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Colloidal silver against macrophage infections and biofilms of atypical mycobacteria
Skin and soft tissue infection (SSTI) caused by atypical mycobacteria such as Mycobacterium abscessus and Mycobacterium avium intracellulare complex (MAIC) have increased in recent years. Current therapeutic options are limited, and hence new and better therapies are urgently required. Colloidal Sil...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393856/ https://www.ncbi.nlm.nih.gov/pubmed/36729280 http://dx.doi.org/10.1007/s10534-023-00494-w |
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author | Feizi, Sholeh Cooksley, Clare M. Ramezanpour, Mahnaz Nepal, Roshan Psaltis, Alkis J. Wormald, Peter-John Vreugde, Sarah |
author_facet | Feizi, Sholeh Cooksley, Clare M. Ramezanpour, Mahnaz Nepal, Roshan Psaltis, Alkis J. Wormald, Peter-John Vreugde, Sarah |
author_sort | Feizi, Sholeh |
collection | PubMed |
description | Skin and soft tissue infection (SSTI) caused by atypical mycobacteria such as Mycobacterium abscessus and Mycobacterium avium intracellulare complex (MAIC) have increased in recent years. Current therapeutic options are limited, and hence new and better therapies are urgently required. Colloidal Silver (CS) has been identified for its widespread antibacterial properties and silver-impregnated dressings have been used for SSTIs caused by various pathogens. The efficacy of Green Synthesized Colloidal Silver (GSCS) was investigated for bacterial growth inhibition (BGI) using a microdilution method and minimum biofilm eradication concentration (MBEC) using resazurin assay and confocal scanning laser microscopy (CSLM) of M. abscessus (n = 5) and MAIC (n = 5). The antibacterial effect of GSCS against M. abscessus infected macrophages was also evaluated. The in vitro cytotoxicity of GSCS on a human keratinocyte cell line (HaCaT) and neonatal foreskin fibroblasts was analyzed by the crystal violet proliferation assay. Average BGI and MBEC of GSCS varied between 0.7 and 22 ppm for M. abscessus and MAIC. The concentration of 3 ppm reduced M. abscessus-infection in macrophages significantly. GSCS was not cytotoxic to HaCaT and neonatal foreskin fibroblast cells at concentrations < 3 ppm up to 2 h exposure time. GSCS therefore, has the potential for topical application against atypical mycobacterial SSTI. |
format | Online Article Text |
id | pubmed-10393856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-103938562023-08-03 Colloidal silver against macrophage infections and biofilms of atypical mycobacteria Feizi, Sholeh Cooksley, Clare M. Ramezanpour, Mahnaz Nepal, Roshan Psaltis, Alkis J. Wormald, Peter-John Vreugde, Sarah Biometals Article Skin and soft tissue infection (SSTI) caused by atypical mycobacteria such as Mycobacterium abscessus and Mycobacterium avium intracellulare complex (MAIC) have increased in recent years. Current therapeutic options are limited, and hence new and better therapies are urgently required. Colloidal Silver (CS) has been identified for its widespread antibacterial properties and silver-impregnated dressings have been used for SSTIs caused by various pathogens. The efficacy of Green Synthesized Colloidal Silver (GSCS) was investigated for bacterial growth inhibition (BGI) using a microdilution method and minimum biofilm eradication concentration (MBEC) using resazurin assay and confocal scanning laser microscopy (CSLM) of M. abscessus (n = 5) and MAIC (n = 5). The antibacterial effect of GSCS against M. abscessus infected macrophages was also evaluated. The in vitro cytotoxicity of GSCS on a human keratinocyte cell line (HaCaT) and neonatal foreskin fibroblasts was analyzed by the crystal violet proliferation assay. Average BGI and MBEC of GSCS varied between 0.7 and 22 ppm for M. abscessus and MAIC. The concentration of 3 ppm reduced M. abscessus-infection in macrophages significantly. GSCS was not cytotoxic to HaCaT and neonatal foreskin fibroblast cells at concentrations < 3 ppm up to 2 h exposure time. GSCS therefore, has the potential for topical application against atypical mycobacterial SSTI. Springer Netherlands 2023-02-02 2023 /pmc/articles/PMC10393856/ /pubmed/36729280 http://dx.doi.org/10.1007/s10534-023-00494-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Feizi, Sholeh Cooksley, Clare M. Ramezanpour, Mahnaz Nepal, Roshan Psaltis, Alkis J. Wormald, Peter-John Vreugde, Sarah Colloidal silver against macrophage infections and biofilms of atypical mycobacteria |
title | Colloidal silver against macrophage infections and biofilms of atypical mycobacteria |
title_full | Colloidal silver against macrophage infections and biofilms of atypical mycobacteria |
title_fullStr | Colloidal silver against macrophage infections and biofilms of atypical mycobacteria |
title_full_unstemmed | Colloidal silver against macrophage infections and biofilms of atypical mycobacteria |
title_short | Colloidal silver against macrophage infections and biofilms of atypical mycobacteria |
title_sort | colloidal silver against macrophage infections and biofilms of atypical mycobacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393856/ https://www.ncbi.nlm.nih.gov/pubmed/36729280 http://dx.doi.org/10.1007/s10534-023-00494-w |
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