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The role of vitamin D-synthesizing enzyme CYP27B1 in systemic lupus erythematosus

BACKGROUND/AIM: To measure the expression of 1α-hydroxylase (CYP27B1) and serum 25(OH)D concentration in systemic lupus erythematosus (SLE) and to investigate the role of CYP27B1 in SLE. MATERIALS AND METHODS: Seventy-seven SLE patients and 35 healthy controls (HCs) were enrolled from September 2017...

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Detalles Bibliográficos
Autores principales: LUO, Man, LIU, Jing, YUAN, Yeshuang, CHEN, Yong, YUAN, Guohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Scientific and Technological Research Council of Turkey (TUBITAK) 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388113/
https://www.ncbi.nlm.nih.gov/pubmed/36326421
http://dx.doi.org/10.55730/1300-0144.5399
Descripción
Sumario:BACKGROUND/AIM: To measure the expression of 1α-hydroxylase (CYP27B1) and serum 25(OH)D concentration in systemic lupus erythematosus (SLE) and to investigate the role of CYP27B1 in SLE. MATERIALS AND METHODS: Seventy-seven SLE patients and 35 healthy controls (HCs) were enrolled from September 2017 to January 2020. The study design is cross-sectional. mRNA expression of CYP27B1 in peripheral blood mononuclear cells (PBMCs) was measured by reverse-transcription quantitative PCR, the protein level of CYP27B1 was quantified by western blotting, and the serum level of 25(OH)D was determined by an enzyme-linked immunosorbent assay. RESULTS: The mRNA expression of CYP27B1 in PBMCs was significantly lower in SLE patients than in HCs (p < 0.001), and the protein quantification confirmed that CYP27B1 expression was lower in SLE patients than in HCs (p = 0.001). Among SLE patients, the prevalence of lupus nephritis was higher in a subgroup with lower CYP27B1 mRNA expression than in a subgroup with normal CYP27B1 mRNA expression (41.07% vs. 14.28%, p = 0.028). The mRNA expression of CYP27B1 negatively correlated with the Systemic Lupus Erythematosus Disease Activity Index (r = −0.331, p = 0.003). Serum 25(OH)D concentration was lower in SLE patients than in HCs (37.64 ± 19.89 vs. 50.58 ± 12.74 ng/mL, mean ± SD, p = 0.003). CONCLUSION: The expression of CYP27B1 in PBMCs may be related to SLE pathogenesis, disease activity, and nephritis.