Cargando…

Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems

Excess phosphorus (P) in wastewater effluent poses a serious threat to aquatic ecosystems and can spur harmful algal blooms. Revolving algal biofilm (RAB) systems are an emerging technology to recover P from wastewater before discharge into aquatic ecosystems. In RAB systems, a community of microalg...

Descripción completa

Detalles Bibliográficos
Autores principales: Schaedig, Eric, Cantrell, Michael, Urban, Chris, Zhao, Xuefei, Greene, Drew, Dancer, Jens, Gross, Michael, Sebesta, Jacob, Chou, Katherine J., Grabowy, Jonathan, Gross, Martin, Kumar, Kuldip, Yu, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389661/
https://www.ncbi.nlm.nih.gov/pubmed/37529323
http://dx.doi.org/10.3389/fmicb.2023.1219318
_version_ 1785082352352886784
author Schaedig, Eric
Cantrell, Michael
Urban, Chris
Zhao, Xuefei
Greene, Drew
Dancer, Jens
Gross, Michael
Sebesta, Jacob
Chou, Katherine J.
Grabowy, Jonathan
Gross, Martin
Kumar, Kuldip
Yu, Jianping
author_facet Schaedig, Eric
Cantrell, Michael
Urban, Chris
Zhao, Xuefei
Greene, Drew
Dancer, Jens
Gross, Michael
Sebesta, Jacob
Chou, Katherine J.
Grabowy, Jonathan
Gross, Martin
Kumar, Kuldip
Yu, Jianping
author_sort Schaedig, Eric
collection PubMed
description Excess phosphorus (P) in wastewater effluent poses a serious threat to aquatic ecosystems and can spur harmful algal blooms. Revolving algal biofilm (RAB) systems are an emerging technology to recover P from wastewater before discharge into aquatic ecosystems. In RAB systems, a community of microalgae take up and store wastewater P as polyphosphate as they grow in a partially submerged revolving biofilm, which may then be harvested and dried for use as fertilizer in lieu of mined phosphate rock. In this work, we isolated and characterized a total of 101 microalgae strains from active RAB systems across the US Midwest, including 82 green algae, 9 diatoms, and 10 cyanobacteria. Strains were identified by microscopy and 16S/18S ribosomal DNA sequencing, cryopreserved, and screened for elevated P content (as polyphosphate). Seven isolated strains possessed at least 50% more polyphosphate by cell dry weight than a microalgae consortium from a RAB system, with the top strain accumulating nearly threefold more polyphosphate. These top P-hyperaccumulating strains include the green alga Chlamydomonas pulvinata TCF-48 g and the diatoms Eolimna minima TCF-3d and Craticula molestiformis TCF-8d, possessing 11.4, 12.7, and 14.0% polyphosphate by cell dry weight, respectively. As a preliminary test of strain application for recovering P, Chlamydomonas pulvinata TCF-48 g was reinoculated into a bench-scale RAB system containing Bold basal medium. The strain successfully recolonized the system and recovered twofold more P from the medium than a microalgae consortium from a RAB system treating municipal wastewater. These isolated P-hyperaccumulating microalgae may have broad applications in resource recovery from various waste streams, including improving P removal from wastewater.
format Online
Article
Text
id pubmed-10389661
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-103896612023-08-01 Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems Schaedig, Eric Cantrell, Michael Urban, Chris Zhao, Xuefei Greene, Drew Dancer, Jens Gross, Michael Sebesta, Jacob Chou, Katherine J. Grabowy, Jonathan Gross, Martin Kumar, Kuldip Yu, Jianping Front Microbiol Microbiology Excess phosphorus (P) in wastewater effluent poses a serious threat to aquatic ecosystems and can spur harmful algal blooms. Revolving algal biofilm (RAB) systems are an emerging technology to recover P from wastewater before discharge into aquatic ecosystems. In RAB systems, a community of microalgae take up and store wastewater P as polyphosphate as they grow in a partially submerged revolving biofilm, which may then be harvested and dried for use as fertilizer in lieu of mined phosphate rock. In this work, we isolated and characterized a total of 101 microalgae strains from active RAB systems across the US Midwest, including 82 green algae, 9 diatoms, and 10 cyanobacteria. Strains were identified by microscopy and 16S/18S ribosomal DNA sequencing, cryopreserved, and screened for elevated P content (as polyphosphate). Seven isolated strains possessed at least 50% more polyphosphate by cell dry weight than a microalgae consortium from a RAB system, with the top strain accumulating nearly threefold more polyphosphate. These top P-hyperaccumulating strains include the green alga Chlamydomonas pulvinata TCF-48 g and the diatoms Eolimna minima TCF-3d and Craticula molestiformis TCF-8d, possessing 11.4, 12.7, and 14.0% polyphosphate by cell dry weight, respectively. As a preliminary test of strain application for recovering P, Chlamydomonas pulvinata TCF-48 g was reinoculated into a bench-scale RAB system containing Bold basal medium. The strain successfully recolonized the system and recovered twofold more P from the medium than a microalgae consortium from a RAB system treating municipal wastewater. These isolated P-hyperaccumulating microalgae may have broad applications in resource recovery from various waste streams, including improving P removal from wastewater. Frontiers Media S.A. 2023-07-17 /pmc/articles/PMC10389661/ /pubmed/37529323 http://dx.doi.org/10.3389/fmicb.2023.1219318 Text en Copyright © 2023 Schaedig, Cantrell, Urban, Zhao, Greene, Dancer, Gross, Sebesta, Chou, Grabowy, Gross, Kumar and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Schaedig, Eric
Cantrell, Michael
Urban, Chris
Zhao, Xuefei
Greene, Drew
Dancer, Jens
Gross, Michael
Sebesta, Jacob
Chou, Katherine J.
Grabowy, Jonathan
Gross, Martin
Kumar, Kuldip
Yu, Jianping
Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems
title Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems
title_full Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems
title_fullStr Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems
title_full_unstemmed Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems
title_short Isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (RAB) wastewater treatment systems
title_sort isolation of phosphorus-hyperaccumulating microalgae from revolving algal biofilm (rab) wastewater treatment systems
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389661/
https://www.ncbi.nlm.nih.gov/pubmed/37529323
http://dx.doi.org/10.3389/fmicb.2023.1219318
work_keys_str_mv AT schaedigeric isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT cantrellmichael isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT urbanchris isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT zhaoxuefei isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT greenedrew isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT dancerjens isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT grossmichael isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT sebestajacob isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT choukatherinej isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT grabowyjonathan isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT grossmartin isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT kumarkuldip isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems
AT yujianping isolationofphosphorushyperaccumulatingmicroalgaefromrevolvingalgalbiofilmrabwastewatertreatmentsystems