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Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line

Bell's palsy is the most common form of facial nerve palsy. This study aimed to explore the pathogenesis of Bell's palsy by investigating the effect of cold-stimulated adipocyte supernatant on adhesion molecule expression in Schwann cell line. Schwann cells were cultured in regular or adip...

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Autores principales: Zhang, Tiefeng, Gan, Yaxin, Bai, Yulan, Hao, Weijiang, Zeng, Ziqi, Wu, Feng, Li, Xianqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388730/
https://www.ncbi.nlm.nih.gov/pubmed/37529013
http://dx.doi.org/10.1016/j.bbrep.2023.101523
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author Zhang, Tiefeng
Gan, Yaxin
Bai, Yulan
Hao, Weijiang
Zeng, Ziqi
Wu, Feng
Li, Xianqi
author_facet Zhang, Tiefeng
Gan, Yaxin
Bai, Yulan
Hao, Weijiang
Zeng, Ziqi
Wu, Feng
Li, Xianqi
author_sort Zhang, Tiefeng
collection PubMed
description Bell's palsy is the most common form of facial nerve palsy. This study aimed to explore the pathogenesis of Bell's palsy by investigating the effect of cold-stimulated adipocyte supernatant on adhesion molecule expression in Schwann cell line. Schwann cells were cultured in regular or adipocyte-conditioned medium and analyzed using RNA sequencing. The mRNA expression of Schwann cell adhesion molecules melanoma cell adhesion molecule (MCAM), protocadherin 9 (PCDH9), and intercellular cell adhesion molecule 1 (ICAM1) was determined using real-time reverse-transcription polymerase chain reaction. Differentially expressed genes were identified, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were conducted. Compared with Schwann cells in 37 °C, the expression of MCAM, PCDH9, and ICAM1 was downregulated in Schwann cells treated with cold-stimulated adipocyte supernatant compared with Schwann cells in 37 °C. Adipocytes subjected to cold exposure may weaken the adhesion capacity of Schwann cells and disrupt the local homeostasis of Schwann cell–axon interactions by affecting the expression of MCAM, PCDH9, and ICAM1, ultimately leading to the development of demyelinating lesions.
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spelling pubmed-103887302023-08-01 Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line Zhang, Tiefeng Gan, Yaxin Bai, Yulan Hao, Weijiang Zeng, Ziqi Wu, Feng Li, Xianqi Biochem Biophys Rep Research Article Bell's palsy is the most common form of facial nerve palsy. This study aimed to explore the pathogenesis of Bell's palsy by investigating the effect of cold-stimulated adipocyte supernatant on adhesion molecule expression in Schwann cell line. Schwann cells were cultured in regular or adipocyte-conditioned medium and analyzed using RNA sequencing. The mRNA expression of Schwann cell adhesion molecules melanoma cell adhesion molecule (MCAM), protocadherin 9 (PCDH9), and intercellular cell adhesion molecule 1 (ICAM1) was determined using real-time reverse-transcription polymerase chain reaction. Differentially expressed genes were identified, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were conducted. Compared with Schwann cells in 37 °C, the expression of MCAM, PCDH9, and ICAM1 was downregulated in Schwann cells treated with cold-stimulated adipocyte supernatant compared with Schwann cells in 37 °C. Adipocytes subjected to cold exposure may weaken the adhesion capacity of Schwann cells and disrupt the local homeostasis of Schwann cell–axon interactions by affecting the expression of MCAM, PCDH9, and ICAM1, ultimately leading to the development of demyelinating lesions. Elsevier 2023-07-28 /pmc/articles/PMC10388730/ /pubmed/37529013 http://dx.doi.org/10.1016/j.bbrep.2023.101523 Text en © 2023 Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Zhang, Tiefeng
Gan, Yaxin
Bai, Yulan
Hao, Weijiang
Zeng, Ziqi
Wu, Feng
Li, Xianqi
Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line
title Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line
title_full Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line
title_fullStr Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line
title_full_unstemmed Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line
title_short Influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in Schwann cell line
title_sort influence of cold-stimulated adipocyte supernatant on the expression of adhesion-related molecules in schwann cell line
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388730/
https://www.ncbi.nlm.nih.gov/pubmed/37529013
http://dx.doi.org/10.1016/j.bbrep.2023.101523
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