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Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake
In the present study, clover-grass press cake was treated by liquid hot water at temperatures of 180–200 °C for a reaction time of 5–10 min. Evaluation of pretreatments was based on the monosaccharide yield after enzymatic hydrolysis of the pretreated slurry and solid fraction, respectively. Extract...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391941/ https://www.ncbi.nlm.nih.gov/pubmed/37534002 http://dx.doi.org/10.1016/j.heliyon.2023.e18448 |
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author | Bekker, Nicolai Sundgaard Toor, Saqib Sohail Sharma, Kamaldeep Pedersen, Thomas Helmer Pedersen, Lars Haastrup |
author_facet | Bekker, Nicolai Sundgaard Toor, Saqib Sohail Sharma, Kamaldeep Pedersen, Thomas Helmer Pedersen, Lars Haastrup |
author_sort | Bekker, Nicolai Sundgaard |
collection | PubMed |
description | In the present study, clover-grass press cake was treated by liquid hot water at temperatures of 180–200 °C for a reaction time of 5–10 min. Evaluation of pretreatments was based on the monosaccharide yield after enzymatic hydrolysis of the pretreated slurry and solid fraction, respectively. Extraction of up to 48% hemicellulose and 4% cellulose was observed during pretreatment. The optimal pretreatment conditions were identified as 190 °C and 10 min resulting in monosaccharide yields of 90% and 73% of the theoretical maximum by slurry and solid conversion, respectively. At optimal conditions, the C6 monosaccharide yield (83–90%) was fairly equal compared to the C5 monosaccharide yield (56–89%), which increased by slurry conversion due to near-complete monomerization of soluble xylo-oligosaccharides. In this study, we showed that clover-grass press cake possesses considerable potential as feedstock for production of fermentable sugars in a biorefinery context. |
format | Online Article Text |
id | pubmed-10391941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103919412023-08-02 Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake Bekker, Nicolai Sundgaard Toor, Saqib Sohail Sharma, Kamaldeep Pedersen, Thomas Helmer Pedersen, Lars Haastrup Heliyon Research Article In the present study, clover-grass press cake was treated by liquid hot water at temperatures of 180–200 °C for a reaction time of 5–10 min. Evaluation of pretreatments was based on the monosaccharide yield after enzymatic hydrolysis of the pretreated slurry and solid fraction, respectively. Extraction of up to 48% hemicellulose and 4% cellulose was observed during pretreatment. The optimal pretreatment conditions were identified as 190 °C and 10 min resulting in monosaccharide yields of 90% and 73% of the theoretical maximum by slurry and solid conversion, respectively. At optimal conditions, the C6 monosaccharide yield (83–90%) was fairly equal compared to the C5 monosaccharide yield (56–89%), which increased by slurry conversion due to near-complete monomerization of soluble xylo-oligosaccharides. In this study, we showed that clover-grass press cake possesses considerable potential as feedstock for production of fermentable sugars in a biorefinery context. Elsevier 2023-07-19 /pmc/articles/PMC10391941/ /pubmed/37534002 http://dx.doi.org/10.1016/j.heliyon.2023.e18448 Text en © 2023 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Bekker, Nicolai Sundgaard Toor, Saqib Sohail Sharma, Kamaldeep Pedersen, Thomas Helmer Pedersen, Lars Haastrup Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake |
title | Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake |
title_full | Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake |
title_fullStr | Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake |
title_full_unstemmed | Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake |
title_short | Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake |
title_sort | optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10391941/ https://www.ncbi.nlm.nih.gov/pubmed/37534002 http://dx.doi.org/10.1016/j.heliyon.2023.e18448 |
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