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A structured evaluation of cryopreservation in generating single-cell transcriptomes from cerebrospinal fluid
Single-cell transcriptomics allows characterization of cerebrospinal fluid (CSF) cells at an unprecedented level. Here, we report a robust cryopreservation protocol adapted for the characterization of fragile CSF cells by single-cell RNA sequencing (RNA-seq) in moderate- to large-scale studies. Fres...
Autores principales: | Touil, Hanane, Roostaei, Tina, Calini, Daniela, Diaconu, Claudiu, Epstein, Samantha, Raposo, Catarina, Onomichi, Kaho, Thakur, Kiran T., Craveiro, Licinio, Callegari, Ilaria, Bryois, Julien, Riley, Claire S., Menon, Vilas, Derfuss, Tobias, De Jager, Philip L., Malhotra, Dheeraj |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391561/ https://www.ncbi.nlm.nih.gov/pubmed/37533636 http://dx.doi.org/10.1016/j.crmeth.2023.100533 |
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