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Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)

The Mormon cricket, Anabrus simplex, is a flightless katydid, one of the major devastating rangeland pests in several states of the western United States. During the past few years, their sudden and periodic outbreaks into massive migratory bands caused significant economic losses to the rangeland f...

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Autores principales: Rana, Seema, Kang, Changsun, Allred, Julianne, Medina-Duran, Jorge Humberto, Canova, Alyssa, Sherry, Danielle, Woller, Derek A, Kim, Dongin, Song, Hojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393271/
https://www.ncbi.nlm.nih.gov/pubmed/37527468
http://dx.doi.org/10.1093/jisesa/iead063
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author Rana, Seema
Kang, Changsun
Allred, Julianne
Medina-Duran, Jorge Humberto
Canova, Alyssa
Sherry, Danielle
Woller, Derek A
Kim, Dongin
Song, Hojun
author_facet Rana, Seema
Kang, Changsun
Allred, Julianne
Medina-Duran, Jorge Humberto
Canova, Alyssa
Sherry, Danielle
Woller, Derek A
Kim, Dongin
Song, Hojun
author_sort Rana, Seema
collection PubMed
description The Mormon cricket, Anabrus simplex, is a flightless katydid, one of the major devastating rangeland pests in several states of the western United States. During the past few years, their sudden and periodic outbreaks into massive migratory bands caused significant economic losses to the rangeland forage and agricultural crops, particularly grain crops. Current population management methods rely heavily on broad-spectrum chemical insecticides, which could be toxic to nontargets, and even the targeted species might develop resistance in the long run. Therefore, we assessed the potential of RNA interference (RNAi)-based alternative management strategies that could supplement the current methods. In insects, RNAi efficiency varies with the method of double-stranded RNA (dsRNA) delivery. We tested 2 different methods of dsRNA delivery: injection and oral feeding of dsRNA. The results showed that Mormon crickets are sensitive to injection of dsRNA in a dose-dependent manner, but refractory to the oral feeding of dsRNA. Further, we confirmed the high nuclease activity in the insect midgut. In order to protect the dsRNA from the dsRNase activity and facilitate its uptake in the midgut, we encapsulated dsRNA inside poly lactic-co-glycolic acid (PLGA) nanoparticles and studied its release kinetics and RNAi efficiency by oral feeding. The release kinetics clearly suggested that the PLGA nanoparticle permeates from the insect digestive system to the hemolymph; however, it failed to induce an efficient RNAi response of the targeted genes. In conclusion, our findings suggest the different responses to dsRNA delivery methods in Mormon crickets, and further investigations involving dsRNA stability and its uptake mechanism are required to use RNAi as an alternative Mormon cricket population management strategy.
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spelling pubmed-103932712023-08-02 Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae) Rana, Seema Kang, Changsun Allred, Julianne Medina-Duran, Jorge Humberto Canova, Alyssa Sherry, Danielle Woller, Derek A Kim, Dongin Song, Hojun J Insect Sci Research The Mormon cricket, Anabrus simplex, is a flightless katydid, one of the major devastating rangeland pests in several states of the western United States. During the past few years, their sudden and periodic outbreaks into massive migratory bands caused significant economic losses to the rangeland forage and agricultural crops, particularly grain crops. Current population management methods rely heavily on broad-spectrum chemical insecticides, which could be toxic to nontargets, and even the targeted species might develop resistance in the long run. Therefore, we assessed the potential of RNA interference (RNAi)-based alternative management strategies that could supplement the current methods. In insects, RNAi efficiency varies with the method of double-stranded RNA (dsRNA) delivery. We tested 2 different methods of dsRNA delivery: injection and oral feeding of dsRNA. The results showed that Mormon crickets are sensitive to injection of dsRNA in a dose-dependent manner, but refractory to the oral feeding of dsRNA. Further, we confirmed the high nuclease activity in the insect midgut. In order to protect the dsRNA from the dsRNase activity and facilitate its uptake in the midgut, we encapsulated dsRNA inside poly lactic-co-glycolic acid (PLGA) nanoparticles and studied its release kinetics and RNAi efficiency by oral feeding. The release kinetics clearly suggested that the PLGA nanoparticle permeates from the insect digestive system to the hemolymph; however, it failed to induce an efficient RNAi response of the targeted genes. In conclusion, our findings suggest the different responses to dsRNA delivery methods in Mormon crickets, and further investigations involving dsRNA stability and its uptake mechanism are required to use RNAi as an alternative Mormon cricket population management strategy. Oxford University Press 2023-08-01 /pmc/articles/PMC10393271/ /pubmed/37527468 http://dx.doi.org/10.1093/jisesa/iead063 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Entomological Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Rana, Seema
Kang, Changsun
Allred, Julianne
Medina-Duran, Jorge Humberto
Canova, Alyssa
Sherry, Danielle
Woller, Derek A
Kim, Dongin
Song, Hojun
Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)
title Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)
title_full Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)
title_fullStr Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)
title_full_unstemmed Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)
title_short Differential responses to double-stranded RNA injection and feeding in Mormon cricket (Orthoptera: Tettigoniidae)
title_sort differential responses to double-stranded rna injection and feeding in mormon cricket (orthoptera: tettigoniidae)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393271/
https://www.ncbi.nlm.nih.gov/pubmed/37527468
http://dx.doi.org/10.1093/jisesa/iead063
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