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Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems
Excess phosphorus (P) in wastewater effluent poses a serious threat to aquatic ecosystems and can spur harmful algal blooms. Revolving algal biofilm (RAB) systems are an emerging technology to recover P from wastewater before discharge into aquatic ecosystems. In RAB systems, a community of microalg...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389661/ https://www.ncbi.nlm.nih.gov/pubmed/37529323 http://dx.doi.org/10.3389/fmicb.2023.1219318 |
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author | Schaedig, Eric Cantrell, Michael Urban, Chris Zhao, Xuefei Greene, Drew Dancer, Jens Gross, Michael Sebesta, Jacob Chou, Katherine J. Grabowy, Jonathan Gross, Martin Kumar, Kuldip Yu, Jianping |
author_facet | Schaedig, Eric Cantrell, Michael Urban, Chris Zhao, Xuefei Greene, Drew Dancer, Jens Gross, Michael Sebesta, Jacob Chou, Katherine J. Grabowy, Jonathan Gross, Martin Kumar, Kuldip Yu, Jianping |
author_sort | Schaedig, Eric |
collection | PubMed |
description | Excess phosphorus (P) in wastewater effluent poses a serious threat to aquatic ecosystems and can spur harmful algal blooms. Revolving algal biofilm (RAB) systems are an emerging technology to recover P from wastewater before discharge into aquatic ecosystems. In RAB systems, a community of microalgae take up and store wastewater P as polyphosphate as they grow in a partially submerged revolving biofilm, which may then be harvested and dried for use as fertilizer in lieu of mined phosphate rock. In this work, we isolated and characterized a total of 101 microalgae strains from active RAB systems across the US Midwest, including 82 green algae, 9 diatoms, and 10 cyanobacteria. Strains were identified by microscopy and 16S/18S ribosomal DNA sequencing, cryopreserved, and screened for elevated P content (as polyphosphate). Seven isolated strains possessed at least 50% more polyphosphate by cell dry weight than a microalgae consortium from a RAB system, with the top strain accumulating nearly threefold more polyphosphate. These top P-hyperaccumulating strains include the green alga Chlamydomonas pulvinata TCF-48 g and the diatoms Eolimna minima TCF-3d and Craticula molestiformis TCF-8d, possessing 11.4, 12.7, and 14.0% polyphosphate by cell dry weight, respectively. As a preliminary test of strain application for recovering P, Chlamydomonas pulvinata TCF-48 g was reinoculated into a bench-scale RAB system containing Bold basal medium. The strain successfully recolonized the system and recovered twofold more P from the medium than a microalgae consortium from a RAB system treating municipal wastewater. These isolated P-hyperaccumulating microalgae may have broad applications in resource recovery from various waste streams, including improving P removal from wastewater. |
format | Online Article Text |
id | pubmed-10389661 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-103896612023-08-01 Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems Schaedig, Eric Cantrell, Michael Urban, Chris Zhao, Xuefei Greene, Drew Dancer, Jens Gross, Michael Sebesta, Jacob Chou, Katherine J. Grabowy, Jonathan Gross, Martin Kumar, Kuldip Yu, Jianping Front Microbiol Microbiology Excess phosphorus (P) in wastewater effluent poses a serious threat to aquatic ecosystems and can spur harmful algal blooms. Revolving algal biofilm (RAB) systems are an emerging technology to recover P from wastewater before discharge into aquatic ecosystems. In RAB systems, a community of microalgae take up and store wastewater P as polyphosphate as they grow in a partially submerged revolving biofilm, which may then be harvested and dried for use as fertilizer in lieu of mined phosphate rock. In this work, we isolated and characterized a total of 101 microalgae strains from active RAB systems across the US Midwest, including 82 green algae, 9 diatoms, and 10 cyanobacteria. Strains were identified by microscopy and 16S/18S ribosomal DNA sequencing, cryopreserved, and screened for elevated P content (as polyphosphate). Seven isolated strains possessed at least 50% more polyphosphate by cell dry weight than a microalgae consortium from a RAB system, with the top strain accumulating nearly threefold more polyphosphate. These top P-hyperaccumulating strains include the green alga Chlamydomonas pulvinata TCF-48 g and the diatoms Eolimna minima TCF-3d and Craticula molestiformis TCF-8d, possessing 11.4, 12.7, and 14.0% polyphosphate by cell dry weight, respectively. As a preliminary test of strain application for recovering P, Chlamydomonas pulvinata TCF-48 g was reinoculated into a bench-scale RAB system containing Bold basal medium. The strain successfully recolonized the system and recovered twofold more P from the medium than a microalgae consortium from a RAB system treating municipal wastewater. These isolated P-hyperaccumulating microalgae may have broad applications in resource recovery from various waste streams, including improving P removal from wastewater. Frontiers Media S.A. 2023-07-17 /pmc/articles/PMC10389661/ /pubmed/37529323 http://dx.doi.org/10.3389/fmicb.2023.1219318 Text en Copyright © 2023 Schaedig, Cantrell, Urban, Zhao, Greene, Dancer, Gross, Sebesta, Chou, Grabowy, Gross, Kumar and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Schaedig, Eric Cantrell, Michael Urban, Chris Zhao, Xuefei Greene, Drew Dancer, Jens Gross, Michael Sebesta, Jacob Chou, Katherine J. Grabowy, Jonathan Gross, Martin Kumar, Kuldip Yu, Jianping Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems |
title | Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems |
title_full | Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems |
title_fullStr | Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems |
title_full_unstemmed | Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems |
title_short | Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems |
title_sort | isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (rab) wastewater treatment systems |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389661/ https://www.ncbi.nlm.nih.gov/pubmed/37529323 http://dx.doi.org/10.3389/fmicb.2023.1219318 |
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