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Detection of bacteria causing mastitis in cows with immunoelectric tool
BACKGROUND: This study has investigated designing, producing, and evaluating a newly developed immunoelectric device for the identification of the Staphylococcus aureus and Escherichia coli in experimentally or clinically polluted milk samples. METHODS: The design of the immunoelectric tool parts wa...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388230/ https://www.ncbi.nlm.nih.gov/pubmed/37352319 http://dx.doi.org/10.1002/jcla.24939 |
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author | Mohammad, Khosravi Meysam, Makki MohammadNasir, Ghoudarzi Darioush, Gharibi |
author_facet | Mohammad, Khosravi Meysam, Makki MohammadNasir, Ghoudarzi Darioush, Gharibi |
author_sort | Mohammad, Khosravi |
collection | PubMed |
description | BACKGROUND: This study has investigated designing, producing, and evaluating a newly developed immunoelectric device for the identification of the Staphylococcus aureus and Escherichia coli in experimentally or clinically polluted milk samples. METHODS: The design of the immunoelectric tool parts was carried out and produced by using a 3D printer. The animals were immunized against S. aureus and E. coli; purification of the specific IgG from hyperimmune serum was carried out by ion exchange and affinity chromatography methods. Coupling of the specific rabbit antibodies to the polystyrene filters was performed by DTPA linker. The purified rat antibodies were coupled to Fe nanoparticles and used as detector elements. The experimentally polluted milk and PBS samples were prepared. After optimization, the minimum traceable number of the bacteria was determined using immunoelectric tool. Also, the cow's milk samples with clinical mastitis were tested for bacterial detection by bacterial culture and immunoelectric methods. RESULTS: Coupling of the antibody to the filters and capturing of the target bacteria to the filters were successfully confirmed using enzyme immune assay and electron microscopy. The detection limit of the developed immunoelectric tool was equal to 15 cells/mL in PBS or milk samples for 30 min. Also, in agreement with bacterial cell culture, the clinically infected milk samples by S. aureus and E. coli were successfully detected using the immunoelectric device. CONCLUSIONS: The developed immunoelectric device could be utilized for rapid and cost–benefit detection of the bacterial organism. |
format | Online Article Text |
id | pubmed-10388230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103882302023-08-01 Detection of bacteria causing mastitis in cows with immunoelectric tool Mohammad, Khosravi Meysam, Makki MohammadNasir, Ghoudarzi Darioush, Gharibi J Clin Lab Anal Research Articles BACKGROUND: This study has investigated designing, producing, and evaluating a newly developed immunoelectric device for the identification of the Staphylococcus aureus and Escherichia coli in experimentally or clinically polluted milk samples. METHODS: The design of the immunoelectric tool parts was carried out and produced by using a 3D printer. The animals were immunized against S. aureus and E. coli; purification of the specific IgG from hyperimmune serum was carried out by ion exchange and affinity chromatography methods. Coupling of the specific rabbit antibodies to the polystyrene filters was performed by DTPA linker. The purified rat antibodies were coupled to Fe nanoparticles and used as detector elements. The experimentally polluted milk and PBS samples were prepared. After optimization, the minimum traceable number of the bacteria was determined using immunoelectric tool. Also, the cow's milk samples with clinical mastitis were tested for bacterial detection by bacterial culture and immunoelectric methods. RESULTS: Coupling of the antibody to the filters and capturing of the target bacteria to the filters were successfully confirmed using enzyme immune assay and electron microscopy. The detection limit of the developed immunoelectric tool was equal to 15 cells/mL in PBS or milk samples for 30 min. Also, in agreement with bacterial cell culture, the clinically infected milk samples by S. aureus and E. coli were successfully detected using the immunoelectric device. CONCLUSIONS: The developed immunoelectric device could be utilized for rapid and cost–benefit detection of the bacterial organism. John Wiley and Sons Inc. 2023-06-23 /pmc/articles/PMC10388230/ /pubmed/37352319 http://dx.doi.org/10.1002/jcla.24939 Text en © 2023 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Mohammad, Khosravi Meysam, Makki MohammadNasir, Ghoudarzi Darioush, Gharibi Detection of bacteria causing mastitis in cows with immunoelectric tool |
title | Detection of bacteria causing mastitis in cows with immunoelectric tool |
title_full | Detection of bacteria causing mastitis in cows with immunoelectric tool |
title_fullStr | Detection of bacteria causing mastitis in cows with immunoelectric tool |
title_full_unstemmed | Detection of bacteria causing mastitis in cows with immunoelectric tool |
title_short | Detection of bacteria causing mastitis in cows with immunoelectric tool |
title_sort | detection of bacteria causing mastitis in cows with immunoelectric tool |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388230/ https://www.ncbi.nlm.nih.gov/pubmed/37352319 http://dx.doi.org/10.1002/jcla.24939 |
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