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High throughput isolation of RNA from single-cells within an intact tissue for spatial and temporal sequencing a reality
Single-cell transcriptomics is essential for understanding biological variability among cells in a heterogenous population. Acquiring high-quality single-cell sequencing data from a tissue sample has multiple challenges including isolation of individual cells as well as amplification of the genetic...
Autores principales: | Stanley, John, Lohith, Akshar, Debiaso, Lucca, Wang, Kevan, Ton, Minh, Cui, Wenwu, Gu, Weiwei, Fu, Aihua, Pourmand, Nader |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393160/ https://www.ncbi.nlm.nih.gov/pubmed/37527243 http://dx.doi.org/10.1371/journal.pone.0289279 |
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