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Genomic mining and diversity of assembly line polyketide synthases

Assembly line polyketide synthases (PKSs) are a large family of multifunctional enzymes responsible for synthesizing many medicinally relevant natural products with remarkable structural variety and biological activity. The decrease in cost of genomic sequencing paired with development of computatio...

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Detalles Bibliográficos
Autores principales: Kishore, Shreya, Khosla, Chaitan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10394414/
https://www.ncbi.nlm.nih.gov/pubmed/37528731
http://dx.doi.org/10.1098/rsob.230096
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author Kishore, Shreya
Khosla, Chaitan
author_facet Kishore, Shreya
Khosla, Chaitan
author_sort Kishore, Shreya
collection PubMed
description Assembly line polyketide synthases (PKSs) are a large family of multifunctional enzymes responsible for synthesizing many medicinally relevant natural products with remarkable structural variety and biological activity. The decrease in cost of genomic sequencing paired with development of computational tools like antiSMASH presents an opportunity to survey the vast diversity of assembly line PKS. Mining the genomic data in the National Center for Biotechnology Information database, our updated catalogue (https://orphanpkscatalog2022.stanford.edu/catalog) presented in this article revealed 8799 non-redundant assembly line polyketide synthase clusters across 4083 species, representing a threefold increase over the past 4 years. Additionally, 95% of the clusters are ‘orphan clusters' for which natural products are neither chemically nor biologically characterized. Our analysis indicates that the diversity of assembly line PKSs remains vastly under-explored and also highlights the promise of a genomics-driven approach to natural product discovery.
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spelling pubmed-103944142023-08-03 Genomic mining and diversity of assembly line polyketide synthases Kishore, Shreya Khosla, Chaitan Open Biol Commentary Assembly line polyketide synthases (PKSs) are a large family of multifunctional enzymes responsible for synthesizing many medicinally relevant natural products with remarkable structural variety and biological activity. The decrease in cost of genomic sequencing paired with development of computational tools like antiSMASH presents an opportunity to survey the vast diversity of assembly line PKS. Mining the genomic data in the National Center for Biotechnology Information database, our updated catalogue (https://orphanpkscatalog2022.stanford.edu/catalog) presented in this article revealed 8799 non-redundant assembly line polyketide synthase clusters across 4083 species, representing a threefold increase over the past 4 years. Additionally, 95% of the clusters are ‘orphan clusters' for which natural products are neither chemically nor biologically characterized. Our analysis indicates that the diversity of assembly line PKSs remains vastly under-explored and also highlights the promise of a genomics-driven approach to natural product discovery. The Royal Society 2023-08-02 /pmc/articles/PMC10394414/ /pubmed/37528731 http://dx.doi.org/10.1098/rsob.230096 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited.
spellingShingle Commentary
Kishore, Shreya
Khosla, Chaitan
Genomic mining and diversity of assembly line polyketide synthases
title Genomic mining and diversity of assembly line polyketide synthases
title_full Genomic mining and diversity of assembly line polyketide synthases
title_fullStr Genomic mining and diversity of assembly line polyketide synthases
title_full_unstemmed Genomic mining and diversity of assembly line polyketide synthases
title_short Genomic mining and diversity of assembly line polyketide synthases
title_sort genomic mining and diversity of assembly line polyketide synthases
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10394414/
https://www.ncbi.nlm.nih.gov/pubmed/37528731
http://dx.doi.org/10.1098/rsob.230096
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