principales: SUKOR, Nur Fazilah, YAHAYA, Azyani, SAGAP, Ismail, JAMAL, Rahman, JOHDI, Nor Adzimah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Scientific and Technological Research Council of Turkey (TUBITAK) 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388097/
https://www.ncbi.nlm.nih.gov/pubmed/37529004
http://dx.doi.org/10.55730/1300-0152.2623
_version_ 1785082035207929856
author SUKOR, Nur Fazilah
YAHAYA, Azyani
SAGAP, Ismail
JAMAL, Rahman
JOHDI, Nor Adzimah
author_facet SUKOR, Nur Fazilah
YAHAYA, Azyani
SAGAP, Ismail
JAMAL, Rahman
JOHDI, Nor Adzimah
author_sort SUKOR, Nur Fazilah
collection PubMed
description Colorectal cancer (CRC) is a malignant tumor arising from a human inner colon lining that may spread to other organs such as the liver and lungs. Per ARNT Sim domain containing 1 (PASD1) is a cancer-testis antigen expressed in cancers including CRC but not in normal tissues except for normal testes. This study aims to study PASD1 protein as a potential target for CRC immunotherapy. A total of 90 CRC and polyps tissue samples were investigated for PASD1 RNA and protein expression using a real-time polymerase chain reaction and immunohistochemical staining, respectively. Matched patients’ peripheral blood mononuclear cells were pulsed with PASD1 peptides and measured for immunogenicity, cell cytotoxicity, and cytokine assays. The clinical data were collected and analyzed accordingly. Our results show that PASD1_v2 mRNA expression was highly expressed in CRC (46.0%) and polyps samples (33.3%). Both PASD1-1 and PASD1-2 proteins were expressed in 31.7% of CRC and 29.4% of polyps samples. Protein expression was weak to moderate positive in the cytoplasm and/or nucleus of the tissues. Immune responses towards CD4-specific PASD1 peptides were detected in 21.7% of CRC and 23.5% of polyps patients. The most immunogenic peptide was PASD1 (1) in CRC while PASD1 (3) in polyps. Cytotoxicity effects were detected up to 57.20% observed in CRC samples while IL-17A and IL-6 cytokines were highly expressed. The demographic data suggest that Chinese female patients more than 60 years old, diagnosed with late-stage rectosigmoid tumors may benefit from the PASD1 peptide immunotherapy approach. This is the first report describing CD4-positive T-helper response to the PASD1 positive CRC patients and its cytotoxicity.
format Online
Article
Text
id pubmed-10388097
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Scientific and Technological Research Council of Turkey (TUBITAK)
record_format MEDLINE/PubMed
spelling pubmed-103880972023-08-01 Cancer testis antigen PASD1 expression and immunogenicity in human colorectal cancer and polyps SUKOR, Nur Fazilah YAHAYA, Azyani SAGAP, Ismail JAMAL, Rahman JOHDI, Nor Adzimah Turk J Biol Research Article Colorectal cancer (CRC) is a malignant tumor arising from a human inner colon lining that may spread to other organs such as the liver and lungs. Per ARNT Sim domain containing 1 (PASD1) is a cancer-testis antigen expressed in cancers including CRC but not in normal tissues except for normal testes. This study aims to study PASD1 protein as a potential target for CRC immunotherapy. A total of 90 CRC and polyps tissue samples were investigated for PASD1 RNA and protein expression using a real-time polymerase chain reaction and immunohistochemical staining, respectively. Matched patients’ peripheral blood mononuclear cells were pulsed with PASD1 peptides and measured for immunogenicity, cell cytotoxicity, and cytokine assays. The clinical data were collected and analyzed accordingly. Our results show that PASD1_v2 mRNA expression was highly expressed in CRC (46.0%) and polyps samples (33.3%). Both PASD1-1 and PASD1-2 proteins were expressed in 31.7% of CRC and 29.4% of polyps samples. Protein expression was weak to moderate positive in the cytoplasm and/or nucleus of the tissues. Immune responses towards CD4-specific PASD1 peptides were detected in 21.7% of CRC and 23.5% of polyps patients. The most immunogenic peptide was PASD1 (1) in CRC while PASD1 (3) in polyps. Cytotoxicity effects were detected up to 57.20% observed in CRC samples while IL-17A and IL-6 cytokines were highly expressed. The demographic data suggest that Chinese female patients more than 60 years old, diagnosed with late-stage rectosigmoid tumors may benefit from the PASD1 peptide immunotherapy approach. This is the first report describing CD4-positive T-helper response to the PASD1 positive CRC patients and its cytotoxicity. Scientific and Technological Research Council of Turkey (TUBITAK) 2022-07-14 /pmc/articles/PMC10388097/ /pubmed/37529004 http://dx.doi.org/10.55730/1300-0152.2623 Text en © TÜBİTAK https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License.
spellingShingle Research Article
SUKOR, Nur Fazilah
YAHAYA, Azyani
SAGAP, Ismail
JAMAL, Rahman
JOHDI, Nor Adzimah
Cancer testis antigen PASD1 expression and immunogenicity in human colorectal cancer and polyps
title Cancer testis antigen PASD1 expression and immunogenicity in human colorectal cancer and polyps
title_full Cancer testis antigen PASD1 expression and immunogenicity in human colorectal cancer and polyps
title_fullStr Cancer testis antigen PASD1 expression and immunogenicity in human colorectal cancer and polyps
title_full_unstemmed Cancer testis antigen PASD1 expression and immunogenicity in human colorectal cancer and polyps
title_short Cancer testis antigen PASD1 expression and immunogenicity in human colorectal cancer and polyps
title_sort cancer testis antigen pasd1 expression and immunogenicity in human colorectal cancer and polyps
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388097/
https://www.ncbi.nlm.nih.gov/pubmed/37529004
http://dx.doi.org/10.55730/1300-0152.2623
work_keys_str_mv AT sukornurfazilah cancertestisantigenpasd1expressionandimmunogenicityinhumancolorectalcancerandpolyps
AT yahayaazyani cancertestisantigenpasd1expressionandimmunogenicityinhumancolorectalcancerandpolyps
AT sagapismail cancertestisantigenpasd1expressionandimmunogenicityinhumancolorectalcancerandpolyps
AT jamalrahman cancertestisantigenpasd1expressionandimmunogenicityinhumancolorectalcancerandpolyps
AT johdinoradzimah cancertestisantigenpasd1expressionandimmunogenicityinhumancolorectalcancerandpolyps