Cargando…

Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits

BACKGROUND: An arteriovenous fistula (AVF) is considered essential for chronic hemodialysis. OBJECTIVE: To determine the effects of hydraulic expansion on the intimal hyperplasia of an AVF. METHODS: We divided 12 healthy male New Zealand white rabbits into a control group (vein without special handl...

Descripción completa

Detalles Bibliográficos
Autores principales: Ren, Wanjun, Niu, Jiyuan, Du, Yuejuan, Jiang, Huili
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388758/
https://www.ncbi.nlm.nih.gov/pubmed/37551325
http://dx.doi.org/10.2478/abm-2021-0028
_version_ 1785082190731673600
author Ren, Wanjun
Niu, Jiyuan
Du, Yuejuan
Jiang, Huili
author_facet Ren, Wanjun
Niu, Jiyuan
Du, Yuejuan
Jiang, Huili
author_sort Ren, Wanjun
collection PubMed
description BACKGROUND: An arteriovenous fistula (AVF) is considered essential for chronic hemodialysis. OBJECTIVE: To determine the effects of hydraulic expansion on the intimal hyperplasia of an AVF. METHODS: We divided 12 healthy male New Zealand white rabbits into a control group (vein without special handling and direct anastomosis with an artery, n = 6) and a hydraulic expansion group (vein dilated by hydraulic pressure before anastomosis, n = 6). Histopathomorphology was examined with hematoxylin and eosin staining and immunohistochemistry. Analysis of covariance (ANCOVA) was used to compare the data between the groups. RESULTS: Immediately and 1 day after surgery, the diameter of the fistula vein in rabbits in the hydraulic expansion group was significantly larger than it was in the control group (P = 0.02 and 0.03 respectively), but not on subsequent days. After hydraulic expansion and before construction of the fistula, the wall of vein was noticeably thinner on macroscopic observation, and the anterior and posterior walls were indistinguishable. At 3 weeks after surgery in the hydraulic expansion group, cells in the vein wall were disordered, there were fewer elastic fibers, tissues from the endothelium to tunica externa were less dense, and there was less extracellular matrix than in the control group. Expression of connective tissue growth factor in the hydraulic expansion group was significantly less than that in the control group (P = 0.01). No differences were found in intimal thickness or immunohistochemistry scores for transforming growth factor-β1 between the groups. CONCLUSION: Hydraulic expansion did not increase intimal hyperplasia of an AVF, but facilitates remodeling of AVFs in rabbits.
format Online
Article
Text
id pubmed-10388758
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Sciendo
record_format MEDLINE/PubMed
spelling pubmed-103887582023-08-07 Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits Ren, Wanjun Niu, Jiyuan Du, Yuejuan Jiang, Huili Asian Biomed (Res Rev News) Original Article BACKGROUND: An arteriovenous fistula (AVF) is considered essential for chronic hemodialysis. OBJECTIVE: To determine the effects of hydraulic expansion on the intimal hyperplasia of an AVF. METHODS: We divided 12 healthy male New Zealand white rabbits into a control group (vein without special handling and direct anastomosis with an artery, n = 6) and a hydraulic expansion group (vein dilated by hydraulic pressure before anastomosis, n = 6). Histopathomorphology was examined with hematoxylin and eosin staining and immunohistochemistry. Analysis of covariance (ANCOVA) was used to compare the data between the groups. RESULTS: Immediately and 1 day after surgery, the diameter of the fistula vein in rabbits in the hydraulic expansion group was significantly larger than it was in the control group (P = 0.02 and 0.03 respectively), but not on subsequent days. After hydraulic expansion and before construction of the fistula, the wall of vein was noticeably thinner on macroscopic observation, and the anterior and posterior walls were indistinguishable. At 3 weeks after surgery in the hydraulic expansion group, cells in the vein wall were disordered, there were fewer elastic fibers, tissues from the endothelium to tunica externa were less dense, and there was less extracellular matrix than in the control group. Expression of connective tissue growth factor in the hydraulic expansion group was significantly less than that in the control group (P = 0.01). No differences were found in intimal thickness or immunohistochemistry scores for transforming growth factor-β1 between the groups. CONCLUSION: Hydraulic expansion did not increase intimal hyperplasia of an AVF, but facilitates remodeling of AVFs in rabbits. Sciendo 2021-10-29 /pmc/articles/PMC10388758/ /pubmed/37551325 http://dx.doi.org/10.2478/abm-2021-0028 Text en © 2021 Wanjun Ren et al., published by Sciendo https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Original Article
Ren, Wanjun
Niu, Jiyuan
Du, Yuejuan
Jiang, Huili
Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits
title Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits
title_full Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits
title_fullStr Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits
title_full_unstemmed Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits
title_short Hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits
title_sort hydraulic expansion facilitates remodeling of arteriovenous fistulas without increasing venous intimal hyperplasia in rabbits
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10388758/
https://www.ncbi.nlm.nih.gov/pubmed/37551325
http://dx.doi.org/10.2478/abm-2021-0028
work_keys_str_mv AT renwanjun hydraulicexpansionfacilitatesremodelingofarteriovenousfistulaswithoutincreasingvenousintimalhyperplasiainrabbits
AT niujiyuan hydraulicexpansionfacilitatesremodelingofarteriovenousfistulaswithoutincreasingvenousintimalhyperplasiainrabbits
AT duyuejuan hydraulicexpansionfacilitatesremodelingofarteriovenousfistulaswithoutincreasingvenousintimalhyperplasiainrabbits
AT jianghuili hydraulicexpansionfacilitatesremodelingofarteriovenousfistulaswithoutincreasingvenousintimalhyperplasiainrabbits