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Integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in Oenanthe javanica

Oenanthe javanica (Blume) DC. is a popular vegetable with unique flavor and its leaf is the main product organ. Gibberellin (GA) is an important plant hormone that plays vital roles in regulating the growth of plants. In this study, the plants of water dropwort were treated with different concentrat...

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Autores principales: Feng, Kai, Li, Xibei, Yan, Yajie, Liu, Ruozhenyi, Li, Zixuan, Sun, Nan, Yang, Zhiyuan, Zhao, Shuping, Wu, Peng, Li, Liangjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389089/
https://www.ncbi.nlm.nih.gov/pubmed/37528973
http://dx.doi.org/10.3389/fpls.2023.1225635
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author Feng, Kai
Li, Xibei
Yan, Yajie
Liu, Ruozhenyi
Li, Zixuan
Sun, Nan
Yang, Zhiyuan
Zhao, Shuping
Wu, Peng
Li, Liangjun
author_facet Feng, Kai
Li, Xibei
Yan, Yajie
Liu, Ruozhenyi
Li, Zixuan
Sun, Nan
Yang, Zhiyuan
Zhao, Shuping
Wu, Peng
Li, Liangjun
author_sort Feng, Kai
collection PubMed
description Oenanthe javanica (Blume) DC. is a popular vegetable with unique flavor and its leaf is the main product organ. Gibberellin (GA) is an important plant hormone that plays vital roles in regulating the growth of plants. In this study, the plants of water dropwort were treated with different concentrations of GA(3). The plant height of water dropwort was significantly increased after GA(3) treatment. Anatomical structure analysis indicated that the cell length of water dropwort was elongated under exogenous application of GA(3). The metabolome analysis showed flavonoids were the most abundant metabolites and the biosynthesis of secondary metabolites were also regulated by GA(3). The exogenous application of GA(3) altered the gene expressions of plant hormone signal transduction (GID and DELLA) and metabolites biosynthesis pathways to regulate the growth of water dropwort. The GA contents were modulated by up-regulating the expression of GA metabolism gene GA2ox. The differentially expressed genes related to cell wall formation were significantly enriched. A total of 22 cellulose synthase involved in cellulose biosynthesis were identified from the genome of water dropwort. Our results indicated that GA treatment promoted the cell elongation by inducing the expression of cellulose synthase and cell wall formation in water dropwort. These results revealed the molecular mechanism of GA-mediated cell elongation, which will provide valuable reference for using GA to regulate the growth of water dropwort.
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spelling pubmed-103890892023-08-01 Integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in Oenanthe javanica Feng, Kai Li, Xibei Yan, Yajie Liu, Ruozhenyi Li, Zixuan Sun, Nan Yang, Zhiyuan Zhao, Shuping Wu, Peng Li, Liangjun Front Plant Sci Plant Science Oenanthe javanica (Blume) DC. is a popular vegetable with unique flavor and its leaf is the main product organ. Gibberellin (GA) is an important plant hormone that plays vital roles in regulating the growth of plants. In this study, the plants of water dropwort were treated with different concentrations of GA(3). The plant height of water dropwort was significantly increased after GA(3) treatment. Anatomical structure analysis indicated that the cell length of water dropwort was elongated under exogenous application of GA(3). The metabolome analysis showed flavonoids were the most abundant metabolites and the biosynthesis of secondary metabolites were also regulated by GA(3). The exogenous application of GA(3) altered the gene expressions of plant hormone signal transduction (GID and DELLA) and metabolites biosynthesis pathways to regulate the growth of water dropwort. The GA contents were modulated by up-regulating the expression of GA metabolism gene GA2ox. The differentially expressed genes related to cell wall formation were significantly enriched. A total of 22 cellulose synthase involved in cellulose biosynthesis were identified from the genome of water dropwort. Our results indicated that GA treatment promoted the cell elongation by inducing the expression of cellulose synthase and cell wall formation in water dropwort. These results revealed the molecular mechanism of GA-mediated cell elongation, which will provide valuable reference for using GA to regulate the growth of water dropwort. Frontiers Media S.A. 2023-07-17 /pmc/articles/PMC10389089/ /pubmed/37528973 http://dx.doi.org/10.3389/fpls.2023.1225635 Text en Copyright © 2023 Feng, Li, Yan, Liu, Li, Sun, Yang, Zhao, Wu and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Feng, Kai
Li, Xibei
Yan, Yajie
Liu, Ruozhenyi
Li, Zixuan
Sun, Nan
Yang, Zhiyuan
Zhao, Shuping
Wu, Peng
Li, Liangjun
Integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in Oenanthe javanica
title Integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in Oenanthe javanica
title_full Integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in Oenanthe javanica
title_fullStr Integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in Oenanthe javanica
title_full_unstemmed Integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in Oenanthe javanica
title_short Integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in Oenanthe javanica
title_sort integrated morphological, metabolome, and transcriptome analyses revealed the mechanism of exogenous gibberellin promoting petiole elongation in oenanthe javanica
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389089/
https://www.ncbi.nlm.nih.gov/pubmed/37528973
http://dx.doi.org/10.3389/fpls.2023.1225635
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