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Enzyme-based assay for quantification of UDP-GlcNAc in cells and tissues

In this issue of Cell Reports Methods, Sunden et al. develop an enzymatic assay to measure UDP-GlcNAc levels from cells and tissue.(1) By reporting on the level of the substrate itself, this approach can potentially enhance the fields’ understanding of UDP-GlcNAc concentration under a variety of con...

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Detalles Bibliográficos
Autores principales: Chatham, John C., Ha, Chae-Myeong, Wende, Adam R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391555/
https://www.ncbi.nlm.nih.gov/pubmed/37533649
http://dx.doi.org/10.1016/j.crmeth.2023.100537
Descripción
Sumario:In this issue of Cell Reports Methods, Sunden et al. develop an enzymatic assay to measure UDP-GlcNAc levels from cells and tissue.(1) By reporting on the level of the substrate itself, this approach can potentially enhance the fields’ understanding of UDP-GlcNAc concentration under a variety of conditions.