Cargando…
The potential therapeutic impact of a topical bacteriophage preparation in treating Pseudomonas aeruginosa-infected burn wounds in mice
AIM: This study compared a topical formulation containing lytic phages with a routine antibiotic in the murine model of burn/Pseudomonas aeruginosa infected wound healing. METHODS & MATERIALS: Isolated and purified lytic bacteriophages from hospital sewage were added to the polyethylene glycol (...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393627/ https://www.ncbi.nlm.nih.gov/pubmed/37539104 http://dx.doi.org/10.1016/j.heliyon.2023.e18246 |
_version_ | 1785083202256240640 |
---|---|
author | Piranaghl, Hanieh Golmohammadzadeh, Shiva Soheili, Vahid Noghabi, Zahra Sabeti Memar, Bahram Jalali, Seyede Melika Taherzadeh, Zhila Fazly Bazzaz, Bibi Sedigheh |
author_facet | Piranaghl, Hanieh Golmohammadzadeh, Shiva Soheili, Vahid Noghabi, Zahra Sabeti Memar, Bahram Jalali, Seyede Melika Taherzadeh, Zhila Fazly Bazzaz, Bibi Sedigheh |
author_sort | Piranaghl, Hanieh |
collection | PubMed |
description | AIM: This study compared a topical formulation containing lytic phages with a routine antibiotic in the murine model of burn/Pseudomonas aeruginosa infected wound healing. METHODS & MATERIALS: Isolated and purified lytic bacteriophages from hospital sewage were added to the polyethylene glycol (PEG) based ointment. A second-degree burned wound on the back of twenty-four adult female mice was created. The wounds were infected subcutaneously with 100 μL of 1 × 10(2−3) CFU/mL P. aeruginosa. After 24 h, mice were randomly assigned to one of four groups: mice received a standard antibiotic (antibiotic-treated group), mice received an ointment without bacteriophage (PEG-based group), mice received a PEG-ointment with bacteriophage (bacteriophage-treated group), or mice received no treatment (untreated-control group). Every two days, the contraction of burned wounds, physical activity, and rectal body temperature were recorded. On day 10, mice were sacrificed, and the wounds were cut off and evaluated histopathologically. RESULTS: In ointments containing PEG, bacteriophages were active and stable. The mice receiving bacteriophage and PEG-based ointment had substantially different wound contraction in primary wound healing (P = 0.001). When compared to the control group, the bacteriophage-treated group showed significant variations in wound contraction (P = 0.001). The wound contraction changed significantly between the antibiotic and PEG-based groups (P = 0.002). In all groups, physical activity in mice improved over time, with significant differences (P = 0.001). When the 8th day was compared to the days 2, 4, and 6, significant changes were found (P = 0.001, P = 0.02, and P = 0.02, respectively). Both the positive control and bacteriophage-treated groups showed perfect wound healing histopathologically. However, no significant variations in microscopic histopathological criteria were found between the groups. CONCLUSION: Formulated phage ointment could be a promising approach for treating infected burn wounds infected by P. aeruginosa in mice with no allergic reactions. |
format | Online Article Text |
id | pubmed-10393627 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-103936272023-08-03 The potential therapeutic impact of a topical bacteriophage preparation in treating Pseudomonas aeruginosa-infected burn wounds in mice Piranaghl, Hanieh Golmohammadzadeh, Shiva Soheili, Vahid Noghabi, Zahra Sabeti Memar, Bahram Jalali, Seyede Melika Taherzadeh, Zhila Fazly Bazzaz, Bibi Sedigheh Heliyon Research Article AIM: This study compared a topical formulation containing lytic phages with a routine antibiotic in the murine model of burn/Pseudomonas aeruginosa infected wound healing. METHODS & MATERIALS: Isolated and purified lytic bacteriophages from hospital sewage were added to the polyethylene glycol (PEG) based ointment. A second-degree burned wound on the back of twenty-four adult female mice was created. The wounds were infected subcutaneously with 100 μL of 1 × 10(2−3) CFU/mL P. aeruginosa. After 24 h, mice were randomly assigned to one of four groups: mice received a standard antibiotic (antibiotic-treated group), mice received an ointment without bacteriophage (PEG-based group), mice received a PEG-ointment with bacteriophage (bacteriophage-treated group), or mice received no treatment (untreated-control group). Every two days, the contraction of burned wounds, physical activity, and rectal body temperature were recorded. On day 10, mice were sacrificed, and the wounds were cut off and evaluated histopathologically. RESULTS: In ointments containing PEG, bacteriophages were active and stable. The mice receiving bacteriophage and PEG-based ointment had substantially different wound contraction in primary wound healing (P = 0.001). When compared to the control group, the bacteriophage-treated group showed significant variations in wound contraction (P = 0.001). The wound contraction changed significantly between the antibiotic and PEG-based groups (P = 0.002). In all groups, physical activity in mice improved over time, with significant differences (P = 0.001). When the 8th day was compared to the days 2, 4, and 6, significant changes were found (P = 0.001, P = 0.02, and P = 0.02, respectively). Both the positive control and bacteriophage-treated groups showed perfect wound healing histopathologically. However, no significant variations in microscopic histopathological criteria were found between the groups. CONCLUSION: Formulated phage ointment could be a promising approach for treating infected burn wounds infected by P. aeruginosa in mice with no allergic reactions. Elsevier 2023-07-13 /pmc/articles/PMC10393627/ /pubmed/37539104 http://dx.doi.org/10.1016/j.heliyon.2023.e18246 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Piranaghl, Hanieh Golmohammadzadeh, Shiva Soheili, Vahid Noghabi, Zahra Sabeti Memar, Bahram Jalali, Seyede Melika Taherzadeh, Zhila Fazly Bazzaz, Bibi Sedigheh The potential therapeutic impact of a topical bacteriophage preparation in treating Pseudomonas aeruginosa-infected burn wounds in mice |
title | The potential therapeutic impact of a topical bacteriophage preparation in treating Pseudomonas aeruginosa-infected burn wounds in mice |
title_full | The potential therapeutic impact of a topical bacteriophage preparation in treating Pseudomonas aeruginosa-infected burn wounds in mice |
title_fullStr | The potential therapeutic impact of a topical bacteriophage preparation in treating Pseudomonas aeruginosa-infected burn wounds in mice |
title_full_unstemmed | The potential therapeutic impact of a topical bacteriophage preparation in treating Pseudomonas aeruginosa-infected burn wounds in mice |
title_short | The potential therapeutic impact of a topical bacteriophage preparation in treating Pseudomonas aeruginosa-infected burn wounds in mice |
title_sort | potential therapeutic impact of a topical bacteriophage preparation in treating pseudomonas aeruginosa-infected burn wounds in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393627/ https://www.ncbi.nlm.nih.gov/pubmed/37539104 http://dx.doi.org/10.1016/j.heliyon.2023.e18246 |
work_keys_str_mv | AT piranaghlhanieh thepotentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT golmohammadzadehshiva thepotentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT soheilivahid thepotentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT noghabizahrasabeti thepotentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT memarbahram thepotentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT jalaliseyedemelika thepotentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT taherzadehzhila thepotentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT fazlybazzazbibisedigheh thepotentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT piranaghlhanieh potentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT golmohammadzadehshiva potentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT soheilivahid potentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT noghabizahrasabeti potentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT memarbahram potentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT jalaliseyedemelika potentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT taherzadehzhila potentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice AT fazlybazzazbibisedigheh potentialtherapeuticimpactofatopicalbacteriophagepreparationintreatingpseudomonasaeruginosainfectedburnwoundsinmice |