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Evolutional insights into the interaction between Rab7 and RILP in lysosome motility
Lysosome motility is critical for the cellular function. However, Rab7-related transport elements showed genetic differences between vertebrates and invertebrates, making the mechanism of lysosomal motility mysterious. We suggested that Rab7 interacted with RILP as a feature of highly evolved organi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391735/ https://www.ncbi.nlm.nih.gov/pubmed/37534141 http://dx.doi.org/10.1016/j.isci.2023.107040 |
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author | Cui, Gaofeng Jiang, Zhiyan Chen, Yaoyao Li, Yun Ai, Shupei Sun, Ranran Yi, Xin Zhong, Guohua |
author_facet | Cui, Gaofeng Jiang, Zhiyan Chen, Yaoyao Li, Yun Ai, Shupei Sun, Ranran Yi, Xin Zhong, Guohua |
author_sort | Cui, Gaofeng |
collection | PubMed |
description | Lysosome motility is critical for the cellular function. However, Rab7-related transport elements showed genetic differences between vertebrates and invertebrates, making the mechanism of lysosomal motility mysterious. We suggested that Rab7 interacted with RILP as a feature of highly evolved organisms since they could interact with each other in Spodoptera frugiperda but not in Drosophila melanogaster. The N-terminus of Sf-RILP was identified to be necessary for their interaction, and Glu61 was supposed to be the key point for the stability of the interaction. A GC-rich domain on the C-terminal parts of Sf-RILP hampered the expression of Sf-RILP and its interaction with Sf-Rab7. Although the corresponding vital amino acids in the mammalian model at the C-terminus of Sf-RILP turned to be neutral, the C-terminus would also help with the homologous interactions between RILP fragments in insects. The significantly different interactions in invertebrates shed light on the biodiversity and complexity of lysosomal motility. |
format | Online Article Text |
id | pubmed-10391735 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103917352023-08-02 Evolutional insights into the interaction between Rab7 and RILP in lysosome motility Cui, Gaofeng Jiang, Zhiyan Chen, Yaoyao Li, Yun Ai, Shupei Sun, Ranran Yi, Xin Zhong, Guohua iScience Article Lysosome motility is critical for the cellular function. However, Rab7-related transport elements showed genetic differences between vertebrates and invertebrates, making the mechanism of lysosomal motility mysterious. We suggested that Rab7 interacted with RILP as a feature of highly evolved organisms since they could interact with each other in Spodoptera frugiperda but not in Drosophila melanogaster. The N-terminus of Sf-RILP was identified to be necessary for their interaction, and Glu61 was supposed to be the key point for the stability of the interaction. A GC-rich domain on the C-terminal parts of Sf-RILP hampered the expression of Sf-RILP and its interaction with Sf-Rab7. Although the corresponding vital amino acids in the mammalian model at the C-terminus of Sf-RILP turned to be neutral, the C-terminus would also help with the homologous interactions between RILP fragments in insects. The significantly different interactions in invertebrates shed light on the biodiversity and complexity of lysosomal motility. Elsevier 2023-06-07 /pmc/articles/PMC10391735/ /pubmed/37534141 http://dx.doi.org/10.1016/j.isci.2023.107040 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Cui, Gaofeng Jiang, Zhiyan Chen, Yaoyao Li, Yun Ai, Shupei Sun, Ranran Yi, Xin Zhong, Guohua Evolutional insights into the interaction between Rab7 and RILP in lysosome motility |
title | Evolutional insights into the interaction between Rab7 and RILP in lysosome motility |
title_full | Evolutional insights into the interaction between Rab7 and RILP in lysosome motility |
title_fullStr | Evolutional insights into the interaction between Rab7 and RILP in lysosome motility |
title_full_unstemmed | Evolutional insights into the interaction between Rab7 and RILP in lysosome motility |
title_short | Evolutional insights into the interaction between Rab7 and RILP in lysosome motility |
title_sort | evolutional insights into the interaction between rab7 and rilp in lysosome motility |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391735/ https://www.ncbi.nlm.nih.gov/pubmed/37534141 http://dx.doi.org/10.1016/j.isci.2023.107040 |
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