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Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trial

Most multigene mutation tests require tissue specimens. However, cytological specimens are easily obtained in the clinical practice and provide high‐quality DNA and RNA. We aimed to establish a test that utilizes cytological specimens and performed a multi‐institutional study to investigate the perf...

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Autores principales: Fujita, Kazutaka, Arai, Ryo, Shoji, Satoshi, Saito, Ryota, Nomura, Motoko, Hotta, Takamasa, Asahina, Hajime, Kawakami, Masanori, Nakachi, Ichiro, Hasegawa, Yukihiro, Okafuji, Kohei, Suzuki, Aya, Miyanaga, Akihiko, Sunaga, Noriaki, Nagashima, Hiromi, Ikeda, Naoya, Watanabe, Satoshi, Nagai, Yoshiaki, Furuta, Megumi, Kage, Hidenori, Arai, Daisuke, Fukuhara, Tatsuro, Nakayama, Masayuki, Morita, Satoshi, Kobayashi, Kunihiko, Hagiwara, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10394136/
https://www.ncbi.nlm.nih.gov/pubmed/37139543
http://dx.doi.org/10.1111/cas.15831
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author Fujita, Kazutaka
Arai, Ryo
Shoji, Satoshi
Saito, Ryota
Nomura, Motoko
Hotta, Takamasa
Asahina, Hajime
Kawakami, Masanori
Nakachi, Ichiro
Hasegawa, Yukihiro
Okafuji, Kohei
Suzuki, Aya
Miyanaga, Akihiko
Sunaga, Noriaki
Nagashima, Hiromi
Ikeda, Naoya
Watanabe, Satoshi
Nagai, Yoshiaki
Furuta, Megumi
Kage, Hidenori
Arai, Daisuke
Fukuhara, Tatsuro
Nakayama, Masayuki
Morita, Satoshi
Kobayashi, Kunihiko
Hagiwara, Koichi
author_facet Fujita, Kazutaka
Arai, Ryo
Shoji, Satoshi
Saito, Ryota
Nomura, Motoko
Hotta, Takamasa
Asahina, Hajime
Kawakami, Masanori
Nakachi, Ichiro
Hasegawa, Yukihiro
Okafuji, Kohei
Suzuki, Aya
Miyanaga, Akihiko
Sunaga, Noriaki
Nagashima, Hiromi
Ikeda, Naoya
Watanabe, Satoshi
Nagai, Yoshiaki
Furuta, Megumi
Kage, Hidenori
Arai, Daisuke
Fukuhara, Tatsuro
Nakayama, Masayuki
Morita, Satoshi
Kobayashi, Kunihiko
Hagiwara, Koichi
author_sort Fujita, Kazutaka
collection PubMed
description Most multigene mutation tests require tissue specimens. However, cytological specimens are easily obtained in the clinical practice and provide high‐quality DNA and RNA. We aimed to establish a test that utilizes cytological specimens and performed a multi‐institutional study to investigate the performance of MINtS, a test based on next‐generation sequencing. A standard procedure for specimen isolation was defined. The specimens were considered suitable for the test if >100 ng DNA and >50 ng RNA could be extracted from them. In total, 500 specimens from 19 institutions were investigated. MINtS detected druggable mutations in 63% (136 of 222) of adenocarcinomas. Discordant results between MINtS and the companion diagnostics were observed in 14 of 310 specimens for the EGFR gene, and 6 of 339 specimens for the ALK fusion genes. Confirmation by other companion diagnostics for the EGFR mutations or the clinical response to an ALK inhibitor all supported the results obtained by MINtS. MINtS along with the isolation procedure presented in the current study will be a platform to establish multigene mutation tests that utilize cytological specimens. UMIN000040415.
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spelling pubmed-103941362023-08-03 Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trial Fujita, Kazutaka Arai, Ryo Shoji, Satoshi Saito, Ryota Nomura, Motoko Hotta, Takamasa Asahina, Hajime Kawakami, Masanori Nakachi, Ichiro Hasegawa, Yukihiro Okafuji, Kohei Suzuki, Aya Miyanaga, Akihiko Sunaga, Noriaki Nagashima, Hiromi Ikeda, Naoya Watanabe, Satoshi Nagai, Yoshiaki Furuta, Megumi Kage, Hidenori Arai, Daisuke Fukuhara, Tatsuro Nakayama, Masayuki Morita, Satoshi Kobayashi, Kunihiko Hagiwara, Koichi Cancer Sci ORIGINAL ARTICLES Most multigene mutation tests require tissue specimens. However, cytological specimens are easily obtained in the clinical practice and provide high‐quality DNA and RNA. We aimed to establish a test that utilizes cytological specimens and performed a multi‐institutional study to investigate the performance of MINtS, a test based on next‐generation sequencing. A standard procedure for specimen isolation was defined. The specimens were considered suitable for the test if >100 ng DNA and >50 ng RNA could be extracted from them. In total, 500 specimens from 19 institutions were investigated. MINtS detected druggable mutations in 63% (136 of 222) of adenocarcinomas. Discordant results between MINtS and the companion diagnostics were observed in 14 of 310 specimens for the EGFR gene, and 6 of 339 specimens for the ALK fusion genes. Confirmation by other companion diagnostics for the EGFR mutations or the clinical response to an ALK inhibitor all supported the results obtained by MINtS. MINtS along with the isolation procedure presented in the current study will be a platform to establish multigene mutation tests that utilize cytological specimens. UMIN000040415. John Wiley and Sons Inc. 2023-05-03 /pmc/articles/PMC10394136/ /pubmed/37139543 http://dx.doi.org/10.1111/cas.15831 Text en © 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle ORIGINAL ARTICLES
Fujita, Kazutaka
Arai, Ryo
Shoji, Satoshi
Saito, Ryota
Nomura, Motoko
Hotta, Takamasa
Asahina, Hajime
Kawakami, Masanori
Nakachi, Ichiro
Hasegawa, Yukihiro
Okafuji, Kohei
Suzuki, Aya
Miyanaga, Akihiko
Sunaga, Noriaki
Nagashima, Hiromi
Ikeda, Naoya
Watanabe, Satoshi
Nagai, Yoshiaki
Furuta, Megumi
Kage, Hidenori
Arai, Daisuke
Fukuhara, Tatsuro
Nakayama, Masayuki
Morita, Satoshi
Kobayashi, Kunihiko
Hagiwara, Koichi
Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trial
title Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trial
title_full Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trial
title_fullStr Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trial
title_full_unstemmed Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trial
title_short Detection of multiple druggable mutations of lung cancer from cytology specimens by MINtS: An advanced medicine A trial
title_sort detection of multiple druggable mutations of lung cancer from cytology specimens by mints: an advanced medicine a trial
topic ORIGINAL ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10394136/
https://www.ncbi.nlm.nih.gov/pubmed/37139543
http://dx.doi.org/10.1111/cas.15831
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