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TRIM28 modulates nuclear receptor signaling to regulate uterine function
Estrogen and progesterone, acting through their cognate receptors the estrogen receptor α (ERα) and the progesterone receptor (PR) respectively, regulate uterine biology. Using rapid immunoprecipitation and mass spectrometry (RIME) and co-immunoprecipitation, we identified TRIM28 (Tripartite motif c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393996/ https://www.ncbi.nlm.nih.gov/pubmed/37528140 http://dx.doi.org/10.1038/s41467-023-40395-7 |
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author | Li, Rong Wang, Tianyuan Marquardt, Ryan M. Lydon, John P. Wu, San-Pin DeMayo, Francesco J. |
author_facet | Li, Rong Wang, Tianyuan Marquardt, Ryan M. Lydon, John P. Wu, San-Pin DeMayo, Francesco J. |
author_sort | Li, Rong |
collection | PubMed |
description | Estrogen and progesterone, acting through their cognate receptors the estrogen receptor α (ERα) and the progesterone receptor (PR) respectively, regulate uterine biology. Using rapid immunoprecipitation and mass spectrometry (RIME) and co-immunoprecipitation, we identified TRIM28 (Tripartite motif containing 28) as a protein which complexes with ERα and PR in the regulation of uterine function. Impairment of TRIM28 expression results in the inability of the uterus to support early pregnancy through altered PR and ERα action in the uterine epithelium and stroma by suppressing PR and ERα chromatin binding. Furthermore, TRIM28 ablation in PR-expressing uterine cells results in the enrichment of a subset of TRIM28 positive and PR negative pericytes and epithelial cells with progenitor potential. In summary, our study reveals the important roles of TRIM28 in regulating endometrial cell composition and function in women, and also implies its critical functions in other hormone regulated systems. |
format | Online Article Text |
id | pubmed-10393996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-103939962023-08-03 TRIM28 modulates nuclear receptor signaling to regulate uterine function Li, Rong Wang, Tianyuan Marquardt, Ryan M. Lydon, John P. Wu, San-Pin DeMayo, Francesco J. Nat Commun Article Estrogen and progesterone, acting through their cognate receptors the estrogen receptor α (ERα) and the progesterone receptor (PR) respectively, regulate uterine biology. Using rapid immunoprecipitation and mass spectrometry (RIME) and co-immunoprecipitation, we identified TRIM28 (Tripartite motif containing 28) as a protein which complexes with ERα and PR in the regulation of uterine function. Impairment of TRIM28 expression results in the inability of the uterus to support early pregnancy through altered PR and ERα action in the uterine epithelium and stroma by suppressing PR and ERα chromatin binding. Furthermore, TRIM28 ablation in PR-expressing uterine cells results in the enrichment of a subset of TRIM28 positive and PR negative pericytes and epithelial cells with progenitor potential. In summary, our study reveals the important roles of TRIM28 in regulating endometrial cell composition and function in women, and also implies its critical functions in other hormone regulated systems. Nature Publishing Group UK 2023-08-01 /pmc/articles/PMC10393996/ /pubmed/37528140 http://dx.doi.org/10.1038/s41467-023-40395-7 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Li, Rong Wang, Tianyuan Marquardt, Ryan M. Lydon, John P. Wu, San-Pin DeMayo, Francesco J. TRIM28 modulates nuclear receptor signaling to regulate uterine function |
title | TRIM28 modulates nuclear receptor signaling to regulate uterine function |
title_full | TRIM28 modulates nuclear receptor signaling to regulate uterine function |
title_fullStr | TRIM28 modulates nuclear receptor signaling to regulate uterine function |
title_full_unstemmed | TRIM28 modulates nuclear receptor signaling to regulate uterine function |
title_short | TRIM28 modulates nuclear receptor signaling to regulate uterine function |
title_sort | trim28 modulates nuclear receptor signaling to regulate uterine function |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10393996/ https://www.ncbi.nlm.nih.gov/pubmed/37528140 http://dx.doi.org/10.1038/s41467-023-40395-7 |
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