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Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.)

Rice (Oryza sativa L.) is a staple food in many countries around the world, particularly in China. The production of rice is seriously affected by the bacterial leaf streak and rice blast, which can reduce rice yield or even cause it to fail to be harvested. In this study, susceptible material 58B w...

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Autores principales: Yang, Jinlian, Fang, Yaoyu, Wu, Hu, Zhao, Neng, Guo, Xinying, Mackon, Enerand, Peng, Haowen, Huang, Sheng, He, Yongqiang, Qin, Baoxiang, Liu, Yaoguang, Liu, Fang, Chen, Shengwu, Li, Rongbai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389665/
https://www.ncbi.nlm.nih.gov/pubmed/37528980
http://dx.doi.org/10.3389/fpls.2023.1209384
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author Yang, Jinlian
Fang, Yaoyu
Wu, Hu
Zhao, Neng
Guo, Xinying
Mackon, Enerand
Peng, Haowen
Huang, Sheng
He, Yongqiang
Qin, Baoxiang
Liu, Yaoguang
Liu, Fang
Chen, Shengwu
Li, Rongbai
author_facet Yang, Jinlian
Fang, Yaoyu
Wu, Hu
Zhao, Neng
Guo, Xinying
Mackon, Enerand
Peng, Haowen
Huang, Sheng
He, Yongqiang
Qin, Baoxiang
Liu, Yaoguang
Liu, Fang
Chen, Shengwu
Li, Rongbai
author_sort Yang, Jinlian
collection PubMed
description Rice (Oryza sativa L.) is a staple food in many countries around the world, particularly in China. The production of rice is seriously affected by the bacterial leaf streak and rice blast, which can reduce rice yield or even cause it to fail to be harvested. In this study, susceptible material 58B was edited by CRISPR/Cas9, targeting a target of the Pi21 gene and a target of the effector-binding element (EBE) of the OsSULTR3;6 gene, and the mutants 58b were obtained by Agrobacterium-mediated method. The editing efficiency of the two targets in the T(0) generation was higher than 90.09%, the homozygous mutants were successfully selected in the T(0) generation, and the homozygous mutation rate of each target was higher than 26.67%. The expression of the edited pi21 and EBE of Ossultr3;6 was significantly reduced, and the expression of defense responsive genes was significantly upregulated after infected with rice blast. The lesion areas of rice blast and bacterial leaf streak were significantly reduced in 58b, and the resistance of both was effectively improved. Furthermore, the gene editing events did not affect the agronomic traits of rice. In this study, the resistance of 58b to rice blast and bacterial leaf streak was improved simultaneously. This study provides a reference for using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 (CRISPR/Cas9) to accelerate the improvement of rice varieties and the development of new materials for rice breeding.
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spelling pubmed-103896652023-08-01 Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.) Yang, Jinlian Fang, Yaoyu Wu, Hu Zhao, Neng Guo, Xinying Mackon, Enerand Peng, Haowen Huang, Sheng He, Yongqiang Qin, Baoxiang Liu, Yaoguang Liu, Fang Chen, Shengwu Li, Rongbai Front Plant Sci Plant Science Rice (Oryza sativa L.) is a staple food in many countries around the world, particularly in China. The production of rice is seriously affected by the bacterial leaf streak and rice blast, which can reduce rice yield or even cause it to fail to be harvested. In this study, susceptible material 58B was edited by CRISPR/Cas9, targeting a target of the Pi21 gene and a target of the effector-binding element (EBE) of the OsSULTR3;6 gene, and the mutants 58b were obtained by Agrobacterium-mediated method. The editing efficiency of the two targets in the T(0) generation was higher than 90.09%, the homozygous mutants were successfully selected in the T(0) generation, and the homozygous mutation rate of each target was higher than 26.67%. The expression of the edited pi21 and EBE of Ossultr3;6 was significantly reduced, and the expression of defense responsive genes was significantly upregulated after infected with rice blast. The lesion areas of rice blast and bacterial leaf streak were significantly reduced in 58b, and the resistance of both was effectively improved. Furthermore, the gene editing events did not affect the agronomic traits of rice. In this study, the resistance of 58b to rice blast and bacterial leaf streak was improved simultaneously. This study provides a reference for using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 (CRISPR/Cas9) to accelerate the improvement of rice varieties and the development of new materials for rice breeding. Frontiers Media S.A. 2023-07-17 /pmc/articles/PMC10389665/ /pubmed/37528980 http://dx.doi.org/10.3389/fpls.2023.1209384 Text en Copyright © 2023 Yang, Fang, Wu, Zhao, Guo, Mackon, Peng, Huang, He, Qin, Liu, Liu, Chen and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yang, Jinlian
Fang, Yaoyu
Wu, Hu
Zhao, Neng
Guo, Xinying
Mackon, Enerand
Peng, Haowen
Huang, Sheng
He, Yongqiang
Qin, Baoxiang
Liu, Yaoguang
Liu, Fang
Chen, Shengwu
Li, Rongbai
Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.)
title Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.)
title_full Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.)
title_fullStr Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.)
title_full_unstemmed Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.)
title_short Improvement of resistance to rice blast and bacterial leaf streak by CRISPR/Cas9-mediated mutagenesis of Pi21 and OsSULTR3;6 in rice (Oryza sativa L.)
title_sort improvement of resistance to rice blast and bacterial leaf streak by crispr/cas9-mediated mutagenesis of pi21 and ossultr3;6 in rice (oryza sativa l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389665/
https://www.ncbi.nlm.nih.gov/pubmed/37528980
http://dx.doi.org/10.3389/fpls.2023.1209384
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