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A structured evaluation of cryopreservation in generating single-cell transcriptomes from cerebrospinal fluid

Single-cell transcriptomics allows characterization of cerebrospinal fluid (CSF) cells at an unprecedented level. Here, we report a robust cryopreservation protocol adapted for the characterization of fragile CSF cells by single-cell RNA sequencing (RNA-seq) in moderate- to large-scale studies. Fres...

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Detalles Bibliográficos
Autores principales: Touil, Hanane, Roostaei, Tina, Calini, Daniela, Diaconu, Claudiu, Epstein, Samantha, Raposo, Catarina, Onomichi, Kaho, Thakur, Kiran T., Craveiro, Licinio, Callegari, Ilaria, Bryois, Julien, Riley, Claire S., Menon, Vilas, Derfuss, Tobias, De Jager, Philip L., Malhotra, Dheeraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391561/
https://www.ncbi.nlm.nih.gov/pubmed/37533636
http://dx.doi.org/10.1016/j.crmeth.2023.100533

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