Cargando…

A real-time fluorescent gp32 probe-based assay for monitoring single-stranded DNA-dependent DNA processing enzymes

Single-stranded DNA (ssDNA) generated during DNA replication, recombination and damage repair reactions is an important intermediate and ssDNA-binding proteins that binds these intermediates coordinate various DNA metabolic processes. Mechanistic details of these ssDNA-dependent processes can be exp...

Descripción completa

Detalles Bibliográficos
Autor principal:	Perumal, Senthil K.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391720/ https://www.ncbi.nlm.nih.gov/pubmed/37534323 http://dx.doi.org/10.1016/j.bbrep.2023.101518

Ejemplares similares

Mapping the interactions of the single-stranded DNA binding protein of bacteriophage T4 (gp32) with DNA lattices at single nucleotide resolution: gp32 monomer binding
por: Jose, Davis, et al.
Publicado: (2015)

Fluorescent probes for the analysis of DNA strand scission in base excision repair
por: Matsumoto, Naoyuki, et al.
Publicado: (2010)

Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA
por: Lietard, Jory, et al.
Publicado: (2022)

G-quadruplex formation in double strand DNA probed by NMM and CV fluorescence
por: Kreig, Alex, et al.
Publicado: (2015)

Mapping the interactions of the single-stranded DNA binding protein of bacteriophage T4 (gp32) with DNA lattices at single nucleotide resolution: polynucleotide binding and cooperativity
por: Jose, Davis, et al.
Publicado: (2015)

Mapping DNA conformations and interactions within the binding cleft of bacteriophage T4 single-stranded DNA binding protein (gp32) at single nucleotide resolution
por: Camel, Benjamin R, et al.
Publicado: (2020)

Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe
por: Zhang, Suge, et al.
Publicado: (2018)

A Sensitive DNA Enzyme-Based Fluorescent Assay for Bacterial Detection
por: Aguirre, Sergio D., et al.
Publicado: (2013)

Multiplex target capture with double-stranded DNA probes
por: Shen, Peidong, et al.
Publicado: (2013)

ctDNA Detection Based on DNA Clutch Probes and Strand Exchange Mechanism
por: Chang, Huan, et al.
Publicado: (2018)

Nanorings to Probe Mechanical Stress of Single-Stranded DNA Mediated by the DNA Duplex
por: Zagorski, Karen, et al.
Publicado: (2022)

Single-molecule FRET studies of the cooperative and non-cooperative binding kinetics of the bacteriophage T4 single-stranded DNA binding protein (gp32) to ssDNA lattices at replication fork junctions
por: Lee, Wonbae, et al.
Publicado: (2016)

Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplification
por: Yi, Jizu, et al.
Publicado: (2006)

Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled Double-Stranded DNA
por: Kretschy, Nicole, et al.
Publicado: (2016)

Fluorescent Probes Detecting the Phagocytic Phase of Apoptosis: Enzyme-Substrate Complexes of Topoisomerase and DNA
por: Minchew, Candace L., et al.
Publicado: (2011)

Fluorescent single-stranded DNA-binding protein from Plasmodium falciparum as a biosensor for single-stranded DNA
por: Chisty, Liisa T., et al.
Publicado: (2018)

Niosome-Assisted Delivery of DNA Fluorescent Probe with Optimized Strand Displacement for Intracellular MicroRNA21 Imaging
por: Zhu, Zongwei, et al.
Publicado: (2022)

Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR
por: Huang, Shihai, et al.
Publicado: (2007)

Force-activated DNA substrates for probing individual proteins interacting with single-stranded DNA
por: Okoniewski, Stephen R., et al.
Publicado: (2017)

Enzyme-Free Detection of Mutations in Cancer DNA Using Synthetic Oligonucleotide Probes and Fluorescence Microscopy
por: Miotke, Laura, et al.
Publicado: (2015)

Phenanthridine–pyrene conjugates as fluorescent probes for DNA/RNA and an inactive mutant of dipeptidyl peptidase enzyme
por: Matić, Josipa, et al.
Publicado: (2023)

DNA Unwinding by Ring-Shaped T4 Helicase gp41 Is Hindered by Tension on the Occluded Strand
por: Ribeck, Noah, et al.
Publicado: (2013)

Performance Characteristics of Different Anti-Double-Stranded DNA Antibody Assays in the Monitoring of Systemic Lupus Erythematosus
por: Mahler, Michael, et al.
Publicado: (2017)

Highly Sensitive Fluorescence Assay for miRNA Detection: Investigation of the DNA Spacer Effect on the DSN Enzyme Activity toward Magnetic-Bead-Tethered Probes
por: Djebbi, Khouloud, et al.
Publicado: (2022)

Orthogonal Enzyme-Driven Timers for DNA Strand Displacement Reactions
por: Bucci, Juliette, et al.
Publicado: (2022)

DNA assay based on Nanoceria as Fluorescence Quenchers (NanoCeracQ DNA assay)
por: Bülbül, Gonca, et al.
Publicado: (2018)

Fabrication of Unimolecular Double-stranded DNA Microarrays on Solid Surfaces for Probing DNA-Protein/Drug Interactions
por: Wang, Jinke, et al.
Publicado: (2003)

A Fluorescent Probe to Measure DNA Damage and Repair
por: Condie, Allison G., et al.
Publicado: (2015)

Nanostructure and Corresponding Quenching Efficiency of Fluorescent DNA Probes
por: Guo, Wenjuan, et al.
Publicado: (2018)

DNA-Dye-Conjugates: Conformations and Spectra of Fluorescence Probes
por: Beierlein, Frank R., et al.
Publicado: (2016)

Characterization of the T4 gp32–ssDNA complex by native, cross-linking, and ultraviolet photodissociation mass spectrometry
por: Blevins, Molly S., et al.
Publicado: (2021)

A scalable CRISPR/Cas9-based fluorescent reporter assay to study DNA double-strand break repair choice
por: Roidos, Paris, et al.
Publicado: (2020)

A robust CRISPR–Cas9-based fluorescent reporter assay for the detection and quantification of DNA double-strand break repair
por: Eki, Rebeka, et al.
Publicado: (2020)

Detecting RNA-protein proximity at DNA double-strand breaks using combined fluorescence in situ hybridization with proximity ligation assay
por: Alagia, Adele, et al.
Publicado: (2023)

Single-Stranded DNA Catalyzes Hybridization of PCR-Products to Microarray Capture Probes
por: Dally, Simon, et al.
Publicado: (2014)

Real-time imaging of sulfhydryl single-stranded DNA aggregation
por: Zeng, Fanwei, et al.
Publicado: (2023)

Sequence dependence of electron-induced DNA strand breakage revealed by DNA nanoarrays
por: Keller, Adrian, et al.
Publicado: (2014)

Fluorescence Imaging Using Enzyme-Activatable Probes for Real-Time Identification of Pancreatic Cancer
por: Takahashi, Ryugen, et al.
Publicado: (2021)

Development of a 32P-postlabeling assay for 7-methylguanines in human DNA.
por: Mustonen, R, et al.
Publicado: (1993)

Development of a single-stranded DNA-binding protein fluorescent fusion toolbox
por: Dubiel, Katarzyna, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_j4jq2o7bv5mbr8n3jrejufpo98