Sinomonas terrae sp. nov., Isolated from an Agricultural Soil

While searching for the bacteria which are responsible for degradation of pesticide in soybean field soil, a novel bacterial strain, designated 5-5(T), was isolated. The cells of the strain were Gram-staining-positive, aerobic and non-motile rods. Growth occurred at 10-42°C (optimum, 30°C), pH 5.5-9.0 (optimum, pH 7.0-7.5), and 0-2% (w/v) NaCl (optimum, 1%). The predominant fatty acids were C(15:0) anteiso, C(17:0) anteiso, and summed feature 8 (C(18:1) ω7c and/or C(18:1) ω6c). The predominant menaquinone was MK-9 (H(2)). Diphosphatidylglycerol, glycolipids, phosphatidylinositol, and phosphatidylglycerol were the major polar lipids. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain 5-5(T) is a member of the genus Sinomonas and its closest relative is Sinomonas humi MUSC 117(T), sharing a genetic similarity of 98.4%. The draft genome of strain 5-5(T) was 4,727,205 bp long with an N50 contig of 4,464,284 bp. Genomic DNA G+C content of strain 5-5(T) was 68.0 mol%. The average nucleotide identity (ANI) values between strain 5-5(T) and its closest strains S. humi MUSC 117(T) and S. susongensis A31(T) were 87.0, and 84.3 % respectively. In silico DNA-DNA hybridization values between strain 5-5(T) and its closest strains S. humi MUSC 117(T) and S. susongensis A31(T) were 32.5% and 27.9% respectively. Based on the ANI and in silico DNA-DNA hybridization analyses, the 5-5(T) strain was considered as novel species belonging to the genus Sinomonas. On the basis of the results from phenotypic, genotypic and chemotaxonomic analyses, strain 5-5(T) represents a novel species of the genus Sinomonas, for which the name Sinomonas terrae sp. nov. is proposed. The type strain is 5-5(T) (=KCTC 49650(T)=NBRC 115790(T)).